LOCUS       sequence-466537-       15145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suppressor screen mutant in full-length Prp8 on a TRP plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15145)
  TITLE     Hoskins lab plasmids 2025
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRS424 Prp8 S1584F
FEATURES             Location/Qualifiers
     source          1..15145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3834..3854)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3834..3852)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3873..3889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3873..3889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3886..3908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3897..3913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3921..3927,3928..3945,3946..3951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3966..3987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4104..4121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4275..4863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4355..4374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5034..5825,5826..5894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5657..5676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5895..5999)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6026..7368
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6989..7036)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7409..7427
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7465..7487)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7587..7606
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7627..7908
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7909..8583
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8686..9141)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8823..8844)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9035..9054
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9267..9289
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9281..9298
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9282..9298
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9308..9327
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9308..9326
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             10126..10143
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatgaattca tctgccgctg acataactat ggaaagtgtt catgaatggg aagtttcaaa
       61 accctcccta ttacatgaaa caaatgactc atttaaggga ttgattacca acaaaatgtg
      121 gtttgatgta caattgagat acggtgatta tgattctcat gatatatctc gttatgtgag
      181 ggcaaaattc ctcgattata caacagataa tgtcagcatg tatccctccc caacaggtgt
      241 tatgatcggt atcgatttgg cttataacat gtatgatgcc tatggtaatt ggttcaacgg
      301 attaaaacct ttgatacaaa atagtatgag aacaattatg aaagctaacc cagcattata
      361 tgtccttcgt gaacgtatta gaaaaggtct tcaaatatat caatctagtg tacaggaacc
      421 atttttaaac tcctcaaact atgccgagtt attcaataac gacattaaac tcttcgttga
      481 cgacactaat gtgtatagag ttactgtcca caagactttt gaagggaacg ttgctacaaa
      541 agcaattaat ggttgcattt tcactttaaa tccaaagact gggcatctgt ttctgaaaat
      601 tatccatacc tctgtatggg ccggtcaaaa acgtttaagc caattggcca aatggaaaac
      661 agctgaggaa gttagtgctc ttgtgagatc tttgcccaaa gaagagcagc caaaacaaat
      721 tatcgtcaca agaaaggcaa tgcttgatcc tttagaggtt cacatgcttg actttcctaa
      781 catagcaatc aggccaacag agctgcgact accattttca gctgcaatgt caatagataa
      841 actttctgat gttgtgatga aagctactga acctcagatg gttctgttta acatttatga
      901 tgattggtta gaccgtattt catcgtacac tgcattttct agacttacac tacttttaag
      961 agctttaaag acgaacgagg aaagtgccaa aatgattcta ctcagtgacc ccacgatcac
