LOCUS       sequence-466532-        8657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of WT SRP19 (siRNA resistant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Xi X, Liu L, Tuano N, Tailhades J, Mouradov D, Steen J, Sieber O, 
            Cryle M, Nguyen-Dumont T, Segelov E, Rosenbluh J
  TITLE     SRP19 and the protein secretion machinery is a targetable 
            vulnerability in cancers with APC loss.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Apr 15;122(15):e2409677122. doi: 
            10.1073/pnas.2409677122. Epub 2025 Apr 10.
  PUBMED    40208946
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-04-15"; volume: "122(15):e2409677122. 
            doi"; pages: " 10.1073/pnas.2409677122. Epub 2025 Apr 10"
COMMENT     Sequence Label: SRP19 (siRNA resistant) in pLX311
FEATURES             Location/Qualifiers
     source          1..8657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1752..1776)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1784..1825
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1876..2464
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1929..1949)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2347..2358)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2536..2769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2847..2968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2884..2903)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2938..2957
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3008..3143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3163..3182)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3164..3182)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3192..3209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3192..3208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3201..3223)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3350..3805
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3437..3456)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3647..3668
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3831..3935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3936..4004,4005..4796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4154..4173)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4967..5555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5456..5475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5709..5726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5843..5864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5879..5884,5885..5902,5903..5909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5917..5933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5922..5944
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5941..5957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5941..5957
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5976..5996
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5978..5996
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6024..6250
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6251..6431
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6478..6603
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7096..7329
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7514..7558
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7789..7999
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7811..8007
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7858..7993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8029..8424
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8498..8615
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agttggcatg
     1321 gcttgcgctg ccgcgcggtc cccggccgac caggacaggt ttatttgtat ctatcctgct
     1381 tatttaaata ataagaagac catcgcagag ggaaggcgaa tccccataag taaggctgtt
     1441 gaaaatccta cagctacaga gattcaagat gtatgttcag cagttggact taacgtattt
     1501 cttgagaaaa ataaaatgta ctctagagaa tggaatcgtg atgtccaata cagaggcaga
     1561 gtccgggtcc agctcaaaca ggaagatggg agcctctgcc ttgtacagtt cccatcacgt
     1621 aagtcagtaa tgttgtatgc agcagaaatg atacctaaac taaaaacaag gacacaaaaa
     1681 acaggaggtg ctgaccaaag tcttcaacag ggtgaaggca gcaagaaagg gaaaggaaag
     1741 aaaaagaaga cccccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa
     1801 ccctctcctc ggtctcgatt ctacgtagta atgaactagt accggttaag tcgacaatca
     1861 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1921 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1981 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2041 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2101 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2161 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2221 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2281 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2341 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2401 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2461 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     2521 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     2581 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2641 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2701 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2761 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     2821 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     2881 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2941 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3001 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3121 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     3181 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3241 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3301 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatcgga cgcgccctgt
     3361 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3421 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3481 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3541 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3601 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3661 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3721 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3781 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3841 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3901 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3961 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4021 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4081 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4141 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4201 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4261 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4321 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4381 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4441 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4501 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4561 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4621 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4681 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4741 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4801 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4861 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4921 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4981 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5041 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5101 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5161 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5221 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5281 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5341 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5401 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5461 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5521 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5581 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5641 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5701 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     5761 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     5821 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     5881 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     5941 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6001 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6061 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6121 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     6181 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     6241 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6301 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6361 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6421 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     6481 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     6541 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     6601 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     6661 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     6721 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     6781 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     6841 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     6901 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     6961 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7021 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7081 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7141 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     7201 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     7261 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     7321 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     7381 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     7441 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     7501 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     7561 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     7621 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     7681 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     7741 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7801 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7981 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccat ggccaagcct
     8041 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     8101 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     8161 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     8221 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     8281 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     8341 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     8401 ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aaggcgcgcc gttaacgaat
     8461 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     8521 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     8581 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     8641 atccactttg gcgccgg
//
