LOCUS       sequence-466509-       12011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant version of KDM6A with increased oxygen affinity in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Chakraborty AA, Laukka T, Myllykoski M, Ringel AE, Booker MA, 
            Tolstorukov MY, Meng YJ, Meier SR, Jennings RB, Creech AL, Herbert 
            ZT, McBrayer SK, Olenchock BA, Jaffe JD, Haigis MC, Beroukhim R, 
            Signoretti S, Koivunen P, Kaelin WG Jr
  TITLE     Histone demethylase KDM6A directly senses oxygen to control 
            chromatin and cell fate.
  JOURNAL   Science. 2019 Mar 15;363(6432):1217-1222. doi: 
            10.1126/science.aaw1026. Epub 2019 Mar 14.
  PUBMED    30872525
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaw1026"; journalName: "Science"; date: 
            "2019-03-15- 15"; volume: "363"; issue: "6432"; pages: "1217-1222"
COMMENT     Sequence Label: pLX304-KDM6A-M1190T/E1335D
FEATURES             Location/Qualifiers
     source          1..12011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      646..655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             658..684
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4910..4934)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4936..4977
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5019..5607
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5072..5092)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5490..5501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5679..5912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5990..6111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6027..6046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6081..6100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6151..6286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6306..6325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6307..6325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6335..6352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6335..6351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6344..6366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6493..6948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6580..6599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6790..6811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6974..7078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7079..7147,7148..7939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7297..7316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8110..8698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8599..8618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8852..8869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8986..9007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9022..9027,9028..9045,9046..9052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9060..9076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9065..9087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9084..9100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9084..9100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9119..9139
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9121..9139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9167..9393
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9394..9574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9621..9746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10239..10472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10657..10701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10876..11376
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     11295..11313
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             11388..11783
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    11843..11960
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagcaggct ctttaaagga accaattcag tcgacgccac catggcatac
      661 ccatacgatg ttccagatta cgctatgaaa tcctgcggag tgtcgctcgc taccgccgcc
      721 gctgccgccg ccgctttcgg tgatgaggaa aagaaaatgg cggcgggaaa agcgagcggc
      781 gagagcgagg aggcgtcccc cagcctgaca gccgaggaga gggaggcgct cggcggactg
      841 gacagccgcc tctttgggtt cgtgagattt catgaagatg gcgccaggac gaaggcccta
      901 ctgggcaagg ctgttcgctg ctatgaatct ctaatcttaa aagctgaagg aaaagtggag
      961 tctgatttct tttgtcaatt aggtcacttc aacctcttat tggaagatta tccaaaagca
     1021 ttatctgcat accagaggta ctacagttta caatctgact actggaagaa tgctgccttt
     1081 ttatatggtc ttggtttggt ctacttccat tataatgcat ttcaatgggc aattaaagca
     1141 tttcaggagg tgctttatgt tgatcccagc ttttgtcgag ccaaggaaat tcatttacga
     1201 cttgggctta tgttcaaagt gaacacagac tatgagtcta gtttaaagca ttttcagtta
     1261 gctttggttg actgtaatcc ctgcactttg tccaatgctg aaattcaatt tcacattgca
     1321 cacttatatg aaacccagag gaaatatcat tctgcaaaag aagcttatga acaacttttg
     1381 cagacagaga atctttctgc acaagtaaaa gcaactgtct tacaacagtt aggttggatg
     1441 catcacactg tagatctcct gggagataaa gccaccaagg aaagctatgc tattcagtat
     1501 ctccaaaagt ccttggaggc agatcctaat tctggccagt cctggtattt cctcggaagg
     1561 tgctattcaa gtattgggaa agttcaggat gcctttatat cttacaggca gtctattgat
     1621 aaatcagaag caagtgcaga tacatggtgt tcaataggtg tgctatatca gcagcaaaat
     1681 cagcccatgg atgctttaca ggcctatatt tgtgctgtac aattggacca tggccatgct
     1741 gcagcctgga tggacctagg cactctctac gaatcctgca accagcctca ggatgccatt
     1801 aaatgctact taaatgcaac tagaagcaaa agttgtagta atacctctgc acttgcagca
     1861 cgaattcagt atttacaggc atgtaaacct catcatccaa atactgaacc tgtattaggc
     1921 ctcagtcaaa caccaatttc acaacaatcc ttgccactac acatgattcc ttctagccaa
     1981 gtagatgacc tgtccagtcc tgccaagagg aaaagaacat ctagtccgac aaagaatact
     2041 tctgacaatt ggagtggtgg acatgctgtg tcacatcctc cagtacagca acaagctcat
     2101 tcatggtgtt tgacaccaca gaaattacag catttggaac agctccgcgc aaatagaaat
     2161 aatttaaatc cagcacagaa actgatgctg gaacagctgg aaagtcagtt tgtcttaatg
     2221 caacaacacc aaatgagacc aacaggagtt gcacaggtac gatctactgg aattcctaat
     2281 gggccaacag ctgactcatc actgcctaca aactcagtct ctggccagca gccacagctt
     2341 gctctgacca gagtgcctag cgtctctcag cctggagtcc gtcctgcctg ccctgggcag
     2401 cctttggcca atggaccctt ttctgcaggc catgttccct gtagcacatc aagaacgctg
     2461 ggaagtacag acactatttt gataggcaat aatcatataa caggaagtgg aagtaatgga
     2521 aacgtgcctt acctgcagcg aaacgcactc actctacctc ataaccgcac aaacctgacc
     2581 agcagcgcag aggagccgtg gaaaaaccaa ctatctaact ccactcaggg gcttcacaaa
     2641 ggtcagagtt cacattcggc aggtcctaat ggtgaacgac ctctctcttc cactgggcct
     2701 tcccagcatc tccaggcagc tggctctggt attcagaatc agaacggaca tcccaccctg
     2761 cctagcaatt cagtaacaca gggggctgct ctcaatcacc tctcctctca cactgctacc
     2821 tcaggtggac aacaaggcat taccttaacc aaagagagca agccttcagg aaacatattg
     2881 acggtgcctg aaacaagcag gcacactgga gagacaccta acagcactgc cagtgtcgag
     2941 ggacttccta atcatgtcca tcagatgacg gcagatgctg tttgcagtcc tagccatgga
     3001 gattctaagt caccaggttt actaagttca gacaatcctc agctctctgc cttgttgatg
     3061 ggaaaagcca ataacaatgt gggtactgga acctgtgaca aagtcaataa catccaccca
