LOCUS       sequence-466505-        8710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses UCP1 in mouse cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Choe M, Ekvik AE, Stalnaker G, Shin HR, Titov DV
  TITLE     Genetically encoded tool for manipulation of DeltaPsim identifies 
            its role as the driver of ISR induced by ATP synthase dysfunction.
  JOURNAL   Cell Chem Biol. 2025 Apr 17;32(4):620-630.e6. doi: 
            10.1016/j.chembiol.2025.03.007.
  PUBMED    40250406
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2025.03"; journalName: "Cell Chem Biol"; date: 
            "2025-04-17- 17"; volume: "32"; issue: "4"; pages: "620-630.e6"
COMMENT     Sequence Label: pLVX-TRE3G-UCP1
FEATURES             Location/Qualifiers
     source          1..8710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2564..3163)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2647..2667)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3144..3163
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3184..3683)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3232..3251)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3625..3648
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     regulatory      4655..4664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4671..5020)
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     primer_bind     complement(4708..4732)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4778..4796
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4814..4832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4850..4868
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4886..4904
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4922..4940
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4958..4976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4994..5012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    5083..5198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(5461..5505)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5690..5923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6420..6545
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6592..7225
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7393..7527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7417..7436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7471..7490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        7575..7679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7680..7748,7749..8540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7898..7917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgc ccgtcaggca ccgggcttgc
     2581 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     2641 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     2701 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     2761 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     2821 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     2881 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     2941 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     3001 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt
     3061 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     3121 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     3181 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     3241 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     3301 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     3361 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     3421 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     3481 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     3541 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
     3601 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     3661 tgggaaaagc gcctccccta cccggtagaa ttccatatga cgcgtcccgg ggcggccgcg
     3721 ggcccggatc cttatgtggt acaatccact gtctgtctgg acttcatcag ctcttttttc
     3781 agctgttcaa agcacacaaa catgatgacg ttccaggacc cgagtcgcag aaaagaagcc
     3841 acaaaccctt tgaaaaaggc cgtcggtcct tccttggtgt acatggacat cgcacagctt
     3901 ggtacgcttg ggtactgtcc tggcagagag ttgatgaatc ttgtttttac cacatccact
     3961 ggagaggcca ggagtgtggt gcaaaacccg gcaacaagag ctgacagtaa atggcagggg
     4021 acgtcatctg ccagtatttt gttgtttaca agggccccct tcatgaggtc atatgttacc
     4081 agctctgtac aattgatgat gacatttctc attagattag gggtcgtccc tttccaaagt
     4141 gttgacaagc tttctgtggt ggctataact ctgtaagcat tgtaggtccc cgtgtagcgg
     4201 ggtttgatcc catgcagatg gctctgggct tgcattctga ccttcacgac ctctgtaggc
     4261 tgcccaatga acactgccac acctccagtc attaagccgg ctgagatctt gtttccgaga
     4321 gaggcaggtg tttctctccc tgaagagaag tactcttgga ctgagtcgta gaggccaatc
     4381 ctgagtgagg caaagctgat ttgcctctga atgcccgcag gcagaccgct gtacagtttc
     4441 ggcaatcctt ctgtttttgc cagggtggtg atggtcccta ggacaccttt atacctaatg
     4501 gtactggaag cctggccttc accttggatc tgaaggcgga ctttggcagt gtccagcggg
     4561 aaggtgatga tatctgccag gcaagctgaa actccggctg agaagatctt gacccccatg
     4621 gtgggttgca cttcggaagt tgtcgggttc acccccatgg tggcggatcc aagtataaga
     4681 caaaagtgtt gtggaattgc tccaggcgat ctgacggttc actaaacgag ctctgctttt
     4741 ataggcgccc accgtacacg cctacctcga catacgttct ctatcactga tagggagtaa
     4801 actggatata cgttctctat cactgatagg gagtaaactg tagatacgtt ctctatcact
     4861 gatagggagt aaactggtca tacgttctct atcactgata gggagtaaac tccttatacg
     4921 ttctctatca ctgataggga gtaaagtctg catacgttct ctatcactga tagggagtaa
     4981 actcttcata cgttctctat cactgatagg gagtaaactc gagtgaagac gaaagggcct
     5041 catcgataaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta
     5101 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     5161 cctgcactgt accccccaat cccccctttt cttttaaagg cgataccgtc gagatccgtt
     5221 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
     5281 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     5341 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     5401 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     5461 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     5521 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     5581 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     5641 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     5701 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     5761 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     5821 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     5881 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     5941 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     6001 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
     6061 tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc
     6121 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag
     6181 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
     6241 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact
     6301 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
     6361 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
     6421 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
     6481 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
     6541 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
     6601 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     6661 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     6721 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg
     6781 caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg
     6841 ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag
     6901 ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca
     6961 aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt
     7021 aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact
     7081 agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc
     7141 tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt
     7201 ctttgggagt gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca
     7261 atttctttaa aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag
     7321 gtaattggca gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg
     7381 agttatccag ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     7441 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     7501 accattataa gctgcaataa acaagttgat ctcccgatcc gtcgacgtca ggtggcactt
     7561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7921 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8701 aaggatcttc
//
