LOCUS       sequence-466526-       15145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suppressor screen mutant in full-length Prp8 on a TRP plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15145)
  TITLE     Hoskins lab plasmids 2025
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRS424 Prp8 V1862L
FEATURES             Location/Qualifiers
     source          1..15145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1518..1535)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2334..2353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2335..2353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2363..2380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2363..2379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2372..2394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2520..2975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2607..2626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2817..2838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3078..3752)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3753..4034)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(4055..4074)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4174..4196
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4234..4252)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      4293..5635
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    4625..4672
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5662..5766
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5767..5835,5836..6627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5985..6004)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6798..7386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7287..7306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7540..7557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7674..7695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7710..7715,7716..7733,7734..7740)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7748..7764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7753..7775
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7772..7788
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7772..7788
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7807..7827
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7809..7827
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tagcacgtag ttcttcaaga gcttttggta agagacctta actttaacgg gatactcctc
       61 gtcaggatgt tgcaaaaacc attttttagc cagggcaaca tcttgtgctc tcaccatttt
      121 tcctttagtt ctattaaatg gaaaaggtga atgatataac gacatcgcat cttttgtatt
      181 tggcaaaata agttcttctt cttcagccaa aagaggtgca aaatcttcag gtaaagtgaa
      241 atcaccgttt tcctttgtat ttgatacgtt gagggatgaa ttattatcaa taaagtgagg
      301 tattgggttc agcgatggat caaaaaaaag tgcaggcgtg tcgtactcct catcgttctg
      361 gataatacac gacactggat tattatacca tggtatgcgc actgaacggg gccgggaatt
      421 atatagatga ggaaacgcta ccttatattc gcttctaata ggaaccctaa atattactct
      481 atctatagag ttgaattcgt tataatcttc ctcctcttct cttgggtata aaggttcaaa
      541 tttaggacct ccagggattg cgttattaag ggctttaccg ttaaaaaaag attttttatc
      601 gaaaagataa tagtaatttt tgtcagttac ttcatctcta agtggcgttg ataacctata
      661 taagttcgac atttcaggta aatcaaaact ccacttttta taagatgtac cattgacctt
      721 tttactatcc tcctccaagg gccgggaatc gtagagccaa tctttaacat attcatcatc
      781 ttgagagtct aagggaagat ttataggatc cagtggctcg atattttcta tgtgctgttc
      841 atatgacaga ggtggttcgt catcatcgaa aggtggaaat ctcattcttt taaaatgtgt
      901 tctatctcgt ttttctctcc tcattgctat ccaagtagct gaccattggg cagtatatac
      961 aggctctatt actcttggtg tttcattcac aaatgttatg gctcctgaag tgtgatataa
     1021 aactttaact tctttggctt gctcccatgg atgaggcatg ttctctaaca attttaaaat
     1081 cgcgtgcggt aaatacttta gagcgcctag aaaagccttt ttatcagtat tgtacatttt
     1141 ggaggccatg tcactgtgag tattaataat ctttcgcaga tgttccggtg gcatctcggc
     1201 tttaggagta tataaattgc ttctctttgc ctttttggtc attttttttg cacgctttct
     1261 tgattttcga gtgacgaaag tatcttttcc tatgtccaat tttctctttc catgtaattc
     1321 tactttcttt tctgctttag tctctaattc atctagacct ggagggggtg gtggcggtgg