     1021 tatcaaatcg taccatttat ggccttcatt cactgatgaa caatggatca ctatcgaatc
     1081 tcaaatgaga gatttgattt tgactgagta cggcagaaaa tacaatgtca atatatctgc
     1141 cttaactcaa acggaaatta aagatattat attgggtcaa aatatcaagg caccatctgt
     1201 gaagagacaa aaaatggctg aattagaagc tgccaggtca gaaaagcaga acgacgaaga
     1261 agctgcaggt gcatcaactg ttatgaaaac taaaactatc aatgcgcaag gtgaggagat
     1321 agttgtagta gcttccgctg attacgaaag tcaaacgttt agttcaaaga atgaatggag
     1381 aaaatctgca attgctaata cactattata tttgaggtta aaaaacattt atgtttcggc
     1441 tgatgatttc gtggaggaac agaatgttta cgtccttcca aaaaatttat taaagaagtt
     1501 tattgagata tccgatgtga aaatccaggt ggcggccttt atttatggta tgtcagcaaa
     1561 agatcatccg aaggtgaaag aaatcaagac tgttgtgctg gtacctcaat taggccacgt
     1621 aggatccgtt cagataagca acatccctga catcggagac cttccggata ctgaaggcct
     1681 tgaactactc ggttggatcc atacccagac agaagaactt aaattcatgg ctgcgtctga
     1741 agtagcaact cattctaaac tttttgcgga taagaaaaga gactgcattg atatttcaat
     1801 tttttccacc ccgggatcag tatcattaag cgcttataac ctgaccgacg aaggttacca
     1861 atggggtgaa gaaaataagg atataatgaa cgtgctatcg gaagggtttg agcctacttt
     1921 cagcacgcat gcccaattac ttctctctga ccgtatcaca ggtaatttta taattccatc
     1981 tggaaacgtt tggaactaca catttatggg aacagcattt aaccaagaag gtgactacaa
     2041 ttttaaatac ggcattcctt tggaattcta taatgagatg catcgtccag tacacttttt
     2101 acagtttagc gaattggcgg gggacgaaga gttagaggcc gaacaaatcg atgtatttag
     2161 ctgaatatat tccccaataa actggaatct gttatttcat agatatatat gtaagtataa
     2221 gtttgtatgt ataatattca acgcattttt tatgcccgtc gtagttgcta cctaccacac
     2281 gatgatgtat tataatttcg gaactacttc aaagctacgc tttccgcact atgaaaagct
     2341 ataagtatgt gaaaattggt cattgactgt gattctattt caaaaagtaa ccaaaggaga
     2401 tagacatcgt tcggacagaa aatggtccgt tgtcactccc attccattat gtccttcata
     2461 tagaaattgg atattcttgt tttcatttcg cgcaataaag caattccgtg cggcagaaag
     2521 aacttatata tatttaactg atcttacgct atttatggca aaacttgtgt tacatttttg
     2581 aagataaagt tacaatcatt acggcagcct caaaacaaaa ttgggagaaa acatactcaa
     2641 gtgagtactc attttgtgca agcaaacact gacaattgaa gagatcgtca ggatgcccgg
     2701 aacgccacag aagaacaaga ggtctgcgag tatatctgtt tcacctgcga agaagacaga
     2761 ggaaaaagaa ataatacaaa atgattcaaa ggcgatatta tctaagcaaa ctaagaggaa
     2821 aaaaaagtat gcttttgctc ctataaataa cttaaatggg aagaacacca aggtatccaa
     2881 tgcaagtgtc ctaaagtcaa tagccgtttc acaagtacga aatacatcaa gaacaaaaga
     2941 tataaacaaa gcagttagca agagcgtaaa gcaattacca aattcacaag ttaaaccgaa
     3001 acgggaaatg tctaatttga gtaggcatca cgatttcact caagacgagg acggccccat
     3061 ggaagaagta atatggaaat attcaccttt acaacgagat atgtccgaca aaacaacaag
     3121 cgctgccgaa tactctgatg actacgaaga tgtccaaaat ccctcttcta cacctatagt
     3181 ccctaatcga ctgaaaactg ttcttagttt cacaaatatc caagtaccca acgctgacgt
     3241 aaatcaactc attcaagaaa atggaaatga gcaagtgcgt cctaaaccag cagaaatatc
     3301 taccagggag tctctccgta atatagatga tattctcgat gatatagagg gcgatttaac
     3361 tataaaaccg acgataacga aattcagcga tttgccatct tcacccatca aggcacccaa
     3421 cgttgaaaaa aaagcagagg tgaatgcaga agaagtagat aaaatggatt caacaggaga
     3481 tagcaacgat ggcgatgact ctttgataga tattttaact caaaagtatg ttgagaaacg
     3541 caagagtgag agtcagataa caattcaagg caacaccaat caaaaaagtg gagcccagga
     3601 aagttgtggg aagaatgata acacaaaatc gagaggagaa attgaagatc cacaggacgg
     3661 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
     3721 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
     3781 acgcatatag cgctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     3841 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4081 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6001 acatttcccc gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa
