LOCUS       sequence-466491-       15889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  D. rerio elavl3 promoter driving expression of human Arrestin-TEV 
            protease fusion protein for use in trans-Tango..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Coomer CE, Naumova D, Talay M, Zolyomi B, Snell NJ, Sorkac A, 
            Chanchu JM, Cheng J, Roman I, Li J, Robson D, McLean DL, Barnea G, 
            Halpern ME
  TITLE     Transsynaptic labeling and transcriptional control of zebrafish 
            neural circuits.
  JOURNAL   Nat Neurosci. 2025 Jan;28(1):189-200. doi: 
            10.1038/s41593-024-01815-z. Epub 2024 Dec 19.
  PUBMED    39702668
REFERENCE   2  (bases 1 to 15889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-024-01815-z"; journalName: "Nat Neurosci"; date: 
            "2025-01"; volume: "28"; issue: "1"; pages: "189-200"
COMMENT     Sequence Label: elavl3:hArr-TEV;he1.1:YFP
FEATURES             Location/Qualifiers
     source          1..15889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(152..163,164..326)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    842..866
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9572..9596)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10835..11542
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GSSLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTSQEAQQWVSGWRLNADSVLWGGHKVFMSKPE
                     EPFQPVKEATQLMN"
     polyA_signal    11594..11728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11618..11637)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11672..11691
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             join(12357..12359,12360..12362,12363..13076)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12402..12423)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12663..12682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13010..13031
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(13314..13331)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13320..13545
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    join(13874..14061,14062..14073)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(14099..14116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14270..14858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14350..14369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15029..15820,15821..15889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15652..15671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgagg
      121 taccgatcgg tatctgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt
      181 actcaagtga aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct
      241 ggctagaatc ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat
      301 taaggaagta aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc
      361 tcaaaaaaca ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt
      421 taattatcaa actataagac agacaatcta atgccagtac acgctactca aagttgtaaa
      481 acctcagatt taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc
      541 ttttggggct gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg
      601 gaagaggtgt attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact
      661 ccctggtgtc tgaaacacag gccagatggg ccattcgtac gcttaatttc gaatatcgat
      721 atcaagggcc catctcgagt atccggacac ctaggtagca tgcatggcgc cttgtcgact
      781 agctagcatc ccgggaagaa ttcctcgacg gatccaccgg tgtggaattc tgcagatatc
      841 aacaagtttg tacaaaaaag caggctctat tcctaaagac cttgggtgac caaaatctta
      901 ttttaataaa taaaactgtt tattaaaact tttttgtttc aaagaaccat atgtatagtg