     1381 taatgtgaag tccacaattt cttcagcgtt tatttcgaag ttgcttggag gaggtggtgg
     1441 aggcggaagg aatgtatcct catttaccga tgatggcacc atcggattat ccagttcttc
     1501 gatttcgtat ccaggcggtg gtggtggtgg tggtggaagt gctaagtcgc tgtcctcttc
     1561 aaaaccagga ggtgggggcg gtagtccact catctttctt tgacgctatt ttttttttgg
     1621 aaggaagttc aatcgcacaa gtattttcta tggtcttcgt ttctcaaatt gtattcctaa
     1681 ctttgttgct tttgtactag ccgtattacc tcggttcatc cttctgccat ggtcacttta
     1741 aatagctata tagcctggca tttataaaaa tttcatatca tttagtacga atagctatgt
     1801 gtatgttcac ttcactacat atgccttctg cactcccgag ccagcatacg agcctcttca
     1861 atctcttgag acgttccact aggtaccccc atggcataat cataagccaa ttggtagttt
     1921 cctgccttaa tctcaaatat tgccagccaa agcctagcct tcacggtttc atctgttact
     1981 ataccttcca gaagttcttc cacatctaca catttcatca tgaacttctt acattcttgc
     2041 aagtcttcaa gttcttcata tagttgcgct aaccggtaat atattgaagt attttgatcg
     2101 accgtttgtg aagcttttat ggatcttttg tagcatttta tagcttctac cttatttccc
     2161 gtcttactat aacattctcc caatacttgc caaatccgac gatcccaagg tttcaaagtg
     2221 caagctttct ggaagtagta aagagaatat aaatgcatgt ccaggagagc ataagcctgg
     2281 cccaaaccaa accatgcttt gaagtctcga gggggggccc ggtacccaat tcgccctata
     2341 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2401 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2461 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     2521 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     2581 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     2641 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     2701 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     2761 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2821 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2881 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2941 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     3001 catctgtgcg gtatttcaca ccgcatagat cggcaagtgc acaaacaata cttaaataaa
     3061 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
     3121 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
     3181 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt
     3241 tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct
     3301 gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca
     3361 ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg
     3421 aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatatc aatgccgtaa
     3481 tcattgacca gagccaaaac atcctcctta ggttgattac gaaacacgcc aaccaagtat
     3541 ttcggagtgc ctgaactatt tttatatgct tttacaagac ttgaaatttt ccttgcaata
     3601 accgggtcaa ttgttctctt tctattgggc acacatataa tacccagcaa gtcagcatcg
     3661 gaatctagag cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga
     3721 ccagaactac ctgtgaaatt aataacagac atactccaag ctgcctttgt gtgcttaatc
     3781 acgtatactc acgtgctcaa tagtcaccaa tgccctccct cttggccctc tccttttctt
     3841 ttttcgaccg aattaattct taatcggcaa aaaaagaaaa gctccggatc aagattgtac
     3901 gtaaggtgac aagctatttt tcaataaaga atatcttcca ctactgccat ctggcgtcat
     3961 aactgcaaag tacacatata ttacgatgct gttctattaa atgcttccta tattatatat
     4021 atagtaatgt cgttgatcta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4081 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4141 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4201 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4261 ttaatgtcat gataataatg gtttcttagt atgatccaat atcaaaggaa atgatagcat
     4321 tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta aagggtagtg
     4381 ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag gtactagact
     4441 acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa acacgcacta
     4501 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa
     4561 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg
     4621 ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct
     4681 tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg
     4741 taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
     4801 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
