LOCUS       sequence-466502-       13579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce a constitutive rtta3G cassette and a 
            DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor
            locus. Use with Addgene #92144 sgRNA. Use puromycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13579)
  AUTHORS   Lucero K, Han S, Huang P, Qiu X, Mazzoni EO, Reinberg D
  TITLE     CTCF-RNA interactions orchestrate cell-specific chromatin loop 
            organization
  JOURNAL   bioRxiv, March 19, 2025
REFERENCE   2  (bases 1 to 13579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 19, 2025"
COMMENT     Sequence Label: KL094 -
            pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-r...
FEATURES             Location/Qualifiers
     source          1..13579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1380..1729
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1388..1406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1424..1442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1460..1478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1496..1514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1532..1550
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1568..1586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1604..1622
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1668..1692
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1829..1838
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1838..1861
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1868..2752
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             2774..2794
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     polyA_signal    5039..5263
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5296..5431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5536..5557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5572..5577,5578..5595,5596..5602)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5610..5626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5634..5650
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5993..6012
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6011..6066
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6112..6131
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6210..6956)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKGKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7053..7072)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          7080..8096
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7134..7156)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        8374..8677
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8741..8774
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8873..8896)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9246..9265
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9325..9372
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9391..9990
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9391..9410)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9887..9907
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10028..10252
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    10307..10340
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11358..11374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11382..11398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11406..11412,11413..11430,11431..11436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11451..11472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11589..11606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11760..12348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11840..11859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12519..13310,13311..13379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13142..13161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13380..13484)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctgcaggg ggtatcacga ggccctttcg tcttcactcg
     1381 agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga
     1441 gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag tttactccct
     1501 atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac
     1561 agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga
     1621 gaacgtatgt cgaggtaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
     1681 aaccgtcaga tcgcctggag caattccaca acacttttgt cttatactta cgcgtctgca
     1741 ggatatcaag cttgcggtac cgcggccccg aattcgagct cggtacccgg ggatcctcta
     1801 gtcagctgac gcgtgctagc gcggccgcgc caccatggac tacaaggacg atgatgacaa
     1861 gcccggggaa atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga
     1921 gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg
     1981 taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg
     2041 ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt
     2101 cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt
     2161 cgtcctggtc attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc
     2221 agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga
     2281 atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa
     2341 gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc
     2401 gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga
     2461 gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc
     2521 gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg
     2581 gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc
     2641 taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga
     2701 cctgatcggc agcgagatcg cgcgctggct gtctactctg gagatttccg gtcttaaggg
     2761 gggagcagga ggcgaggatc tgtactttca gagcgataac gatggatcca tggaaggtga
     2821 ggcggttgaa gccattgtgg aggagtctga aactttcatt aaaggaaaag aaagaaagac
     2881 ttaccagaga cgccgggaag ggggccagga agaagatgct tgccacctgc cccagaacca