      961 aaatttataa aaatatcaat ttttaaaaag ctggtgtact catttatgtt atgaactcta
     1021 aaaccatata ctgactgcaa gtgatgatgt atagagtgat gtttacgagt aaacatattt
     1081 agttgtatac atcctactga gcacattttg atgtatgaaa taacattaca agctttatcc
     1141 aaattaagcc attttaaaac actgccaatt gaaaatacaa atcctggaaa aaatcgtctt
     1201 tagcgcagtc atttgagcca tcctaatccg ttacctcaga ccataataag aagggataac
     1261 actagctgta gcaatggaac acatctgttt cacacaatca tatctcctgc gccggtgcta
     1321 agcagattca gcgtgatcat aacatgcttt ccactcataa atgtaaattt acaatttgca
     1381 catgtaaaac agacactttt gagatattgg ataaaaaaac aagagtatat tgcttagttt
     1441 catccaccag tcatccccac agcgtttgga aggccataaa aagtgtctaa aatcaatgat
     1501 cattgaaaga gcacaagaga gactcttacg ctgtaatgcc actggggaca aaagtgacag
     1561 tctcttaatg ggctcttctg gaggggctcc tgaacattaa aaattatcag cgaaattacc
     1621 gaaagagctt caagcaactg gcatgcttga tcctctgcgt cggggcggtg aataggtgct
     1681 tcagatgccc tcttacccac gggctggatt cagctgcccc gctaccagcg gagaccccct
     1741 aatgagcctc tgcaattaag tttattcatg ttaagtgtga acggggtgcg tgcggaactg
     1801 tgggcagcta acagacctgg gttctttgtg ccacaagtgc tgcctttatt cggctcacaa
     1861 agcagaaaac aacacccgca cctataatgg cgccctcggc tgggtctaag aaacgtggcg
     1921 agttgacaga gcagagtggg cggggttaag acagactgac agcgggaccc atctccatcc
     1981 tcttattaac gcttaacgag tgccttcctc atgcaatatt catcgccact aatatcatcc
     2041 aagctctgag ctgagctggc cacttatgta aggcaattat gtaaaatatc agacagggcc
     2101 cacactcaga atctgactgg ggtagagacg cgggacgaga accgagagca agaactgaaa
     2161 gtgaaagtga ccactaaagg gaggagagga cagaggggca ggatgtgtca agattaccag
     2221 agaacacttg gccagaaatg cgcaaccatt ggagctctcc ggattaccca aaggttaacg
     2281 agtttgaacg cctctgccca ctcgcccatc tctgatggtt tcccaagaac tcctcaagca
     2341 aaatatatat aattgtgtgt attatgcaca gacacgagaa aatgctgttt ttctgatctg
     2401 cattacagca catttgcccg ccaacgacaa taccacccac tcggtacctc gctgactcct
     2461 gatgcctgat acctgcgcgg tgactgtcta caatctgcat aatcaagaga agttgtgttg
     2521 aagacgagcg ccacacaacc gtttccacaa ggtcacccaa ggccggtgca gatgtaggtg
     2581 aggtctccat aaacagactg aaataaacac atcctccgct gggaacaaca accccctcac
     2641 gcctcatgca tttccataag cctacatgca tctcttccaa cttatggaga ctcgcaccta
     2701 ccaacatccg cacaacaaag atatacagag cgcgctccct caggtcaagg ccctgtgggg
     2761 gtctgtgcag aaataggtca tttgtcacac atcaagtcct ggggcaggag atgcattata
     2821 gatgagacca aacagcctgt ctcggtgagc tctacccact ccctgagact agaaatgggg
     2881 gaagggagct tgagataaca accgctgcaa tcactgtgtc gatgtttaat atcagcacca
     2941 accgggaaca ataaggagat gggtgcattc atgttcacat cttaccagtc aagtatcatc
     3001 gaaccggctt gataaccaca cctcgtgtaa tagctgagca gatagttgtc attttaaagc
     3061 gttggccttt gtcgattatg taatgcgcac attcaacaca tggtaatata gaaacggtta
     3121 tgtcgaggtt gttttgtcca gagatgacct tcacacagtt acagccgctc tgcatccaca
     3181 caaatggagg acttaatcgt ggactgcatt cttagaaatg atctacaaag acaaataatg
     3241 tgaaatcaag aaaggacaaa atttaagtaa ggggatgagg gagagagaga acgaggggca
     3301 aggagaaagc atggctcctg tctttttctg cacccatctg ttcggagtgc aggtggagct
     3361 ctattcactc agctctgcat gtgtgtttgg ggggggcagg aagaaaggga gggcaaaagg
     3421 aagagtggag agatggtggg ggctggaggg atggggggtt ctcggtgatc tctcctgaag
     3481 gggataatgg gagagcagcg ctttgcaatg gctgccatgt agtaccctcc ctgcacaatt
     3541 agccaatcag cagcaagctc tgccagccag aaggacacat aaaagaagaa cattgcagca
     3601 gaggcacaga aggagcctgc gaggagctgg gaaatacaca cacaacagca gaaccacaac
     3661 accctcccct ggacacaccc tactggggat cactgctttt ctttttttct gaaccatcgc
     3721 ccacgccaca cggagagaaa tctctctctc atcatcatcc tgaagaaaac ccccttatcc
     3781 tcattttcac actgctgagg aaaacctaca atcgcacggg ctgagatttc ctggcgaaga