     4861 aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat tactctttag
     4921 acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa
     4981 acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac
     5041 caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg
     5101 tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta
     5161 atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa
     5221 gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta
     5281 tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct
     5341 cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct
     5401 ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     5461 gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata
     5521 gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     5581 ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttcaggt
     5641 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5701 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5761 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5821 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5881 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5941 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6001 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6061 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6121 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6181 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6241 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6301 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6361 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6421 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6481 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6541 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6601 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6661 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6721 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6781 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6841 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6901 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6961 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7021 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7081 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7141 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7201 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7261 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7321 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7381 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7441 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7501 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7561 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7621 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7681 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7741 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7801 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg cggtggcggc
     7861 cgctctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc
     7921 cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta
     7981 cgcgatcatg gcgaccacac ccgtcctgtg gatcttcaat ttctcctctc gattttgtgt
     8041 tatcattctt cccacaactt tcctgggctc cacttttttg attggtgttg ccttgaattg
     8101 ttatctgact ctcactcttg cgtttctcaa catacttttg agttaaaata tctatcaaag
     8161 agtcatcgcc atcgttgcta tctcctgttg aatccatttt atctacttct tctgcattca
     8221 cctctgcttt tttttcaacg ttgggtgcct tgatgggtga agatggcaaa tcgctgaatt
     8281 tcgttatcgt cggttttata gttaaatcgc cctctatatc atcgagaata tcatctatat
     8341 tacggagaga ctccctggta gatatttctg ctggtttagg acgcacttgc tcatttccat
     8401 tttcttgaat gagttgattt acgtcagcgt tgggtacttg gatatttgtg aaactaagaa
     8461 cagttttcag tcgattaggg actataggtg tagaagaggg attttggaca tcttcgtagt
     8521 catcagagta ttcggcagcg cttgttgttt tgtcggacat atctcgttgt aaaggtgaat
     8581 atttccatat tacttcttcc atggggccgt cctcgtcttg agtgaaatcg tgatgcctac
     8641 tcaaattaga catttcccgt ttcggtttaa cttgtgaatt tggtaattgc tttacgctct
