LOCUS       sequence-466611-       10784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse mutant Etfdh (AAA4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Papadopoli D, Palia R, Jovanovic P, Tabaries S, Ciccolini E, 
            Sabourin V, Igelmann S, McLaughlan S, Zhan L, Kim H, Chekkal N, 
            Szkop KJ, Bertomeu T, Zeng J, Vassalakis J, Afzali F, Mzoughi S, 
            Guccione E, Tyers M, Avizonis D, Larsson O, Postovit LM, Djuranovic 
            S, Ursini-Siegel J, Siegel PM, Pollak M, Topisirovic I
  TITLE     Mitochondrial ETF insufficiency drives neoplastic growth by 
            selectively optimizing cancer bioenergetics
  JOURNAL   bioRxiv 2024.10.25.620155
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.10.25.620155"
COMMENT     Sequence Label: pLenti-mEtfdh-AAA4
FEATURES             Location/Qualifiers
     source          1..10784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(8..28)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    46..163
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(429..473)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    658..891
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1384..1509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1556..1736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1737..1963)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1991..2011)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1991..2009)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2030..2046)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2030..2046)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2043..2065)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2054..2070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2078..2084,2085..2102,2103..2108))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2123..2144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2261..2278)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2432..3020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2512..2531)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3191..3982,3983..4051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3814..3833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4052..4156)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4182..4637)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4319..4340)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4531..4550
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4764..4786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4778..4795
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4779..4795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4805..4824
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4805..4823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4844..4979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5019..5140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5030..5049)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5084..5103
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5212..5445
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5517..6542)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5517..6542)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6559..7058)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6607..6626)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7000..7023
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    7089..7677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7195..7206
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7604..7624
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    7695..7719
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9589..9613)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(join(9613..10784,1..7))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9622..10560
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9645..9665)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cggagcccaa cttttctata caaagttgat ccgataatca ctagtaaaat tttgaatttt
       61 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
      121 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagctagcg ataccgtcga
      181 gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt
      241 ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
      301 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
      361 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
      421 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
      481 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
      541 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
      601 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
      661 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
      721 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
      781 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
      841 gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact
      901 gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt
      961 tcaattttta ctactttata tttatataat tcacttctcc aattgtccct catatctcct
     1021 cctccaggtc tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     1081 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     1141 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     1201 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     1261 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     1321 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     1381 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     1441 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     1501 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     1561 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     1621 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     1681 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccgttt
     1741 atgtatcgag ctaggcactt aaatacaata tctctgcaat gcggcaattc agtggttcgt
     1801 ccaatccatg tcagacccgt ctgttgcctt cctaataagg cacgatcgta ccaccttact
     1861 tccaccaatc ggcatgcacg gtgctttttc tctccttgta aggcatgttg ctaactcatc
     1921 gttaccatgt tgcaagacta caagagtatt gcataagact acattaagct tgcagctcca
     1981 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2041 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2101 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2161 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2221 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2281 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2341 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2401 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2461 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2521 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2581 gatacctgtc cgcctttctc tcttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2641 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2701 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2761 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2821 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2881 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2941 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3001 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3061 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3121 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3181 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3241 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3301 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3361 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3421 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3481 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3541 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3601 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3661 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3721 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3781 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3841 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3901 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3961 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4021 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     4201 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4261 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4321 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4381 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4441 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4501 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4561 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4621 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     4681 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     4741 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