     3841 ctgtcctttt tcctttttct ttttttttct tttcctttgg aaactgacat ttgcatttct
     3901 ccattccaag ccacggcgta ataatatctg caatccagcc tgaagacctg caaatcgaag
     3961 gacctagatc actatcatct ttgtacgtca aggatcctgg ttactgtacg tataaccttt
     4021 ctttcttttg ctctgaccaa tatgaaacac taaaatcgat tcgagcagcc tctcagcatc
     4081 aattacagtg cgtgaaaaac attcaaatag aggcagcaaa tattacatgt gaaaatacag
     4141 gctggctaaa tccaagctaa tttagaaatg tggtcaaaac gcatactggc acgtctaatc
     4201 gcggattcag taaacaagat taacgattag cccagtgtat aagtcatatg ataccggcat
     4261 gcgcgagagc atcgctacac ccgagctggc ttcattttcg gaggaaaatc aaaacattgc
     4321 tttctcctgc cgtgcgaacc attcgtcata aaccgtaata cgcaacatac atttattact
     4381 acatccgtta attagcgata attagccgtt attaacaaag agcgctgagg aattcctctc
     4441 aaatagcgga ggtgcggcgg aggcagaggg gcgtaaaagg gcacatgcgt gcctggctca
     4501 aaaaaggatg ctccagactg aaactcagag acaaaacacg atgcctcgga ggctgagagc
     4561 ccatcgagag gaaaggcaaa gaaaagggtc gagtcactca gagggggagg ggagatattg
     4621 tgcatccatt ggtttgaatt gaagcaggca gaataaaaat ccttgcgatt atgcttcgtg
     4681 agagatcgcg agagaggaaa aggctaaatg ccacgtatgc aaatggataa atgtcatcta
     4741 tttcttcgcg agtagacatt ttgtggacaa cgagagtgta aataagcgat atgaggggaa
     4801 tacaaaggcc ttgagggaaa gcgttttggg cgtgtcttgt cgaaaagaag acagcgatgg
     4861 cacgcgctca gaaccaccat cttcacgtag ataatccgcg tgaaaacaat aacaaaactg
     4921 ctcattttaa cacgagcagg tgtttgaaac cacccaaaaa agattgtcta aaatattaag
     4981 attaattaat gacgtttaaa tgtccaggcc tgatataaat gaagcgtgta gtaagagtaa
     5041 tcgcctttgt ttcttttcag agatacgttt tcagagattc agggaattat atcttcaata
     5101 aaataatgtt tgatttcatt attttaaagc tttttaagaa gtaaaaatac acattattca
     5161 tttacatttt attttattta aaggcctatg gtcttgaaaa taataagtgg aatatatagg
     5221 ctatatattc gtctaaggac agccggtgtt attttatagt gacctcagac aaaagccgag
     5281 acaaaacgag gcgcttgcct ttcattttaa tccttaatag acggtgtgat gaatgaatga
     5341 atgaatgaat aaatgaatga atgacgggcc cttcggcatg ttgttccgca gtcctccgtg
     5401 agcacgagtc tctaccgcag tgccaagcac ataaaaccgt tgagtcagat aaccttctcc
     5461 aggcgcgtat acacaattca aggacaagca catcacaact atacacaaca caacacaacc
     5521 gcctaaacgc acgagctcat caatcgcaat ttaaaggcat gtcagtcaaa gaaaaaggcg
     5581 gcgttattta aaaccaatgt tcaacaatat ttgtatgctg actagcccgg gcgcgcacgc
     5641 tataagatca caaggccttg ttttttccct ttattcaaaa tatcattaat tattacaggt
     5701 cataaaacaa aacaatgcga attacaatga tttaaagact attgatttaa caataatcag
     5761 ttaattatag aataatatta taatatttta catacaaatg aaagatgaca ttatttgatg
     5821 acattgtaat taaaatatat gcaatatatt tttacgtttt tattaattag gctattacta
     5881 ttattataat tatcattatt tttattatta ttattattat taaatagtct tgcgaaataa
     5941 gagtgaaggc agttgcaatg ttcattgttc ccggagagtc ccgcagcctc tggcctcaac
     6001 acaaagcgct actgtttaca ttaatattca tagtgctgcc atggtctctg tagaggagga
     6061 acgggtggct cttttgttcg ctagtgtcaa tacgtttatt cagcaaaaag cagctcggat
     6121 ttgtgaatgc ccaacaagca cacgcgcact ccaatcaaag gacgagagga taacgatttt
     6181 aaacgattcg caaacgtgtt tatttgtaaa gacgacatca agagcttaat gtccacttga
     6241 aaagaaataa atatgcccgc ggcgccaagg atattttgaa tgtagtcgtt catggtgagc
     6301 cactcccgaa ttcagcactc tggagagcga ccaaagagga atagcggaat agcaactact
     6361 tgagccattt ctctccgttt gatggcgttc atgattaaaa atataatcac gtgatttttt
     6421 atacatatat aaatatatac ataaagtaac gctttccttt tgagaataca taaatattta
     6481 ttcgcgtgat aaatcaatga tcttatattt atttgcacga ccgaaattag ccaaattcac
     6541 caattaaaaa aaaataagcc aacaaaaaag aagggtttat ccgttcaatt ttgacttgtg
     6601 tcctgttgtt ttacagctgc cactgtgatc ccttaagctg cagtgagagt gtggctaatg
     6661 ccttttgttt aagataatcc tgtaatctgt taccgaaacg gcctattgac aagccggcat