     6061 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     6121 gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa
     6181 caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca
     6241 gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg
     6301 ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt
     6361 tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt
     6421 taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc
     6481 cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc
     6541 atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata
     6601 gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat
     6661 atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt
     6721 tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag
     6781 gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat
     6841 agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc
     6901 aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca
     6961 gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact
     7021 tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc
     7081 tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata
     7141 tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct
     7201 atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg
     7261 gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat
     7321 tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac
     7381 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     7441 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7501 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7561 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7621 tagatcaacg acattactat atatataata taggaagcat ttaatagaac agcatcgtaa
     7681 tatatgtgta ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga
     7741 aaaatagctt gtcaccttac gtacaatctt gatccggagc ttttcttttt ttgccgatta
     7801 agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca ttggtgacta
     7861 ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat gtctgttatt
     7921 aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag cacagaggcc
     7981 gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt gcccaataga
     8041 aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa
     8101 aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat
     8161 gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg
     8221 tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa
     8281 gactgcaaca tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt
     8341 gattcagaag caggtgggac aggtgaactt ttggattgga actcgatttc tgactgggtt
     8401 ggaaggcaag agagccccga aagcttacat tttatgttag ctggtggact gacgccagaa
     8461 aatgttggtg atgcgcttag attaaatggc gttattggtg ttgatgtaag cggaggtgtg
     8521 gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa
     8581 taggttatta ctgagtagta tttatttaag tattgtttgt gcacttgccg atctatgcgg
     8641 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     8701 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     8761 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     8821 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     8881 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     8941 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     9001 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     9061 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     9121 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     9181 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     9241 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     9301 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgagacttc
