LOCUS       sequence-466559-        5697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing Cre recombinase that is used to insert DNA CARGO 
            into RMCE homing locus in Rosa26.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Trotman JB, Abrash EW, Murvin MM, Braceros AK, Li S, Boyson SP, 
            Salcido RT, Cherney RE, Bischoff SR, Kaufmann K, Eberhard QE, Zhang 
            Z, Cowley DO, Calabrese JM
  TITLE     Isogenic comparison of Airn and Xist reveals core principles of 
            Polycomb recruitment by lncRNAs.
  JOURNAL   Mol Cell. 2025 Mar 20;85(6):1117-1133.e14. doi: 
            10.1016/j.molcel.2025.02.014.
  PUBMED    40118040
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.02"; journalName: "Mol Cell"; date: 
            "2025-03-20- 20"; volume: "85"; issue: "6"; pages: "1117-1133.e14"
COMMENT     Sequence Label: pOG-Cre
FEATURES             Location/Qualifiers
     source          1..5697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(36..1064)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(1065..1085)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1125..1144)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(1423..1441)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1486..1689)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1515..1535)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1690..2069
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(2322..2340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2350..2367)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2350..2366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2359..2381)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2508..2963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2595..2614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2805..2826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2989..3093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3094..3162,3163..3954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3312..3331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4125..4713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4614..4633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4867..4884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5001..5022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5037..5042,5043..5060,5061..5067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5075..5091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5080..5102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5099..5115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5099..5115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5134..5154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5136..5154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    5204..5252
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(5370..5389)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tcaaataatt atagcaatca tttacgcgtt aatggctaat cgccatcttc cagcaggcgc
       61 accattgccc ctgtttcact atccaggtta cggatatagt tcatgacaat atttacattg
      121 gtccagccac cagcttgcat gatctccggt attgaaactc cagcgcgggc catatctcgc
      181 gcggctccga cacgggcact gtgtccagac caggccaggt atctctgacc agagtcatcc
      241 ttagcgccgt aaatcaatcg atgagttgct tcaaaaatcc cttccagggc gcgagttgat
      301 agctggctgg tggcagatgg cgcggcaaca ccattttttc tgacccggca aaacaggtag
      361 ttattcggat catcagctac accagagacg gaaatccatc gctcgaccag tttagttacc
      421 cccaggctaa gtgccttctc tacacctgcg gtgctaacca gcgttttcgt tctgccaata
      481 tggattaaca ttctcccacc gtcagtacgt gagatatctt taaccctgat cctggcaatt
      541 tcggctatac gtaacagggt gttataagca atccccagaa atgccagatt acgtatatcc
      601 tggcagcgat cgctattttc catgagtgaa cgaacctggt cgaaatcagt gcgttcgaac
      661 gctagagcct gttttgcacg ttcaccggca tcaacgtttt cttttcggat ccgccgcata
      721 accagtgaaa cagcattgct gtcacttggt cgtggcagcc cggaccgacg atgaagcatg
      781 tttagctggc ccaaatgttg ctggatagtt tttactgcca gaccgcgcgc ctgaagatat
      841 agaagataat cgcgaacatc ttcaggttct gcgggaaacc atttccggtt attcaacttg
      901 caccatgccg cccacgaccg gcaaacggac agaagcattt tccaggtatg ctcagaaaac
      961 gcctggcgat ccctgaacat gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg
     1021 accggtaatg caggcaaatt ttggtgtacg gtcagtaaat tggacacctt cctcttcttc
     1081 ttgggcatgg ccccgggaga tcccggttgg acctgggagt ggacacctgt ggagagaaag
     1141 gcaaagtgga tgtcattgtc actcaagtgt atggccagat cgatctcaag cctgccacac
     1201 ctcaagcttg acaacaaaaa gattgtcttt tctgaccaga tggacgcggc caccctcaaa
     1261 ggcatcaccg cgggccaggt gaatatcaaa tcctcctcgt ttttggaaac tgacaatctt
     1321 agcgcagaag tcatgcccgc ttttgagagg gagtactcac cccaacagct ggccctcgca
     1381 gacagcgaat tctaggaaga tcttcctaga agcttgggtc tccctatagt gagtcgtatt
     1441 aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata
     1501 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     1561 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     1621 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     1681 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     1741 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     1801 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     1861 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     1921 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     1981 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     2041 ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta catcgcgaag
     2101 cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca agccttgcct
     2161 tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac aagcagggag
     2221 cagactctaa atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca
     2281 ctttcaaaag tccgaaagac cgcggtggag ctccaattcg ccctatagtg agtcgtatta
     2341 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2401 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2461 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     2521 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2581 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2641 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2701 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2761 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2821 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2881 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2941 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     3001 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3061 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3121 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3181 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3241 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3301 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3361 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3421 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3481 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3541 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3601 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3661 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3721 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3781 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3841 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4921 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4981 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5041 cactttatgc tcccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     5101 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     5161 aagctgggta ccgggccccc cctcgaggtc gacggatcga tccgaacaaa cgacccaaca
     5221 cccgtgcgtt ttattctgtc tttttattgc cgatcccctc agaagaactc gtcaagaagg
     5281 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     5341 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     5401 tagcggtccg ccacacccag cctcgacaat tccaacctta cccaagagtt cgccaaactc
     5461 agacatcact ttagcaaaac cgcgccgtgc ttcttcctcg gtggcattca tcacgaaatg
     5521 ttcagcacta cgcatacttt tgacaggaaa cgcaacggat attgagtcaa tatcaggcat
     5581 tctatcgctc agctttacag tgacaatgac ggctggcgac tgaatattag tgcttacaga
     5641 cagcactaca tattttccgt cgatgttgaa atcctttctc atatgtcacc ataaata
//
