LOCUS       sequence-466504-       10605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LbNOX in human cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Choe M, Ekvik AE, Stalnaker G, Shin HR, Titov DV
  TITLE     Genetically encoded tool for manipulation of DeltaPsim identifies 
            its role as the driver of ISR induced by ATP synthase dysfunction.
  JOURNAL   Cell Chem Biol. 2025 Apr 17;32(4):620-630.e6. doi: 
            10.1016/j.chembiol.2025.03.007.
  PUBMED    40250406
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2025.03"; journalName: "Cell Chem Biol"; date: 
            "2025-04-17- 17"; volume: "32"; issue: "4"; pages: "620-630.e6"
COMMENT     Sequence Label: pLVX-TetOne-Puro-LbNOX-Flag
FEATURES             Location/Qualifiers
     source          1..10605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2561..3160)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2644..2664)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3141..3160
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3169..3498)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3183..3318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(3509..4255)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(4274..4784)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4337..4355)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4801..5168
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    4809..4827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4845..4863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4881..4899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4917..4935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4953..4971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4989..5007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5025..5043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      5175..5184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6567..6590
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6800..6934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6978..7093
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(7356..7400)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7585..7818
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8315..8440
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8487..9120
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9288..9422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9470..9574
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9575..9643,9644..10435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9793..9812)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgt tcaggcaccg ggcttgcggg
     2581 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     2641 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     2701 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     2761 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     2821 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     2881 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     2941 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     3001 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc
     3061 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt
     3121 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtcgcgttt tgcaaaagcc
     3181 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     3241 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     3301 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     3361 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     3421 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     3481 acaccctaac tgacacacat tcgcgcgttt acccggggag catgtcaagg tcaaaatcgt
     3541 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     3601 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     3661 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     3721 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     3781 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     3841 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     3901 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     3961 ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     4021 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     4081 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     4141 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     4201 cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta gacatggtaa
     4261 ttcgatgatc cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
     4321 acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca
     4381 ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc
     4441 ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct
     4501 ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc
     4561 acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg
     4621 ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc
     4681 gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct
     4741 gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttatcacctc
     4801 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4861 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4921 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4981 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     5041 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     5101 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     5161 ctcgtaaaga attcgccacc atggggatga aggtcaccgt ggtcggatgc acccatgccg
     5221 gcaccttcgc catcaagcaa atcctcgctg agcaccctga cgccgaggtc accgtctacg
     5281 agaggaacga tgtgatctcc ttcctgtcct gtggcatcgc cctctacctg ggcggaaaag
     5341 tggccgatcc ccaaggcctc ttctacagct cccctgaaga actgcagaag ctgggcgcta
     5401 atgtgcagat gaaccacaac gtgctggcca tcgaccctga ccaaaagacc gtcacagtcg
     5461 aggacctcac caatcacgcc cagaccaccg agtcctacga caaactggtg atgacctccg
     5521 gaagctggcc tatcgtgccc aaaatccccg gcatcgacag cgatagggtg aagctctgca
     5581 agaattgggc ccacgcccag gctctgattg aggacgccaa ggaggccaag aggatcaccg
     5641 tcatcggcgc cggatacatc ggagccgaac tggccgaggc ctactccaca acaggccacg
     5701 acgtcaccct gattgacgcc atggctaggg tcatgcccaa gtacttcgat gccgacttca
     5761 ccgacgtcat cgaacaggac tacagggacc atggcgtgca actcgctctg ggcgagacag
     5821 tggagagctt caccgacagc gccaccggcc tcacaatcaa gacagacaag aactcctatg
     5881 agaccgacct ggccatcctc tgcattggct ttaggcccaa cacagacctg ctgaaaggca
     5941 aagtggacat ggcccctaac ggcgccatca ttaccgacga ctacatgagg tccagcaacc
     6001 ctgatatttt cgctgctggc gactccgccg ccgtccatta caaccccaca caccaaaacg
     6061 cctacattcc cctcgctacc aacgccgtca ggcagggaat cctcgtcgga aagaacctcg
     6121 tcaagcccac agtgaagtac atgggaaccc agtccagctc cggactggcc ctctatgaca
     6181 ggacaattgt ctccacaggc ctcacactgg ccgccgccaa gcaacaaggc ctcaatgccg
     6241 agcaggtcat cgtggaggac aactataggc ccgagttcat gccttccacc gagcccgtcc
     6301 tcatgagcct ggtcttcgac cccgatacac acagaatcct gggaggcgcc ctgatgtcca
     6361 aatacgacgt gtcccagagc gctaataccc tgtccgtctg catccagaac gagaacacca
     6421 tcgatgacct ggccatggtg gacatgctgt tccagcccaa tttcgacagg cccttcaact
     6481 acctgaacat tctcgcccag gctgcccaag ctaaagtggc ccaatccgtc aacgctggtg
     6541 gatctggtgg atctggtgga tctatggatt acaaggatga cgatgacaag taagaattcc
     6601 cgtatacacc ggtgccggcg gatccagacc acctcccctg cgagctaagc tggacagcca
     6661 atgacgggta agagagtgac atttttcact aacctaagac aggagggccg tcagagctac
     6721 tgcctaatcc aaagacgggt aaaagtgata aaaatgtatc actccaacct aagacaggcg
     6781 cagcttccga gggatttgag atccagacat gataagatac attgatgagt ttggacaaac
     6841 caaaactaga atgcagtgaa aaaaatgcct tatttgtgaa atttgtgatg ctattgcctt
     6901 atttgtaacc attataagct gcaataaaca agttaagtcg ataaactgga tctctgctgt
     6961 ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt
     7021 gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc
     7081 cttttctttt aaaggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     7141 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     7201 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     7261 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     7321 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     7381 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     7441 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     7501 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     7561 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     7621 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     7681 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     7741 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     7801 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     7861 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     7921 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt
     7981 gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg
     8041 tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg
     8101 atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac
     8161 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
     8221 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
     8281 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
     8341 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
     8401 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
     8461 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
     8521 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     8581 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     8641 tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg
     8701 atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag
     8761 cggaaagtcc cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct
     8821 ctcgggccac gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct
     8881 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat
     8941 tggcctcttc taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca
     9001 gtggatatct gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg
     9061 tgtggtagat ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca
     9121 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
     9181 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
     9241 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
     9301 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     9361 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     9421 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     9481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     9541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9721 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     9841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9901 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9961 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    10021 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    10081 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    10141 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    10201 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    10261 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    10321 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    10381 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    10441 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    10501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    10561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