     4801 cgcgtaatac gactcactat agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct
     4861 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     4921 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     4981 ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg atgagtttgg
     5041 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5101 tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc tctgatattc
     5161 atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga
     5221 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     5281 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     5341 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag
     5401 caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt
     5461 ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtaccctat
     5521 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     5581 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     5641 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     5701 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
     5761 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     5821 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
     5881 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     5941 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     6001 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     6061 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     6121 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     6181 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     6241 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctaaattcc
     6301 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     6361 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     6421 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     6481 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     6541 atggtggcca cgtgaggtcg aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga
     6601 cgtgcgcttt tgaagcgtgc agaatgccgg gcctccggag gaccttcggg cgcccgcccc
     6661 gcccctgagc ccgcccctga gcccgccccc ggacccaccc cttcccagcc tctgagccca
     6721 gaaagcgaag gagcaaagct gctattggcc gctgccccaa aggcctaccc gcttccattg
     6781 ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca tttgtcacgt
     6841 cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag gagtagaagg
     6901 tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg atgtggaatg
     6961 tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca
     7021 tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattcga accgcgggaa
     7081 ttcccgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     7141 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     7201 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     7261 cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag
     7321 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     7381 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     7441 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     7501 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     7561 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     7621 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattatc
     7681 ggaattcgat tatcaccact ttgtacaaga aagctgggtt tacatgccat tgtaagcagg
     7741 ccctcctcct ccttctggga ccacccagtt aatattttga cttgggtctt tgatatcaca
     7801 tgttttacag tgcacacagt tctgagcatt tatctgtaat cgaaatccat caccttgttc
     7861 caaaggaaca aattcataaa ctcctgcagg gcagaatcgc tgctcaggcc catcgtatat
     7921 tgacaggttt ctattaacag gtatgctgtc atcctttaag gttaaatgtg ctggctggtc
     7981 atgttcatga ttagtaccac tcaaagccac agaggataag aggtcaaaac tgatctgtcc
     8041 atcaggtttc ggatactcaa tgggtgtaca atccttggct ggtttgagct gatcagagtc
     8101 tgagccttta tgttttagag tccatggctc cattcctctc aatatccagt aaaatatccc
     8161 agtgtaaatc atccctccat atactcccag tatcccgtgg caagatggcc ttatatttcg
     8221 aacagcgtgt agctctttcc atacccagga ttgcttcaaa ttgtcctcat attcagttac
     8281 atggagtcct gttgtctttg actggagatt ttcactagtt agttgcttaa aaattgattc
     8341 tgctgccaaa cttccacttt tcattgccgt gtgggtacct ttgatcttgg ggacattcat
     8401 gaaaccagga ctacaaccaa ttagtaagcc accaggaaaa gtgagttttg gtatggactg
     8461 caagccgcct tcattgagcg ctcgggctcc gtaggctatc ctcttcccgc cttccagggt
     8521 tggctggatg ctgggatgat gcttccacct ctggaactct cggaatggac tcaggtacgg
     8581 attctgatag tcgaggccaa ccacaaagcc aacagctact agaggttcac cttcatttaa
     8641 gtggtaaagg aaagagcctc cataagtatg tctgtccaag ggccaaccaa cagtgtgatc
     8701 tactcttcca ggtttccact tcttttcatc aataatccat aactccttca aaccaattcc
     8761 gtaagtctgt gcatcacagc tggccctcag atcaaatttc ttataaagct gcttggctag
     8821 gtgtccatgg caaccctctg caaagactgt gactttggca tgcagttcca gtcctctctc
     8881 aaatgttgtc tttggtgcac catccttttg tatgcctaca tcattagtgg cgattccttt
     8941 tacactacca tcttcatgat aaaggacctc agcagcagca tacccaggat acacttcgac
     9001 tccaagagcc tctgcctgtt ctcccatcca gctcaccaga tgtcctaggc gtacgatgta
     9061 attgccatga ttgttcatcg gaagacctgg aagaattggc acaggaattc tgtgtttctc
     9121 tgttaaaatg gcaaacctgt cttctgttac aggagtatta agtggagccc ctttctcctt
     9181 ccagtctggg aagagctctt tgaaggcagc tggatcaaga caagcccctg agagcgtgtg
     9241 tgctccgatc tgagcagctt tctccaccag acacacacgg atgtccttcc cctgttcagc
     9301 agccagctgc tttagccgaa tagctgcaga gagtcctgct gggccggcac caactatgac
     9361 cacatctgct tcttctgcaa acctctccat attcactcct tcccatcttt tgtctttctc
     9421 ccggggatga acagtgtagt gagtggtaat ctgaggtttt ttttttttta cagcagaggt
     9481 tgaagaacat cttggcgcgc atagtggtag acacttctta atttttaagg catgaaacca
     9541 atggtaggca gggcaggaca gcttggttag tcgcaccaac atggtggcag cctgcttttt
     9601 tgtacaaact tgtcacgaca cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg
     9661 cttgagaatg aaccaagatc caaactcaaa aagggcaaat tccaaggaga attacatcaa
     9721 gtgccaagct ggcctaactt cagtctccac ccactcagtg tggggaaact ccatcgcata
     9781 aaacccctcc ccccaaccta aagacgacgt actccaaaag ctcgagaact aatcgaggtg
     9841 cctggacggc gcccggtact ccgtggagtc acatgaagcg acggctgagg acggaaaggc
     9901 ccttttcctt tgtgtgggtg actcacccgc ccgctctccc gagcgccgcg tcctccattt
     9961 tgagctccct gcagcagggc cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga
    10021 ccgggccagc cttgccgccc agggcggggc gatacacggc ggcgcgagac caggcaccag
    10081 agcaggccgg ccagcttgag actacccccg tccgattctc ggtggccgcg ctcgcaggcc
    10141 ccgcctcgcc gaacatgtgc gctgggacgc acgggccccg tcgccgcccg cggccccaaa
    10201 aaccgaaata ccagtgtgca gatcttggcc cgcatttaca agactatctt gccagaaaaa
    10261 aagcgtcgca gcaggtcatc aaaaatttta aatggctaga gacttatcga aagcagcgag
    10321 acaggcgcga aggtgccacc agattcgcac gcggcggccc cagcgcccag gccaggcctc
    10381 aactcaagca cgaggcgaag gggctcctta agcgcaaggc ctcgaactct cccacccact
    10441 tccaacccga agctcgggat caagaatcac gtactgcagc caggtggaag taattcaagg
    10501 cacgcaaggg ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac
    10561 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
    10621 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
    10681 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt
    10741 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcac
//