     9361 aaagcatggt ttggtttggg ccaggcttat gctctcctgg acatgcattt atattctctt
     9421 tactacttcc agaaagcttg cactttgaaa ccttgggatc gtcggatttg gcaagtattg
     9481 ggagaatgtt atagtaagac gggaaataag gtagaagcta taaaatgcta caaaagatcc
     9541 ataaaagctt cacaaacggt cgatcaaaat acttcaatat attaccggtt agcgcaacta
     9601 tatgaagaac ttgaagactt gcaagaatgt aagaagttca tgatgaaatg tgtagatgtg
     9661 gaagaacttc tggaaggtat agtaacagat gaaaccgtga aggctaggct ttggctggca
     9721 atatttgaga ttaaggcagg aaactaccaa ttggcttatg attatgccat gggggtacct
     9781 agtggaacgt ctcaagagat tgaagaggct cgtatgctgg ctcgggagtg cagaaggcat
     9841 atgtagtgaa gtgaacatac acatagctat tcgtactaaa tgatatgaaa tttttataaa
     9901 tgccaggcta tatagctatt taaagtgacc atggcagaag gatgaaccga ggtaatacgg
     9961 ctagtacaaa agcaacaaag ttaggaatac aatttgagaa acgaagacca tagaaaatac
    10021 ttgtgcgatt gaacttcctt ccaaaaaaaa aatagcgtca aagaaagatg agtggactac
    10081 cgcccccacc tcctggtttt gaagaggaca gcgacttagc acttccacca ccaccaccac
    10141 caccgcctgg atacgaaatc gaagaactgg ataatccgat ggtgccatca tcggtaaatg
    10201 aggatacatt ccttccgcct ccaccacctc ctccaagcaa cttcgaaata aacgctgaag
    10261 aaattgtgga cttcacatta ccaccgccac caccccctcc aggtctagat gaattagaga
    10321 ctaaagcaga aaagaaagta gaattacatg gaaagagaaa attggacata ggaaaagata
    10381 ctttcgtcac tcgaaaatca agaaagcgtg caaaaaaaat gaccaaaaag gcaaagagaa
    10441 gcaatttata tactcctaaa gccgagatgc caccggaaca tctgcgaaag attattaata
    10501 ctcacagtga catggcctcc aaaatgtaca atactgataa aaaggctttt ctaggcgctc
    10561 taaagtattt accgcacgcg attttaaaat tgttagagaa catgcctcat ccatgggagc
    10621 aagccaaaga agttaaagtt ttatatcaca cttcaggagc cataacattt gtgaatgaaa
    10681 caccaagagt aatagagcct gtatatactg cccaatggtc agctacttgg atagcaatga
    10741 ggagagaaaa acgagataga acacatttta aaagaatgag atttccacct ttcgatgatg
    10801 acgaaccacc tctgtcatat gaacagcaca tagaaaatat cgagccactg gatcctataa
    10861 atcttccctt agactctcaa gatgatgaat atgttaaaga ttggctctac gattcccggc
    10921 ccttggagga ggatagtaaa aaggtcaatg gtacatctta taaaaagtgg agttttgatt
    10981 tacctgaaat gtcgaactta tataggttat caacgccact tagagatgaa gtaactgaca
    11041 aaaattacta ttatcttttc gataaaaaat ctttttttaa cggtaaagcc cttaataacg
    11101 caatccctgg aggtcctaaa tttgaacctt tatacccaag agaagaggag gaagattata
    11161 acgaattcaa ctctatagat agagtaatat ttagggttcc tattagaagc gaatataagg
    11221 tagcgtttcc tcatctatat aattcccggc cccgttcagt gcgcatacca tggtataata
    11281 atccagtgtc gtgtattatc cagaacgatg aggagtacga cacgcctgca cttttttttg
    11341 atccatcgct gaacccaata cctcacttta ttgataataa ttcatccctc aacgtatcaa
    11401 atacaaagga aaacggtgat ttcactttac ctgaagattt tgcacctctt ttggctgaag
    11461 aagaagaact tattttgcca aatacaaaag atgcgatgtc gttatatcat tcaccttttc
    11521 catttaatag aactaaagga aaaatggtga gagcacaaga tgttgccctg gctaaaaaat
    11581 ggtttttgca acatcctgac gaggagtatc ccgttaaagt taaggtctct taccaaaagc
    11641 tcttgaagaa ctacgtgcta aatgaattgc accctacttt gccaacaaac cacaacaaaa
    11701 ctaagttatt gaagagttta aaaaatacca agtattttca acaaactaca attgattggg
    11761 tagaggcagg actccagcta tgtcgacaag gacacaatat gttaaattta ttaatccaca
    11821 gaaagggcct aacttattta catctcgact acaatttcaa tttaaaacca actaaaacct
    11881 taaccacaaa agaacgtaaa aagtcaagac ttggaaactc atttcatttg atgcgtgaat
    11941 tgctaaaaat gatgaaactg attgtagata cacatgtgca gtttaggtta ggaaatgtag
    12001 atgccttcca gttagcagat ggtatacatt atattttgaa ccacatagga caattgaccg
    12061 gtatttatcg ttataaatat aaagtaatgc atcaaatccg cgcatgtaaa gatctgaaac
    12121 atatcatata ttataagttt aataaaaatt tgggaaaagg acctggttgt ggattttggc
    12181 aacctgcgtg gagagtgtgg ctaaatttct taagaggcac tataccacta ctcgaaagat