     2941 gacagatggg ggtgaggtgg tccaggatgt caacagcagt gtacagatgg taatgatgga
     3001 acagctggat cctacccttc tccagatgaa gactgaagtc atggagggta cagtggctcc
     3061 ggaagcagag gctgcagtgg acgataccca gatcataacc ttgcaggttg taaatatgga
     3121 ggaacagccc attaacatag gagagcttca gcttgtccaa gtacctgttc ctgtgacggt
     3181 acctgttgct actacttcag tagaagaact tcagggggct tatgagaatg aagtgtctaa
     3241 agagggcctt gcagaaagtg aaccgatgat atgtcacacc ttacctttgc ctgaaggatt
     3301 tcaggtggtg aaagtggggg ccaatggaga agtggagaca ctagagcagg gcgagcttcc
     3361 tcctcaggaa gactctagct ggcaaaaaga cccagactat cagccaccag ccaaaaaaac
     3421 aaagaaaacc aaaaagagca aacttcgtta cacagaagag ggcaaagacg tggatgtgtc
     3481 tgtgtatgat tttgaggaag aacagcagga aggactgctg tctgaggtta atgcagagaa
     3541 agtagttggt aatatgaagc ctccgaagcc aacaaaaatt aaaaaaaaag gtgtaaagaa
     3601 aacattccag tgtgagcttt gcagttacac atgtccccgg cgttcaaatt tggatcgtca
     3661 catgaaaagc cacactgatg agagaccaca caaatgccat ctgtgtggca gagcattcag
     3721 aacagtgacc ctcctgagga atcatctgaa cacacacaca ggtactcgtc ctcacaagtg
     3781 cccagactgc gatatggcct ttgtgaccag tggagaattg gtgcggcatc gtcgttataa
     3841 acacactcat gagaaaccat ttaagtgttc catgtgtgat tatgccagtg tagaagtcag
     3901 caaattaaaa cgacacattc gctctcatac tggagagcgc ccgttccagt gcagtttgtg
     3961 cagttatgcc agcagggaca catacaagct gaaaaggcat atgagaaccc attcagggga
     4021 aaaaccttat gaatgttata tttgtcacgc tcggtttacc cagagtggta ccatgaagat
     4081 gcacatttta cagaagcaca cagaaaatgt ggccaaattt cattgtcccc attgtgacac
     4141 tgtcatagcc cgaaaaagtg atttgggtgt ccacttgcga aagcagcatt cctatattga
     4201 acagggcaaa aaatgtcgct actgtgatgc tgtgtttcat gagcgatatg ctctcatcca
     4261 gcatcaaaaa tcacacaaaa atgagaagcg cttcaagtgt gaccagtgtg attatgcttg
     4321 tagacaggag cggcacatga tcatgcacaa gcgcactcac acgggggaga agccttatgc
     4381 ctgcagccac tgcgacaaga ccttccgcca gaaacagctc ctcgacatgc atttcaagcg
     4441 ctatcatgat cccaactttg tccctgctgc ctttgtctgt tccaagtgtg ggaaaacatt
     4501 cacccgccgg aacacaatgg caagacatgc agataactgt gctggtccag atggcgtaga
     4561 gggggaaaat ggaggggaga caaagaagag caaacgagga agaaaaagaa agatgcgatc
     4621 taaaaaagaa gactcctctg acagtgaaga aaatgctgag ccggatctcg atgacaatga
     4681 ggaggaggag gagcctgctg tagaaattga acctgagcca gagcctcagc cccagcctcc
     4741 gcctccacct cagcctgtgg ccccggcccc cccacctgcc aagaagagaa gggggcgacc
     4801 tcctggaaga accaaccagc ccaaacagaa ccagccaaca gccatcattc aggtcgaaga
     4861 tcagaataca ggtgcaattg agaacattat agttgaagtc aaaaaggagc cagatgccga
     4921 gcctgcggag ggggaagaag aggaggctca ggcagccacc acagacgccc ccaacggaga
     4981 cctcacgcct gagatgatcc tcagcatgat ggaccggtga gtttaaacat agcctcgact
     5041 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5101 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5161 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5221 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5281 accttaatta acctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     5341 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     5401 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
     5461 ctgactaatt gagatgcgga tccgctggca cgacaggttt cccgactgga aagcgggcag
     5521 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5581 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     5641 cagctatgac catgattacg ccaagcttgg gctgcaggtc gagggatctc cataagagaa
     5701 gagggacagc tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat
     5761 gtaaggaaaa ttttagggat gttaaagaaa aaaataacac aaaacaaaat ataaaaaaaa
     5821 tctaacctca agtcaaggct tttctatgga ataaggaatg gacagcaggg ggctgtttca
     5881 tatactgatg acctctttat agccaacctt tgttcatggc agccagcata tgggcatatg
     5941 ttgccaaact ctaaaccaaa tactcattct gatgttttaa atgatttgcc ctcccatatg
     6001 tccttccgag tgagagacac aaaaaattcc aacacactat tgcaatgaaa ataaatttcc
     6061 tttattagcc agaagtcaga tgctcaaggg gcttcatgat gtccccataa tttttggcag
     6121 agggaaaaag atctcagtgg tatttgtgag ccagggcatt ggccacacca gccaccacct
     6181 tctgataggc agcctgcacc tgaggccgct tacccgggga gcatgtcaag gtcaaaatcg
     6241 tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc
     6301 atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt
     6361 ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc
     6421 cggtcgaaca gctcaattgc ttgtttcaga agtgggggca tagaatcggt ggtaggtgtc
     6481 tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag
     6541 tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg
     6601 aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc
     6661 actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc
     6721 agaaagtctt gccatgactc gccttccagg gggcaggagt gggtatgatg cctgtccagc
     6781 atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta
     6841 ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgact
     6901 ccattgagta attccagagc agagtttatg actttgccct tgtccagtct agacatggtg
     6961 gcggcggatc ccgggcccgc ggtaccgcaa gcttgatatc ctgcagacgc gtgagaattc
     7021 tcgaggaatt ctttgccaaa atgatgagac agcacaataa ccagcacgtt gcccaggagc
     7081 tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc gccggtcaca
     7141 cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc cccccgcgga
     7201 cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg aaggccctcc
     7261 ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct agagggggtg
     7321 cggcggcgcc tcccagattt cggctccgca cagatttggg acaaaggaag tccctgcgcc
     7381 ctctcgcacg attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg acagccgccg
     7441 gcgctccggg ggccgccgcg ccccyccccc gagcccyccc cggcccgmgg cggccccgcc
     7501 ccgcccggca cccccacctg ccgccacccc ccgcccggca cggcgagccc cgcgccacgc
     7561 cccgtacgga gccccgcacc cgaagccggg ccgtgctcag caactcgggg aggggggtgc
     7621 agggggggtt gcagcccgac cgacgcgccc acaccccctg ctcacccccc cacgcacaca
     7681 ccccgcacgc agcctttgtt cccctcgcag ccccccccgc accgcggggc accgcccccg