     6721 tcacatttca gtcaggaggc ccgtccagac atgtacattt atgaattacg aatgataaaa
     6781 ttaagatctg cattaggcgt cagaaatgtc acgaacaccc attcatttcc cacagccaat
     6841 aatggatcat gctgggaagc gctatcctcg cagtctcaaa attaatgttg acatgttgcg
     6901 tagagctaca gattagatca gccatatgct ttatgtgttt acctcagcgg atcttcagca
     6961 agatgtgttt attttaagaa aatggcttcc tcgctgcctg cacaggggca ttaaggaagt
     7021 gacgtgagcg tcctcacagt caaattactt tatctcatgc tgctttcagg cctctgctta
     7081 ttttattatt attattttaa attaggggga atcacggtgg cgttgtgggt agggtagcgc
     7141 gatcacctta cagcaagaag gtcgctggtt cgagctctgg ctgggtcagt tggcatttct
     7201 gtgtggagtt taaattgaat tgaaattgaa taaactaaat tggccctagt gtatgtgtgt
     7261 gaatgtaagt gtgcatgggt gtttcccagt gttgggtttg actggaagag catccgcttc
     7321 gtaaaacata tgctggataa gttggcggtt catttcactg tggtgaccgc tgattaataa
     7381 agggactaag ctgaaaatga aatgaattaa tacattttaa atgagctttt gctgacatgt
     7441 ttatgattac aaatagtgaa attgtatgtt aatttacgat aagtgcattg ttctaaattc
     7501 atttttatgt agcagctggt ttttcttcca tttgtgtcat tcaacagaac atttttctaa
     7561 aaaagtgaac ttaaattttc tcctccctct gacaaggctt gatggtaatt tcagccgact
     7621 ctaattacag actcactaaa aggaatcggt gcagtcattt atctttatca gcgcgctggt
     7681 gggaaggtaa taggttttgc ttatgagttg tttgcgtgcg agtctgggtc cttgtgtagg
     7741 cgatgctgat gcactgcatt aaatattgag ggagaagtcg ctcaaaaata gcaaagtaca
     7801 ggctggggca ggaacgtcaa agcccgctca gtggccacgt ttcttgtttc gtaagtccta
     7861 tttttaatag cgataattca ggctcagtag aaggaactcc agcactataa gcagtctctc
     7921 gcctgtctcg gcgagttatg tattttggga tgaagtgctc ttccacctct acctctgctg
     7981 tgctggcgcc agtatctggg ctgtaagata gtgtacgtgt cagtctgtgt ggccgaagag
     8041 cctgtgtgtt gtgtaactca ctacattcgt cgctgtggat ggctgacttg ccatggggag
     8101 gtttacttca gttcacgaat cacattcgcc acgatcttgt cactttgtgt caagactcgc
     8161 tctctgtgtg tcaacagctc tgctgtttga ggttgaaatt ctgaacttga gatatatccc
     8221 aggatatatt agggctactg ccaacggaaa aagcaaatgg tgccatggcc atttgattga
     8281 tcaaacaaac acccttttgc ttgggtgaca gtgaaatgtc agatccatgt ttgtactgct
     8341 ttaataactt gcctctctga gctttctcct tgtcagtgtc aatttgcctg agatataaat
     8401 tccggttgcc ccgaaagctt attttattaa aaaaaagcta acatgcactc atacaacgtg
     8461 cgcacataca tgtgaggctt gctaaaagtc aagaagcaag ccaatagcaa gagtcgtcag
     8521 actgctgtta cctgggcaac ctggttagag gtaaggctca aggcaagaat aaatcattcc
     8581 actgaagaga cacagcgtta cagcgagatg agagcaagag gcgatagtga agggagaaca
     8641 aaagaacagc ctacccacac tagtgcagat ctacctgttt gagctactga ttgttcagtt
     8701 tgattagata tcagacaatt ctgctatctc caattgcatt gctgcatgaa ttcctagaaa
     8761 tcattttaat caccaaaaac tagtagggac acttgtggcg tatatgattc tgcctgttga
     8821 ttgtggggat ccaacagaaa gatcatgaat gcattgttaa ttaagtcggt gagacaccct
     8881 gttccaccct aggggccgtt ctggggaaag agtgctttca gtcagctaaa gatgactaat
     8941 atgtgaacat atattactac acatgccaat ttgtacattc tggatagtta ccagccctgg
     9001 gaaaacatcg aaaaaaataa atcaacatag ataaaattgg caaatccctt ctggtacgat
     9061 gcagagtgct tgctctgaga acttgtgcat tgtgacagaa agcgaccgtt aaaaactgaa
     9121 agtagcatct acaacctgca tacatctgtt ctttgaaaat cccctgtagg ccaaattaag
     9181 catcacgctg tgctccacag caacacggat gaaccgaaaa cccagacaca aacgcacaca
     9241 cttttcacat aaacaaggtt tagttatgct agctatgctt tttcgtcctc cctttgtcat
     9301 cagcggcgag tgacgtaaca cagtttgacc ctggacagcg taaagggggg gttgggaatg
     9361 agtgaaagga ctgtgacatc tgcagggcca ggggctgatg gagagagctg aagtgaggga
     9421 ggtcagagga ggaggaaggg tgggatattg tcctcctcag gccctatccc tctcagacgc
     9481 attgattttt aacctctcag cgagaatgcc aagcattaca cccctcaaat tatggttcct
     9541 cacccaacct gttatatttt ccacctgcag cacccagctt tcttgtacaa agtggtcact