     8701 tgctaactgc tttgtttata tcttttgttc ttgatgtatt tcgtacttgt gaaacggcta
     8761 ttgactttag gacacttgca ttggatacct tggtgttatt cccatttaag ttatttatag
     8821 gagcaaaagc atactttttt ttcctcttag tttgcttaga taatatcgcc tttgaatcat
     8881 tttgtattat ttctttttcc tctgtcttct tcgcaggtga aacagatata ctcgcagacc
     8941 tcttgttctt ctgtggcgtt ccgggcatcc tgacgatctc ttcaattgtc agtgtttgct
     9001 tgcacaaaat gagtactcac ttgagtatgt tttctcccaa ttttgttttg aggctgccgt
     9061 aatgattgta actttatctt caaaaatgta acacaagttt tgccataaat agcgtaagat
     9121 cagttaaata tatataagtt ctttctgccg cacggaattg ctttattgcg cgaaatgaaa
     9181 acaagaatat ccaatttcta tatgaaggac ataatggaat gggagtgaca acggaccatt
     9241 ttctgtccga acgatgtcta tctcctttgg ttactttttg aaatagaatc acagtcaatg
     9301 accaattttc acatacttat agcttttcat agtgcggaaa gcgtagcttt gaagtagttc
     9361 cgaaattata atacatcatc gtgtggtagg tagcaactac gacgggcata aaaaatgcgt
     9421 tgaatattat acatacaaac ttatacttac atatatatct atgaaataac agattccagt
     9481 ttattgggga atatattcag ctaaatacat cgatttgttc ggcctctaac tcttcgtccc
     9541 ccgccaattc gctaaactgt aaaaagtgta ctggacgatg catctcatta tagaattcca
     9601 aaggaatgcc gtatttaaaa ttgtagtcac cttcttggtt aaatgctgtt cccataaatg
     9661 tgtagttcca aacgtttcca gatggaatta taaaattacc tgtgatacgg tcagagagaa
     9721 gtaattgggc atgcgtgctg aaagtaggct caaacccttc cgatagcacg ttcattatat
     9781 ccttattttc ttcaccccat tggtaacctt cgtcggtcag gttataagcg cttaatgata
     9841 ctgatcccgg ggtggaaaaa attgaaatat caatgcagtc tcttttctta tccgcaaaaa
     9901 gtttagaatg agttgctact tcagacgcag ccatgaattt aagttcttct gtctgggtat
     9961 ggatccaacc gagtagttca aggccttcag tatccggaag gtctccgatg tcagggatgt
    10021 tgcttatctg aacggatcct acgtggccta attgaggtac cagcacaaca gtcttgattt
    10081 ctttcacctt cggatgatct tttgctgaca taccataaat aaaggccgcc acctggattt
    10141 tcacatcgga tatctcaata aacttcttta ataaattttt tggaaggacg taaacattct
    10201 gttcctccac gaaatcatca gccgaaacat aaatgttttt taacctcaaa tataatagtg
    10261 tattagcaat tgcagatttt ctccattcat tctttgaact aaacgtttga ctttcgtaat
    10321 cagctgaagc tactacaact atctcctcac cttgcgcatt gatagtttta gttttcataa
    10381 cagttgatgc acctgcagct tcttcgtcgt tctgcttttc tgacctggca gcttctaatt
    10441 cagccatttt ttgtctcttc acagatggtg ccttgatatt ttgacccaat ataatatctt
    10501 taatttccgt ttgagttaag gcagatatat tgacattgta ttttctgccg tactcagtca
    10561 aaatcaaatc tctcatttga gattcgatag tgatccattg ttcatcagtg aatgaaggcc
    10621 ataaatggta cgatttgata gtgatcgtgg ggtcactgag tagaatcatt ttggcacttt
    10681 cctcgttcgt ctttaaagct cttaaaagta gtgtaagtct agaaaatgca gtgtacgatg
    10741 aaatacggtc taaccaatca tcataaatgt taaacagaac catctgaggt tcagtagctt
    10801 tcatcacaac atcagaaagt ttatctattg acattgcagc tgaaaatggt agtcgcagct
    10861 ctgttggcct gattgctatg ttaggaaagt caagcatgtg aacctctaaa ggatcaagca
    10921 ttgcctttct tgtgacgata atttgttttg gctgctcttc tttgggcaaa gatctcacaa
    10981 gagcactaac ttcctcagct gttttccatt tggccaattg gcttaaacgt ttttgaccgg
    11041 cccatacaga ggtatggata attttcagaa acagatgccc agtctttgga tttaaagtga
    11101 aaatgcaacc attaattgct tttgtagcaa cgttcccttc aaaagtcttg tggagagtaa
    11161 ctctatacac attagtgtcg tcaacgaaga gtttaatgtc gttattgaat aactcggcat
    11221 agtttgagga gtttaaaaat ggttcctgta cactagattg atatatttga agaccttttc
    11281 taatacgttc acgaaggaca tataatgctg ggttagcttt cataattgtt ctcatactat
    11341 tttgtatcaa aggttttaat ccgttgaacc aattaccata ggcatcatac atgttataag
    11401 ccaaatcgat accgatcata acacctgttg gggagggata catgctgaca ttatctgttg
    11461 tataatcgag gaattttgcc ctcacataac gagatatatc atgagaatca taatcaccgt
    11521 atctcaattg tacatcaaac cacattttgt tggtaatcaa tcccttaaat gagtcatttg
    11581 tttcatgtaa tagggagggt tttgaaactt cccattcatg aacactttcc atagttatgt
    11641 cagcggcaga tgaattcatc ttgtacgatt tgcggggatg cactgtttct ttagtcacag
    11701 attcaatttg caaaacatct agctcaccat cgagaatttg acaaatatca aaaactatac
    11761 tttcatgtat tttttgccac aaatgagcac ggaatatttg aattaaagat atttttaacg
    11821 taggaatttt accatggagg aagataccag tcagatccaa ttgcaccaag aaaccgacat
    11881 agacatttgc tctgttaata gttggtgacc accataaggt aaatctacga tttggaattt
