LOCUS       sequence-466558-        8260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct for the MitoTRACER genetic reporter to be 
            expressed in the recipient cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Hoover G, Gilbert S, Curley O, Obellianne C, Lin MT, Hixson W, 
            Pierce TW, Andrews JF, Alexeyev MF, Ding Y, Bu P, Behbod F, Medina 
            D, Chang JT, Ayala G, Grelet S
  TITLE     Nerve-to-cancer transfer of mitochondria during cancer metastasis.
  JOURNAL   Nature. 2025 Jun 25. doi: 10.1038/s41586-025-09176-8.
  PUBMED    40562940
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Jun 25. doi: 10.1038/s41586-025-09176-8."
COMMENT     Sequence Label: [#GS15-LV] DsRed GFP TEVp Blast Switch - Recip...
FEATURES             Location/Qualifiers
     source          1..8260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             36..431
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             441..506
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             510..1217
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     LTR             1315..1548
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1620..1741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1657..1676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1711..1730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1936..2524
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2425..2444
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2678..2695
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2812..2833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2848..2853,2854..2871,2872..2878)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2886..2902
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2891..2913
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2910..2926
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2910..2926
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2945..2965
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2947..2965
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2993..3219
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3220..3400
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3447..3572
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4065..4298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4483..4527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4794..4910
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4933..5236
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5237..5440
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5391..5411
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    5442..5460
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5463..5481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5522..5546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5583..5616)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5645..5654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5651..6328
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(5831..5851)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6049..6068
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6243..6266
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(6353..6386)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6437..6446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6443..6445,6446..6448,6449..7159)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6443..6445,6446..6448,6449..7159)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6488..6509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6749..6768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7096..7117
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7190..7214)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7245..7833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7298..7318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7716..7727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7884..7904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7889..8218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8069..8204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        join(8230..8260,1..17)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
       61 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
      121 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
      181 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
      241 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
      301 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
      361 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
      421 tgtgggaggg cggatccgga gtgaaacaga ctttgaattt tgaccttctc aagttggcgg
      481 gagacgtgga gtccaaccca gggcccatgg gagaaagctt gtttaagggg ccgcgtgatt
      541 acaacccgat atcgagcacc atttgtcatt tgacgaatga atctgatggg cacacaacat
      601 cgttgtatgg tattggattt ggtcccttca tcattacaaa caagcacttg tttagaagaa
      661 ataatggaac actgttggtc caatcactac atggtgtatt caaggtcaag aacaccacga
      721 ctttgcaaca acacctcatt gatgggaggg acatgataat tattcgcatg cctaaggatt
      781 tcccaccatt tcctcaaaag ctgaaattta gagagccaca aagggaagag cgcatatgtc
      841 ttgtgacaac caacttccaa actaagagca tgtctagcat ggtgtcagac actagttgca
      901 cattcccttc atctgatggc atattctgga agcattggat tcaaaccaag gatgggcagt
      961 gtggcagtcc attagtatca actagagatg ggttcattgt tggtatacac tcagcatcga
     1021 atttcaccaa cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg gaattgttga
     1081 caaatcagga ggcgcagcag tgggttagtg gttggcgatt aaatgctgac tcagtattgt
     1141 gggggggcca taaagttttc atggtgaaac ctgaagagcc ttttcagcca gttaaggaag
     1201 cgactcaact catgaatcgt cgtcgccgtc gctgaattta aatggtacct ttaagaccaa
     1261 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     1321 ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     1381 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1441 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1501 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     1561 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     1621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1741 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1861 ggccgccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2041 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2581 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2641 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2701 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2761 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2821 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2881 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2941 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     3001 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     3061 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     3121 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     3181 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     3241 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     3301 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     3361 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     3421 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     3481 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     3541 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     3601 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     3661 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     3721 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     3781 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     3841 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     3901 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     3961 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     4021 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     4081 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     4141 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     4201 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     4261 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     4321 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     4381 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     4441 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     4501 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     4561 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     4621 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     4681 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     4741 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     4801 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     4861 gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc
     4921 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     4981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     5041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     5101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     5161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     5221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     5281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     5341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     5401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca gtgatagaga
     5461 tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc tgcagatatc
     5521 aacaagtttg tacaaaaaag caggctttaa aggaaccaat tcagtcgact ggatccggta
     5581 ccataacttc gtatagcata cattatacga agttataatt ctgcagatat ccagcacagt
     5641 ggcggccacc atggatagca ctgagaacgt catcaagccc ttcatgcgct tcaaggtgca
     5701 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggcaagcc
     5761 ctacgagggc acccagaccg ccaagctgca ggtgaccaag ggcggccccc tgcccttcgc
     5821 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     5881 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5941 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcac
     6001 cttcatctac cacgtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca
     6061 gaagaagact ctgggctggg agccctccac cgagcgcctg tacccccgcg acggcgtgct
     6121 gaagggcgag atccacaagg cgctgaagct gaagggcggc ggccactacc tggtggagtt
     6181 caagtcaatc tacatggcca agaagcccgt gaagctgccc ggctactact acgtggactc
     6241 caagctggac atcacctccc acaacgagga ctacaccgtg gtggagcagt acgagcgcgc
     6301 cgaggcccgc caccacctgt tccagtagag ctagctgaat aaggccgctc gaataacttc
     6361 gtatagcata cattatacga agttattcga gtctagaggg cccgttagat ctctcgaggt
     6421 taacgaattc gactacgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     6481 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6541 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6601 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6661 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6721 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6781 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6841 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6901 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6961 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7021 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     7081 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     7141 catggacgag ctgtacaagc gaattcgcgg ccgcactcga gatatctaga cccagctttc
     7201 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt
     7261 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7321 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7381 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7441 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7501 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7561 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7621 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7681 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7741 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7801 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggttagt
     7861 aatgagtttg gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8041 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     8161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8221 gctccccctg ttgacaatta atcatcggca tagtatatcg
//