    12241 atattggaaa ccttatcaca cgtcaatttg aaggccgttc taatgaaatt gtcaaaacta
    12301 caactaaaca aagacttgac gcctactacg atcttgagtt acgaaactcg gtcatggatg
    12361 acattttaga aatgatgcct gagagtattc gacagaaaaa ggcaagaact atactgcaac
    12421 atttaagtga ggcttggaga tgttggaagg ctaatattcc atgggatgtt cctggtatgc
    12481 cagccccaat caaaaaaata attgaacggt atataaaatc caaagctgat gcctgggtat
    12541 ctgcagctca ttacaatcga gaacgtataa agcgtggagc tcacgtcgaa aagactatgg
    12601 ttaaaaaaaa tctggggcgt ttaacgagac tttggatcaa aaatgagcag gaacgtcaaa
    12661 ggcaaataca gaaaaacggt cccgaaatta ctcccgaaga ggctacgacc atattttctg
    12721 ttatggttga gtggcttgaa tcaaggagct tttcaccaat accatttcca cctttaactt
    12781 acaaaaatga tactaagata ttagttcttg ccctcgaaga cctaaaagat gtctacgcat
    12841 caaaagtacg tttaaatgca tctgaaaggg aagaacttgc gttgatagag gaggcttatg
    12901 ataatcctca cgatactttg aacagaatca aaaagtactt gttgacccag cgagttttta
    12961 agcctgttga tataaccatg atggaaaact atcaaaacat ttcccctgtt tattcagttg
    13021 atcccttgga aaagattacc gatgcatatc ttgatcagta tttatggtac gaagctgacc
    13081 aacgaaagct tttcccaaac tggattaaac caagtgattc agagatacca cctcttctgg
    13141 tatacaagtg gactcagggt ataaacaacc tatctgaaat ttgggatgta tccagaggcc
    13201 aatctgcagt tttgcttgag actactttgg gtgaaatggc cgaaaaaatt gactttactt
    13261 tacttaatag attacttcgc ctgattgtag atcctaatat tgctgactat atcactgcaa
    13321 aaaataatgt tgttatcaac tttaaagata tgagtcacgt caacaaatat ggcttaatac
    13381 gcgggttgaa gttcgcttct ttcatattcc aatattacgg actagttata gatcttttac
    13441 tattgggtca ggaaagggct acagatttgg ctggtccagc taacaatcca aatgaattta
    13501 tgcaattcaa gagcaaagaa gtagaaaagg cacatccgat cagactttac accagatatt
    13561 tagatcgtat atatatgctt tttcactttg aagaagatga gggagaggaa ttgactgatg
    13621 aatacttggc agagaatcca gatccaaact ttgaaaacag catcggatac aataatagaa
    13681 aatgctggcc caaagattcg cgcatgaggc taatacgtca ggacgttaac ctagggcgag
    13741 cagtgttttg ggaaattcaa agtagggtgc caacttcgtt aacgtcaatt aaatgggaaa
    13801 atgcttttgt ttccgtatac agtaaaaaca atccgaactt actcttctct atgtgtggtt
    13861 ttgaagtcag aatattacca agacaaagaa tggaagaggt ggtttctaac gatgaaggtg
    13921 tttgggattt agttgacgaa agaaccaagc aaagaactgc aaaagcatat ttaaaggtat
    13981 ctgaagaaga aatcaaaaaa tttgacagca gaataagagg catattgatg gcctccggtt
    14041 caaccacttt tactaaagtg gccgctaaat ggaacacttc actgatatcc cttttcacat
    14101 actttagaga agccattgtt gccacggagc cgttattaga tatcctggtg aaaggagaaa
    14161 caagaattca aaaccgtgtc aaacttggtt taaattcgaa aatgccaaca agatttccac
    14221 ctgctgtttt ttatacacca aaagaacttg gtgggttggg tatgattagt gcttctcata
    14281 ttttaattcc tgcatctgac ttaagttggt caaaacagac cgatactgga attacgcatt
    14341 ttcgtgctgg tatgacgcac gaggatgaaa aattaatccc aactatcttc cgttacatca
    14401 ctacttggga aaatgaattt ttagactctc aaagggtgtg ggccgagtat gcaacgaaaa
    14461 ggcaggaagc tattcagcag aataggaggt tagcttttga agaacttgaa ggatcgtggg
    14521 atcgtggtat acctcgtatt agcactttat ttcaaagaga tcgacatact ctagcgtatg
    14581 acagaggtca cagaatccgt agggaattta aacagtactc tctagagaga aacagcccct
    14641 tttggtggac aaactcccac catgacggta aattatggaa tttaaatgcc tatagaactg
    14701 atgtgattca agccctagga ggtattgaaa caattttgga acacacttta tttaagggga
    14761 ctggttttaa ttcatgggaa ggtttgtttt gggaaaaggc gtccgggttt gaggatttca
    14821 tgcaatttaa gaagctaact catgctcaaa gaactggttt aagccaaatt ccaaatcgta
    14881 gatttacctt atggtggtca ccaactatta acagagcaaa tgtctatgtc ggtttcttgg
    14941 tgcaattgga tctgactggt atcttcctcc atggtaaaat tcctacgtta aaaatatctt
    15001 taattcaaat attccgtgct catttgtggc aaaaaataca tgaaagtata gtttttgata
    15061 tttgtcaaat tctcgatggt gagctagatg ttttgcaaat tgaatctgtg actaaagaaa
    15121 cagtgcatcc ccgcaaatcg tacaa
//