     7741 gccgcgctcc cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc
     7801 tcacagccgc cgggcagcgc ggagccgcac gcggcgctcc ccacgcacac acacacgcac
     7861 gcaccccccg agccgctccc cccgcacaaa gggccctccc ggagcccctc aaggctttca
     7921 cgcagccaca gaaaagaaac aagccgtcat taaaccaagc gctaattaca gcccggagga
     7981 gaagggccgt cccgcccgct cacctgtggg agtaacgcgg tcagtcagag ccggggcggg
     8041 cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc gactcccgcc
     8101 cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac
     8161 tttcggagcg cgccgctctg attggctgcc gccgcmccyc yccsccycsc cccscccccc
     8221 cccccscccc cccccccccc cccyggcgcg cscccccccc ccccccgccc ccatcgctgc
     8281 acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg
     8341 gagagtgaag cagaacgtgg ggctcacctc gaccatggta atagcgatga ctaatacgta
     8401 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     8461 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     8521 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     8581 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     8641 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     8701 aactaatgac cccgtaattg attactatta ataactagtc gaagttccta ttctctagaa
     8761 agtataggaa cttcaggtct gaagaggagt ttacgtccag ccaagctagc ttggctgcag
     8821 gtcgtcgaaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     8881 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     8941 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     9001 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     9061 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     9121 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     9181 tcagaggctg ggaaggggtg ggtccggggg cggggcgggc gcccgaaggt cctccggagg
     9241 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     9301 tccgggcctt tcgacctgca gcctgttgac aattaatcat cggcatagta tatcggcata
     9361 gtataatacg acaaggtgag gaactaaacc atgaccgagt acaagcccac ggtgcgcctc
     9421 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     9481 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     9541 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     9601 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     9661 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     9721 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     9781 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     9841 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     9901 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     9961 tggtgcatga cccgcaagcc cggtgcctga ggggatcaat tctctagagc tcgctgatca
    10021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    10081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    10141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    10201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
    10261 gcggaaagaa ccagctgggg ctcgactaga gcttgcggaa cccttcgaag ttcctattct
    10321 ctagaaagta taggaacttc ggcgcgccat ttaaattttg ggatgttttc tgtttgtttt
    10381 gagatagggt ttttcagtgt agtcctggct gtcctagaac tcactctgta gaccaggctg
    10441 gcctggaact caaggactgc cagcctctgc ctcctgagca tcgggattaa aggtgtgtgc
    10501 caccactgcc cagcagagtg cgtttttact cttcactttg tgggtcctgg ctgcctgaga
    10561 ttgtgtgctt gcaccattag atgggaggga tgacttggga aagggttgag tcttttgtga
    10621 gtcttgcgga ggcccaggag ggggtacgta aattagagtt ctaagaggcc cagcaaaagt
    10681 gaacaagtga ctggccactg tgagttcact gggggacctg agagttaact agtgaaaagg
    10741 agaggtccta gtggagaagc taaagaatta aagttactgc cttgaagttc tttgtgaagg
    10801 ccaagctctg gagcgcggct tgaaaagcct ttatcccaga gcagcatggg aaggggcccc
    10861 agctgggtgc gagcctgcaa accagagcac acctatcaca gggccttttc ttaccagtgg
    10921 gacgggatca aggcggagct tacctgggaa cgtacagggc ggaaccttaa cttgggggcg
    10981 tggccgaatc acctgggttt ggaaacggtt cttgtctttc ctcaagctca ggagaggttg
    11041 tttgcaggac actggcccgg aggggcccga actctccgag ggagggtttc gtggatcaga
    11101 acaccgtccc ttgacaaaaa gatgcagagc accgtctgca ttctgctagt aaccgtccct
    11161 cctgggggcg gagccagccc tcctccgggt gtataaagta gctgagcgcc tccttctgac
    11221 aaacgcctca cccccaagag tactggggga gtacgcagca cgccagccta ggggcggagc
    11281 tcagccctgg aggaggcgtt tcttcttagt tctccgactc acgcctctcc acttttcatt
    11341 taaatggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    11401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    11461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    11521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    11581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    11641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    11701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    12001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    12061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    12121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    12181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    12241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    12301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    12361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    12421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    12481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    12541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    13021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    13081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    13141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    13201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    13261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    13321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    13381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    13441 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    13501 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    13561 atcacgaggc cctttcgtc
//