     9601 agtcaccatg ggggagaaac ccgggaccag ggtcttcaag aagtcgagcc ctaactgcaa
     9661 gctcaccgtg tacttgggca agcgggactt cgtagatcac ctggacaaag tggaccctgt
     9721 agatggcgtg gtgcttgtgg accctgacta cctgaaggac cgcaaagtgt ttgtgaccct
     9781 cacctgcgcc ttccgctatg gccgtgaaga cctggatgtg ctgggcttgt ccttccgcaa
     9841 agacctgttc atcgccacct accaggcctt ccccccggtg cccaacccac cccggccccc
     9901 cacccgcctg caggaccggc tgctgaggaa gctgggccag catgcccacc ccttcttctt
     9961 caccataccc cagaatcttc catgctccgt cacactgcag ccaggcccag aggatacagg
    10021 aaaggcctgc ggcgtagact ttgagattcg agccttctgt gctaaatcac tagaagagaa
    10081 aagccacaaa aggaactctg tgcggctggt gatccgaaag gtgcagttcg ccccggagaa
    10141 acccggcccc cagccttcag ccgaaaccac acgccacttc ctcatgtctg accggtccct
    10201 gcacctcgag gcttccctgg acaaggagct gtactaccat ggggagcccc tcaatgtaaa
    10261 tgtccacgtc accaacaact ccaccaagac cgtcaagaag atcaaagtct ctgtgagaca
    10321 gtacgccgac atctgcctct tcagcaccgc ccagtacaag tgtcctgtgg ctcaactcga
    10381 acaagatgac caggtatctc ccagctccac attctgtaag gtgtacacca taaccccact
    10441 gctcagtgac aaccgggaga agcggggtct cgccctggat gggaaactca agcacgagga
    10501 caccaacctg gcttccagca ccatcgtgaa ggagggtgcc aacaaggagg tgctgggaat
    10561 cctggtgtcc tacagggtca aggtgaagct ggtggtgtct cgaggcgggg atgtctctgt
    10621 ggagctgcct tttgttctta tgcaccccaa gccccacgac cacatccccc tccccagacc
    10681 ccagtcagcc gctccggaga cagatgtccc tgtggacacc aacctcattg aatttgatac
    10741 caactatgcc acagatgatg acattgtgtt tgaggacttt gcccggcttc ggctgaaggg
    10801 gatgaaggat gacgactatg atgatcaact ctgcggatct agcttgttta agggaccacg
    10861 tgattacaac ccgatatcga gcaccatttg tcatttgacg aatgaatctg atgggcacac
    10921 aacatcgttg tatggtattg gatttggtcc cttcatcatt acaaacaagc acttgtttag
    10981 aagaaataat ggaacactgt tggtccaatc actacatggt gtattcaagg tcaagaacac
    11041 cacgactttg caacaacacc tcattgatgg gagggacatg ataattattc gcatgcctaa
    11101 ggatttccca ccatttcctc aaaagctgaa atttagagag ccacaaaggg aagagcgcat
    11161 atgtcttgtg acaaccaact tccaaactaa gagcatgtct agcatggtgt cagacactag
    11221 ttgcacattc ccttcatctg atggcatatt ctggaagcat tggattcaaa ccaaggatgg
    11281 gcagtgtggc agtccattag tatcaactag agatgggttc attgttggta tacactcagc
    11341 atcgaatttc accaacacaa acaattattt cacaagcgtg ccgaaaaact tcatggaatt
    11401 gttgacaagt caggaggcgc agcagtgggt tagtggttgg cgattaaatg ctgactcagt
    11461 attgtggggg ggccataaag ttttcatgag caaacctgaa gagccttttc agccagttaa
    11521 ggaagcgact caactcatga atgaattggt gtactcgtga ccgcggtgga gctcgaatta
    11581 attcatcgat gatgatccag acatgataag atacattgat gagtttggac aaaccacaac
    11641 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
    11701 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
    11761 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
    11821 ggctgattat gatcctctag atcagatcta atctgatcat tcaggcgcgc ctcaaccact
    11881 ccaggcatag ctagagccac tgatgcatct atagagctga agtaaagata gggttgatgc
    11941 aaatcgattt agaattttca atgtagaaaa aacaaaataa aaagtctatt cacttgatgg
    12001 atagattagt caaacaaaca tagagtaggc acttcccttg actctgtaaa cttttggatt
    12061 taacagattg tgcactcctt ttagttaaac acattagtcc ctgaaaacta agaccctaac
    12121 cataatccca ttgtagaccc acctactcgg gtgaatataa agctcgcaca gatcatctca
    12181 cagacatcag accttggcaa aggagcatcc tgaacatctc tacacactga ggcaacccta
    12241 gggccgccac catgctgtgt tgtatcagaa gaacaaagcc tgtggagaag aatgaggagg
    12301 ctgaccagga actgcagagc acggtaccta gagctagaga ccctcctgtg gctacaatgg
    12361 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
    12421 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
    12481 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
    12541 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
    12601 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
    12661 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
    12721 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
    12781 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
    12841 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
    12901 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
    12961 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
    13021 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtat aagtgagaat
    13081 tctgcaggaa ttgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
    13141 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
    13201 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
    13261 gttgggaaga caacctgtag ggcacgaggg ggggcccgaa acccgctgat cagcctcgac
    13321 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
    13381 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
    13441 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
    13501 ggaagacaat agcaaggcat gctggggatg cggtgggctc tatggacgcg tgatctgcga
    13561 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
    13621 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
    13681 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
    13741 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
    13801 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
    13861 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
    13921 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
    13981 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
    14041 caagtaaaat tactcaagta ctttacacct ctggttcttg aggtacctct tccgcttcct
    14101 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    14161 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    14221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    14281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    14341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    14401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    14461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    14521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    14581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    14641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    14701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    14761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    14821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    14881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    14941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    15001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    15061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    15121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    15181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    15241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    15301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    15361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    15421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    15481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    15541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    15601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    15661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    15721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    15781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    15841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcat
//
