LOCUS       sequence-466452-        7359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the voltage sensor QuasAr2 in cholinergic neurons of C. 
            elegans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Bergs ACF, Liewald JF, Rodriguez-Rozada S, Liu Q, Wirt C, Bessel A, 
            Zeitzschel N, Durmaz H, Nozownik A, Dill H, Jospin M, Vierock J, 
            Bargmann CI, Hegemann P, Wiegert JS, Gottschalk A
  TITLE     All-optical closed-loop voltage clamp for precise control of muscles
            and neurons in live animals.
  JOURNAL   Nat Commun. 2023 Apr 6;14(1):1939. doi: 10.1038/s41467-023-37622-6.
  PUBMED    37024493
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-6"; volume: "14(1):1939. doi"; pages: " 
            10.1038/s41467-023-37622-6"
COMMENT     Sequence Label: pAB17 (punc-17::QuasAr)
FEATURES             Location/Qualifiers
     source          1..7359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4933..4955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5055..5074
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5152..5170)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5238..5342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5343..5411,5412..6203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5561..5580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6374..6962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6863..6882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7116..7133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7250..7271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7286..7291,7292..7309,7310..7316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7324..7340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7329..7351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7348..7359,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7348..7359,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgct cccggccgcc atggccgcgg gattacacca
       61 atcatttctc ccctccgaaa tgtgtagtcg tcatgacaaa gtggtgacac tggattacgg
      121 taggctccgc ccacttcttg gaaaagtgac gtgggctcgg tgagccctaa agcttttggt
      181 tttcacaatt ttctggtttt tgggattttt gcgggaattt tgagaggaga aagttgggag
      241 agaagatatt ggaatggtcc ccggggagct gtacatttat acatctagac tgaagctggg
      301 gtattgcaaa aagagaaaag cctatcactt tctttccatc ctgcatactt gccaaaaagt
      361 tttggctgag cttattttta aaattggaat tttttggaat tttcagaaaa gaaaaagttt
      421 tttctttttt tttaatttaa aaaatttttg ctatttattg catggtacta cagaaaaact
      481 tgaatgaaat aaaaaatttg gaaaattcaa gaaaactttt tttttcatag tttatttatt
      541 cgagttttaa tgtggctttt attttgttcg gtttttgttt taaaaagatt ttatacaaaa
      601 tttcaagaat ttacaatttt tccgttgtaa aaaatccaaa aaaaaatcca aaaaaatagt
      661 tcagaccttt ttttaaattt taaatttaaa aattcaatac caaaaaatta aaaaaaatta
      721 gtttttccga gaataaagga aaaatgttga aaaaatatta tatataaaat ttccagaaaa
      781 tgtgaaaact ccaaaaaact gcagactttt ccccaaacta gctatttttt gaattcatta
      841 cctttaaagg tggagtaccg aaatttaaga ctttgctttt ttagacccaa aatggtccaa
      901 aactaccaaa tttcgtaatg agacgttctg aaaatttttc aaaaaaatgt tatgggagct
      961 caaagttttt gaaaaaatgg tatattttta gctaatttta accacttctt ggcgtcgcag
     1021 cggttggaac tcaattttaa ttaaattatc atccttcaat gtccttcaat ctttgcaatt
     1081 tcaatcgacc atacgcgttc agagcatttt tttttttcgt ttttcctccc gtccagcaca
     1141 tatttaaata ttctctctcc ccctcccctg gttcctaaaa acttgggaaa ggggaatttc
     1201 gaaggaggtg gaagcttctt cttctccccg agcacacaca cacatcctct cttcaaaatc
     1261 tttaagaaat agtgcgtgtg aatgactaga cgctgaagct aacttggttt ccgcgaacaa
     1321 tcccaatcag tgcctgtgcc gaggcttcgg agaagaagag attgacgtcg tctttttctt
     1381 tttttgtgaa acagaataaa aaagaatacg aaatggactt tttcacacat tttcgttact
     1441 tttccagaaa aaaacgaagc tctagccatg gaagtttgaa ttgaaaacct aaaccccgat
     1501 gtgtattcat ttgagcttaa gcctaagcct aattctacgc ctaagcataa gcctaagcct
     1561 atgttcaagc ctaagccaaa ttgtagaacg ttaccaaaag atcaaaccta tttttcctac
     1621 agaaaaggtc taatgataac tgttaagcga gagacaagga gagatatata tcaaaaacta
     1681 gcccaaactg tgattaacat ctattaactg acctcaaaaa aaaaaatagt agagattaaa
     1741 tactgctcaa aaagtttctt ggaaaagtaa attgttaggt ttctggggcc tgtaaaactg
     1801 gaccgagttt tgaagaattt ttttaacttt tcaaagtttg gcgtcactga aagctttata
     1861 ttgtacatgt tccaccaatt ttcttttgta tccgtttttt ctgtctcaaa acggagctcg
     1921 agcccatgac gtgtggatac cgtgagaatc cgcccaattc cttcacatcg atctcatatt
     1981 tctttcccag tttatattga attcccgttg aattgcggct ttgcagggga gaagatgtgc
     2041 gtctttatat ttttttttct atccttaccc gccgtgaatc cggctcgaat tgagccaatt
     2101 tttatgaata gtagagctaa ttgacttcgt ctccctagaa ttgatgaggt aaagctcgtg
     2161 gggagatatg gagatttttc gatttttttt ccagtaattt tcagttcaaa atttgagctt
     2221 ttgagcagga gttttgagat ttgtgccaaa atttcctatt attttttcaa atatttttgc
     2281 ttttgtaaaa aaggtgtagt agaagttttg tagtgccact attttttttt tgaaaattat
     2341 gttttggcgg aaagttcttt aaaaaagtta atcaaaattt ttggcgcttt agtcaaatta
     2401 attttctggc aaaaatatta gtaattttgg cgtgagttca attttcccat tcttaaaatt
