LOCUS       sequence-466497-       10525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to introduce an OsTir1(F74G) expressing cassette at
            the mouse Rosa26 locus using Neomycin selection. AID2 degron system.
            Use with Addgene #64216 sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Lucero K, Han S, Huang P, Qiu X, Mazzoni EO, Reinberg D
  TITLE     CTCF-RNA interactions orchestrate cell-specific chromatin loop 
            organization
  JOURNAL   bioRxiv, March 19, 2025
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 19, 2025"
COMMENT     Sequence Label: KL063 -
            pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-Ne...
FEATURES             Location/Qualifiers
     source          1..10525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1599..1621
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1761..3491
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKILR
                     "
     primer_bind     complement(3579..3598)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3644..3699
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3698..3717)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4060..4076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4084..4100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4108..4114,4115..4132,4133..4138))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4153..4174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4238..4271
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        4335..4834
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4370..4393)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4767..4786
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        4846..4893
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4912..5715
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4975..4994)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5585..5604
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5747..5764)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5753..5977
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6032..6065
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        complement(6891..6909)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6930..6946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6954..6970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6978..6984,6985..7002,7003..7008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7023..7044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7161..7178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7332..7920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7412..7431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8091..8882,8883..8951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8714..8733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8952..9056)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9082..9537)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9219..9240)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9431..9450
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9664..9686
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9678..9695
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9679..9695
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9702..9721
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9702..9720
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggggggcccc gggggggggg gggggggggg gggggggggg
      541 gggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc tcgagaattc
     1741 tcacgcgtct gcaggatatc atgacatact ttcctgaaga ggtcgtcgaa cacattttta
     1801 gcttcctgcc tgcacagaga gatagaaaca cagtgagcct ggtctgcaaa gtgtggtacg
     1861 agatcgaacg cctgagccgg agaggagtgt tcgtcggcaa ctgctatgct gtgagagcag
     1921 gcagggtcgc cgctaggttt ccaaatgtgc gcgcactgac cgtcaagggg aaaccccacg
     1981 gcgccgactt taacctggtg ccccctgatt ggggaggata cgccggccct tggatcgagg
     2041 cagccgctcg cggctgtcat ggactggagg aactgcgcat gaagcgaatg gtggtctctg
     2101 acgaaagtct ggagctgctg gctcggagct tccctaggtt tcgcgcactg gtgctgattt
     2161 cttgcgaagg cttcagcacc gatggactgg cagccgtggc ctcccactgt aagctgctgc
     2221 gggagctgga cctccaggag aatgaagtgg aggatagagg ccccagatgg ctgtcttgct
     2281 tcccagactc atgtaccagc ctggtgtccc tgaactttgc ctgcatcaaa ggcgaagtga
     2341 atgctgggtc cctggagcgg ctggtctcaa gaagccccaa cctgaggtct ctgcggctga
     2401 accggagcgt gagcgtggac actctggcta agattctgct gagaacccct aacctggagg
     2461 atctgggaac cggcaatctg acagacgatt tccagacaga atcctacttt aaactgactt
     2521 ctgccctgga gaagtgtaaa atgctgagga gtctgtcagg attctgggat gcttcacccg
     2581 tgtgcctgag ctttatctac cctctgtgtg cacagctgac aggcctgaac ctgagctatg
     2641 caccaaccct ggacgccagt gatctgacaa agatgatctc acgctgcgtg aaactccagc
     2701 gactgtgggt gctggactgt atttccgata aggggctcca ggtggtcgcc agctcctgca
     2761 aggacctcca ggagctgaga gtgttcccat ctgattttta cgtggccgga tatagtgctg
     2821 tcactgagga aggcctggtg gcagtctcac tgggatgccc aaagctgaac agcctgctgt
     2881 atttctgtca tcagatgact aatgctgcac tggtgaccgt cgccaagaac tgccctaatt
     2941 tcacccgatt tcggctgtgt attctggaac caggcaaacc cgacgtggtc acatcccagc
     3001 cactggatga agggtttgga gctatcgtga gagagtgcaa gggactccag aggctgagca
     3061 tttccggcct gctgacagac aaagtgttca tgtacatcgg caagtatgct aagcagctgg
     3121 agatgctgag cattgcattt gccggagact ccgataaggg catgatgcac gtgatgaacg
     3181 ggtgtaagaa tctgcgaaaa ctggaaatcc gggacagccc tttcggggat gccgctctgc
     3241 tgggaaactt tgccagatac gagacaatga ggagcctgtg gatgtctagt tgcaatgtga
     3301 ctctgaaggg ctgtcaggtc ctggctagta aaatgcctat gctgaacgtg gaagtcatta
     3361 atgagcggga cgggtctaac gaaatggagg aaaatcatgg cgacctgcca aaggtggaga
     3421 aactgtatgt gtatcggacc accgcagggg caagagatga tgctcccaac tttgtgaaga
     3481 ttctgagatg acccgggtaa gcggcctcag gtgcaggctg cctatcagaa ggtggtggct