    11941 ggcttaaacc agttctttga gcatgagtta gcttcttaaa ttgcatggaa tcctcaaacc
    12001 cggacgcctt ttcccaaaac aaaccttccc atgaattaaa accagtcccc ttaaataaag
    12061 tgtgttccaa aattgtttca atacctccta gggcttgaat cacatcagtt ctataggcat
    12121 ttaaattcca taatttaccg tcatggtggg agtttgtcca ccaaaagggg ctgtttctct
    12181 ctagagagta ctgtttaaat tccctacgga ttctgtgacc tctgtcatac gctagagtat
    12241 gtcgatctct ttgaaataaa gtgctaatac gaggtatacc acgatcccac gatccttcaa
    12301 gttcttcaaa agctaacctc ctattctgct gaatagcttc ctgccttttc gttgcatact
    12361 cggcccacac cctttgagag tctaaaaatt cattttccca agtagtgatg taacggaaga
    12421 tagttgggat taatttttca tcctcgtgcg tcataccagc acgaaaatgc gtaattccag
    12481 tatcggtctg ttttgaccaa cttaagtcag atgcaggaat taaaatatga gaagcactaa
    12541 tcatacccaa cccaccaagt tcttttggtg tataaaaaac agcaggtgga aatcttgttg
    12601 gcattttcga atttaaacca agtttgacac ggttttgaat tcttgtttct cctttcacca
    12661 ggatatctaa taacggctcc gtggcaacaa tggcttctct aaagtatgtg aaaagggata
    12721 tcagtgaagt gttccattta gcggccactt tagtaaaagt ggttgaaccg gaggccatca
    12781 atatgcctct tattctgctg tcaaattttt tgatttcttc ttcagatacc tttaaatatg
    12841 cttttgcagt tctttgcttg gttctttcgt caactaaatc ccaaacacct tcatcgttag
    12901 aaaccacctc ttccattctt tgtcttggta atattctgac ttcaaaacca cacatagaga
    12961 agagtaagtt cggattgttt ttactgtata cggaaacaaa agcattttcc catttaattg
    13021 acgttaacga agttggcacc ctactttgaa tttcccaaaa cactgctcgc cctaggttaa
    13081 cgtcctgacg tattagcctc atgcgcgaat ctttgggcca gcattttcta ttattgtatc
    13141 cgatgctgtt ttcaaagttt ggatctggat tctctgccaa gtattcatca gtcaattcct
    13201 ctccctcatc ttcttcaaag tgaaaaagca tatatatacg atctaaatat ctggtgtaaa
    13261 gtctgatcgg atgtgccttt tctacttctt tgctcttgaa ttgcataaat tcatttggat
    13321 tgttagctgg accagccaaa tctgtagccc tttcctgacc caatagtaaa agatctataa
    13381 ctagtccgta atattggaat atgaaagaag cgaacttcaa cccgcgtatt aagccatatt
    13441 tgttgacgtg actcatatct ttaaagttga taacaacatt attttttgca gtgatatagt
    13501 cagcaatatt aggatctaca atcaggcgaa gtaatctatt aagtaaagta aagtcaattt
    13561 tttcggccat ttcacccaaa gtagtctcaa gcaaaactgc agattggcct ctggatacat
    13621 cccaaatttc agataggttg tttataccct gagtccactt gtataccaga agaggtggta
    13681 tctctgaatc acttggttta atccagtttg ggaaaagctt tcgttggtca gcttcgtacc
    13741 ataaatactg atcaagatat gcatcggtaa tcttttccaa gggatcaact gaataaacag
    13801 gggaaatgtt ttgatagttt tccatcatgg ttatatcaac aggcttaaaa actcgctggg
    13861 tcaacaagta ctttttgatt ctgttcaaag tatcgtgagg attatcataa gcctcctcta
    13921 tcaacgcaag ttcttccctt tcagatgcat ttaaacgtac ttttgatgcg tagacatctt
    13981 ttaggtcttc gagggcaaga actaatatct tagtatcatt tttgtaagtt aaaggtggaa
    14041 atggtattgg tgaaaagctc cttgattcaa gccactcaac cataacagaa aatatggtcg
    14101 tagcctcttc gggagtaatt tcgggaccgt ttttctgtat ttgcctttga cgttcctgct
    14161 catttttgat ccaaagtctc gttaaacgcc ccagattttt tttaaccata gtcttttcga
    14221 cgtgagctcc acgctttata cgttctcgat tgtaatgagc tgcagatacc caggcatcag
    14281 ctttggattt tatataccgt tcaattattt ttttgattgg ggctggcata ccaggaacat
    14341 cccatggaat attagccttc caacatctcc aagcctcact taaatgttgc agtatagttc
    14401 ttgccttttt ctgtcgaata ctctcaggca tcatttctaa aatgtcatcc atgaccgagt
    14461 ttcgtaactc aagatcgtag taggcgtcaa gtctttgttt agttgtagtt ttgacaattt
    14521 cattagaacg gccttcaaat tgacgtgtga taaggtttcc aatatatctt tcgagtagtg
    14581 gtatagtgcc tcttaagaaa tttagccaca ctctccacgc aggttgccaa aatccacaac
    14641 caggtccttt tcccaaattt ttattaaact tataatatat gatatgtttc agatctttac
    14701 atgcgcggat ttgatgcatt actttatatt tataacgata aataccggtc aattgtccta
    14761 tgtggttcaa aatataatgt ataccatctg ctaactggaa ggcatctaca tttcctaacc
    14821 taaactgcac atgtgtatct acaatcagtt tcatcatttt tagcaattca cgcatcaaat
    14881 gaaatgagtt tccaagtctt gactttttac gttcttttgt ggttaaggtt ttagttggtt
    14941 ttaaattgaa attgtagtcg agatgtaaat aagttaggcc ctttctgtgg attaataaat
    15001 ttaacatatt gtgtccttgt cgacatagct ggagtcctgc ctctacccaa tcaattgtag
    15061 tttgttgaaa atacttggta ttttttaaac tcttcaataa cttagttttg ttgtggtttg
    15121 ttggcaaagt agggtgcaat tcatt
//
