LOCUS       sequence-466566-        9414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a 
            gRNA targeting Nrl, efficiently reprogramming rod precursors into 
            cone-like cells in the mouse retina..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Zhao C, Cao Y, Ibrahim N, Wang Y, Martemyanov KA
  TITLE     Efficient in vivo labeling of endogenous proteins with SMART 
            delineates retina cellular and synaptic organization.
  JOURNAL   Nat Commun. 2025 Apr 22;16(1):3768. doi: 10.1038/s41467-025-58945-6.
  PUBMED    40263339
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-04-22"; volume: "16(1):3768. doi"; pages: " 
            10.1038/s41467-025-58945-6"
COMMENT     Sequence Label: pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no I...
FEATURES             Location/Qualifiers
     source          1..9414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        271..346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        526..829
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        831..1108
                     /label=chicken beta-actin promoter
     intron          1109..2125
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2049..2071
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2133..2152
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2200..2209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2209..2274
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2281..2301
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2326..4863,4864..4866,4867..5328,5329..5331,5332..5499,
                     5500..5502,5503..6426)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6427..6474
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6490..6543
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6544..6546,6547..7257)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6544..6546,6547..7257)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6847..6866)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7194..7215
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7285..7302)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7291..7498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7543..7561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7629..7733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7734..7802,7803..8594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7952..7971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8765..9353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9254..9273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg gtatggtgtg gagcccaacg gttttagagc tagaaatagc aagttaaaat
      301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga
      361 gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca
      421 gacaaatggc tctagtaacg gccgccagtg tgctggaatt caggctagtt attaatagta
      481 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      541 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      601 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      661 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      721 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      781 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
      841 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
      901 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc
      961 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     1021 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     1081 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     1141 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     1201 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     1261 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1321 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1381 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1441 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     1501 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1561 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1621 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     1681 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     1741 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1801 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     1861 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     1921 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     1981 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     2041 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     2101 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     2161 tcattttggc aaagaattcc tgaattctgc agatccggtg ccaccatgga ctataaggac
     2221 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
     2281 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
     2341 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     2401 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     2461 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     2521 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     2581 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     2641 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     2701 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     2761 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     2821 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     2881 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     2941 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     3001 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     3061 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     3121 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     3181 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     3241 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     3301 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     3361 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     3421 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     3481 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     3541 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     3601 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     3661 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     3721 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     3781 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     3841 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     3901 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     3961 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     4021 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     4081 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     4141 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     4201 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     4261 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     4321 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     4381 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     4441 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     4501 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     4561 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     4621 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     4681 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     4741 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     4801 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     4861 ctggccgacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     4921 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     4981 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     5041 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     5101 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     5161 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     5221 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     5281 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctgc gctggaaagc
     5341 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     5401 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     5461 aagaccgaga ttaccctggc caacggcgag atccggaagg cgcctctgat cgagacaaac
     5521 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     5581 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     5641 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     5701 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     5761 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     5821 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     5881 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     5941 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     6001 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     6061 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     6121 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     6181 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     6241 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     6301 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     6361 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     6421 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggaattc
     6481 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     6541 ccagtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6601 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6661 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6721 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     6781 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6841 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6901 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6961 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     7021 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     7081 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     7141 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7201 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggaa
     7261 ttctaactag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7321 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7381 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7441 gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc
     7501 ggccgcatgc ctgaccaatg cagagtcgcg cgagacgaaa gggcctcgtg atacgcctat
     7561 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     8461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