     3541 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     3601 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     3661 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     3721 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     3781 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     3841 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     3901 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     3961 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     4021 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4081 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4141 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4201 ccagtcggga aacctgtcgt gccagcggat caattccgaa gttcctattc tctagaaagt
     4261 ataggaactt caggtctgaa gaggagttta cgtccagcca agctagcttg gctgcaggtc
     4321 gtcgaaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     4381 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     4441 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     4501 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     4561 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     4621 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     4681 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     4741 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     4801 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcctgttga caattaatca
     4861 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgggatcg
     4921 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4981 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5041 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5101 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5161 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5221 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5281 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5341 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5401 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5461 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5521 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5581 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5641 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5701 gacgagttct tctgagggga tcaattctct agagctcgct gatcagcctc gactgtgcct
     5761 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5821 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5881 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5941 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6001 tggggctcga ctagagcttg cggaaccctt cgaagttcct attctctaga aagtatagga
     6061 acttcatcag tcaggtacat aatggatccg gaagaggggg aagggattct cccaggccca
     6121 gggcggtcct cagaagccag gaggcagcag agaactccca gaaaggtatt gcaacactcc
     6181 cctcccccct ccggagaagg gtgcggcctt ctccccgcct actccactgc agctccctta
     6241 ctgataacaa ctcagagcga ctttgggaga gcaagtgctt cctgcctcca aaacagccca
     6301 actgagccct cgctccttcc ctccactccc cggagtgcgc gatggaggtc tggctcagca
     6361 cgcccctctt gaggcaactc aagtcggaaa cgtgcttgca cccgccccgc agccgctcag
     6421 ccctactgcc cgtccccgcc cccagcgcgc gcttcctgcc acgttgcgca ggggcgcgcg
     6481 gccagactct gcggcgcggg gccgagggga gggccggaac ctgggagcgc ctcctcgccg
     6541 cccccgctgg ccggcggatg gactcaactt gcacgaacac gagccaatgg caagggccag
     6601 ttttctgggc cccgagagcc aatcagacga cgaggcccgg ccggcggcgg gtaaaacgac
     6661 tcccccagag gaaggggagg gtgggcggcc gctcgcgcgc gagctacttt cgctgaccct
     6721 ccctcccctc ccccgccccg ccagaggccg accgcgcccg cacgtccagc tcgcctcacc
     6781 ccacctacct cccgccccac ccagtgggca gagcgaggct gccggcggct gcgcactccg
     6841 gctgccgtta actggcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     6901 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6961 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7021 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7081 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7141 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7741 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8401 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8701 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9061 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9121 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9181 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9241 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9301 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9361 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9421 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9481 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     9541 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9601 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9661 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     9721 gcgaattggg taccataaat ggagaaaaag gagagaggca ttcatgggag tggaaagtta
     9781 agccttaggt tggattctca atacatctat tgttgtaaaa ctccctggac tgagaatagg
     9841 cccaaatgtg gaacaccacc tgacgggaga ggtgatagac actgaaaatt aaggatcaag
     9901 gcaaaggatc aacaaaaagt gtactaagga gttataaaag aactgcagtg tcgaggcccc
     9961 agctacagcc tcgatttgtg gtgtatgtaa ctaatctgtc tggtttcatg agtcatcaga
    10021 cttctaagat caggaaaggg aaaatgccaa tgctctgtct aggggttgga taagccagtc
    10081 taataaatga aaatggggct aaaatgagtg ttctaaaata ccttttgata aggctgcaga
    10141 aggagcggga gaaatggata tgaagtactg ggctctttaa aaatgattaa aattctgctt
    10201 acatagtcta actcgcgaca ctgtaatttc atactgtagt aaggatctca agcaggagag
    10261 tataaaactc gggtgagcat gtctttaatc tacctcgatg gaaaatactc cgaggcggat
    10321 cacaagcaat aataacctgt agttttgctg cataaaaccc cagatgacta cctatcctcc
    10381 cattttcctt atttgcccct attaaaaaac ttcccgacaa aaccgaaaat ctgtgggaag
    10441 tcttgtccct ccaattttac acctgttcaa ttcccctgca ggacaacgcc cacacaccag
    10501 gttagccttt aagcctctcg aggtc
//