     3121 gctgttcata caaagactga taactctgtt gcctcttcac catcttcagc catttcaaca
     3181 gcaacacctt ctccaaaatc cactgagcag acaaccacaa acagtgttac cagccttaac
     3241 agccctcaca gtgggctaca cacaattaat ggagaaggga tggaagaatc tcagagcccc
     3301 atgaaaacag atctgcttct ggttaaccac aaacctagtc cacagatcat accatcaatg
     3361 tctgtgtcca tataccccag ctcagcagaa gttctgaagg catgcaggaa tctaggtaaa
     3421 aatggcttat ctaacagtag cattttgttg gataaatgtc caccaccaag accaccatct
     3481 tcaccatacc ctcccttgcc aaaggacaag ttgaatccac ctacacctag tatttacttg
     3541 gaaaataaac gtgatgcttt ctttcctcca ttacatcaat tttgtacaaa tccgaacaac
     3601 cctgttacag taatacgtgg ccttgctgga gctcttaagt tagacctggg acttttctct
     3661 actaaaactt tggtggaagc taacaatgaa catatggtag aagtgaggac acagttgttg
     3721 cagccagcag atgaaaactg ggatcccact ggaacaaaga aaatctggca ttgtgaaagt
     3781 aatagatctc atactacaat tgctaaatat gcacagtacc aggcctcctc attccaggaa
     3841 tcattgagag aagaaaatga aaaaagaagt catcataaag accactcaga tagtgaatct
     3901 acatcgtcag ataattctgg gaggaggagg aaaggaccct ttaaaaccat aaagtttggg
     3961 accaatattg acctatctga tgacaaaaag tggaagttgc agctacatga gctgactaaa
     4021 cttcctgctt ttgtgcgtgt cgtatcagca ggaaatcttc taagccatgt tggtcatacc
     4081 atattgggca tgaacacagt tcaactatac atgaaagttc cagggagcag aacaccaggt
     4141 catcaggaaa ataacaactt ctgttcagtt aacataaata ttggcccagg tgactgtgaa
     4201 tggtttgttg ttcctgaagg ttactggggt gttctgaatg acttctgtga aaaaaataat
     4261 ttgaatttcc taacgggttc ttggtggccc aatcttgaag atctttatga agcaaatgtt
     4321 ccagtgtata ggtttattca gcgacctgga gatttggtct ggataaatgc aggcactgtt
     4381 cattgggttc aggctattgg ctggtgcaac aacattgctt ggaatgttgg tccacttaca
     4441 gcctgccagt ataaattggc agtggaacgg tacgaatgga acaaattgca aagtgtgaag
     4501 tcaatagtac ccatggttca tctttcctgg aatatggcac gaaatatcaa ggtctcagat
     4561 ccaaagcttt ttgaaatgat taagtattgt cttctaagaa ctctgaagca atgtcagaca
     4621 ttgagggaag ctctcattgc tgcaggaaaa gagattatat ggcatgggcg gacaaaagaa
     4681 gaaccagctc attactgtag catttgtgat gtggaggttt ttgatctgct ttttgtcact
     4741 aatgagagta attcacgaaa gacctacata gtacattgcc aagattgtgc acgaaaaaca
     4801 agcggaaact tggaaaactt tgtggtgcta gaacagtaca aaatggagga cctgatgcaa
     4861 gtctatgacc aatttacatt agctcctcca ttaccatccg cctcatcttg cccaactttc
     4921 ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     4981 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa
     5041 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5101 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5161 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5221 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5281 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5341 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5401 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5461 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5521 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5581 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     5641 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5701 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5761 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5821 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5881 acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat
     5941 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6121 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6181 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6241 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     6301 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6481 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     8281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8941 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     9001 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     9061 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     9121 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     9181 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     9241 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     9301 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     9361 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     9421 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     9481 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     9541 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     9601 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     9661 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     9721 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9781 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9841 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     9901 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9961 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    10021 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    10081 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    10141 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    10201 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    10261 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    10321 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    10381 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    10441 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    10501 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    10561 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    10621 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    10681 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    10741 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10801 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10861 accccgaggg gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
    10921 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
    10981 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
    11041 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
    11101 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
    11161 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
    11221 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
    11281 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
    11341 tccctcgttg accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt
    11401 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
    11461 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
    11521 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
    11581 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
    11641 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
    11701 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
    11761 cctctggtta tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac
    11821 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    11881 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    11941 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    12001 ggctcgaggg g
//