     2461 tcacacaatt aagaatttta agatttgggt ttttttttca acaaaaaatc aaatatttac
     2521 atgaaaattc ggcgggaaat aaaaaaaaat attccgattc aatgtgtgaa taactttttg
     2581 cggggaattc aaatttgcat tatttaaact ttttttaagc tcgaaaaatt gaaattctta
     2641 aataaataaa taaaattttc ctcgaatttt gaatgtttcg attttttagc gttatttttt
     2701 caaaatttcc actaaaactc tagttctaaa gttctaaaac tttaaaaact tcctaatgtt
     2761 gtggtcttgt gtttttttgg cagcgatttc caaatttctg aaattccaac aaaaaaacca
     2821 ctacgtcagt ttgcatatcc aacccccccc cccccccccc cccctctgag ccaatcaatc
     2881 atgtgcattt tgaattggcg ccaaggcaat cttcgtaaaa aataacaata gagtcatccc
     2941 tcagaacaga aaagaaaccc gcccgggggc tcaaatacac atgtcatttt tcttttttcg
     3001 cctgattttt agcctaaatt agtgtttagc aattttttaa ggtaactcgg tggagtcatt
     3061 tgaacttttt aaactaaaat ttcagaaaaa attctttttt tttgtcaaaa aataattttc
     3121 ccgccggaat ttttcaaaaa aataattttt attttatttt tcaagttcct tgtttcctct
     3181 ttattttttt attttgcccc caaaagtttt acactaaaat atttaatttt caggtaattc
     3241 atcgaaagtc tttctatttt ccgcatctct tgttcaaggg attggccaaa ggacccaaag
     3301 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggatggta
     3361 agtatcgctc tgcaggctgg ttacgaccta ctgggtgacg gcagacctga aaccctgtgg
     3421 ctgggcatcg gcactctgct gatgctgatt ggaaccttct actttctggt ccgcggatgg
     3481 ggagtcaccg ataaggatgc ccgggaatat tacgctgtga ctatcctggt gtccggaatc
     3541 gcatccgccg catatctgtc tatgttcttt ggtatcgggc ttactgaggt gtccgtcggg
     3601 ggcgaaatgt tggatatcta ttatgccagg tacgcccagt ggctgtttac caccccactt
     3661 ctgctgctgc acctggccct tctcgctaag gtggatcggg tgaccatcgg caccctggtg
     3721 ggtgtggacg ccctgatgat cgtcactggc ctcatcggag ccttgagcca cacggccata
     3781 gccagataca gttggtggtt gttctctaca atttgcatga tagtggtgct ctatgttctg
     3841 gctacatccc tgcgatctgc tgcaaaggag cggggccccg aggtggcatc tacctttaac
     3901 accctgacag ctctggtctt ggtgctgtgg accgcttacc ctatcctgtg gatcataggc
     3961 actgagggcg ctggcgtggt gggcctgggc atcgaaactc tgctgtttat ggtgttggac
     4021 gtgactgcca aggtcggctt tggctttatc ctgttgagat cccgggctat tctgggcgac
     4081 accgagcgtc gacggtacca tggtattgat atctgagctc cgcatcggcc gctgtcatca
     4141 gatcgccatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     4201 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     4261 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     4321 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     4381 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     4441 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     4501 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     4561 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     4621 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     4681 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     4741 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     4801 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     4861 aaacataact gtttctacta gtcggccgta cgggcccttt cgtctcgcgc gtttcggtga
     4921 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4981 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5041 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5101 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cggccttaag ggcctcgtga
     5161 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5221 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5281 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5341 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5401 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5461 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5521 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5581 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5641 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5701 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5761 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5821 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5881 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5941 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6001 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6061 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6121 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6181 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6241 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6301 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6361 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6421 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6481 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6541 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6601 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6661 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6721 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7141 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7201 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7261 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7321 gaattgtgag cggataacaa tttcacacag gaaacagct
//
