LOCUS       sequence-466638-       10190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin FRET tension sensor with coiled-coil S7 domain no vinculin 
            force control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, 
            Salaita K
  TITLE     Digital and Tunable Genetically Encoded Tension Sensors Based on 
            Engineered Coiled-Coils.
  JOURNAL   Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 
            10.1002/anie.202407359.
  PUBMED    39805005
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359."
COMMENT     Sequence Label: Vinculin-Clover-CC-S7-mRuby2
FEATURES             Location/Qualifiers
     source          1..10190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     864..883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4170..4191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4778..4799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5782..5799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5788..6012
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6058..6486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6145..6164)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6355..6376
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6495..6515)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6500..6829
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6680..6815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6742..6761
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6896..7690
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6950..6969)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7560..7579
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7864..7985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7901..7920)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7955..7974
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8034..8050)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8034..8050)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8047..8069)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8058..8074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8082..8088,8089..8106,8107..8112))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8127..8148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8265..8282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8436..9024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8516..8535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9195..9986,9987..10055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9818..9837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10056..10160)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      901 cgtttaaact taagcttgcc atgcccgtct tccacacgcg caccatcgag agcatcttgg
      961 agcccgtggc tcagcagatc tcccacctgg tcatcatgca cgaggagggg gaggtagacg
     1021 gcaaggccat cccggacctc accgcccccg tgtcggccgt gcaggccgct gtcagcaacc
     1081 tggtgcgggt tggaaaagaa actgtgcaga caacagaaga ccagatcttg aaaagggata
     1141 tgccaccagc attcatcaaa gtagagaatg cctgcaccaa gctcgttcga gcagcccaga
     1201 tgctgcaagc agatccttat tcagtaccag ctcgtgacta cctaattgat ggatcaagag
     1261 gcatcctttc tggaacatca gacttacttc tgacatttga tgaagcggag gtccgtaaaa
     1321 tcatccgtgt ctgcaaagga atattggaat atctgactgt ggcagaagta gtagagacta
     1381 tggaggattt ggtgacatat acaaagaatc tagggccagg aatgacaaag atggcgaaaa
     1441 tgatcgatga gagacaacag gaattaactc atcaggaaca tagggttatg ctggtgaact
     1501 ccatgaatac tgtgaaggag ctattgcctg tacttatttc agctatgaag atctttgtaa
     1561 ccacaaaaaa cactaaaagc cagggaatag aagaggcctt gaaaaatcgc aatttcacag
     1621 tagagaaaat gagtgctgag ataaatgaaa taatccgtgt attacaactc acttcctggg
     1681 atgaagatgc ctgggccagc aaggacactg aagccatgaa aagagcttta gccctaatag
     1741 attcaaagat gaaccaggca aaaggctggc tgagagatcc aaacgcacct ccaggggatg
     1801 ctggtgagca agcaataagg cagatccttg atgaagctgg aaaagcagga gaattgtgtg
     1861 caggcaaaga acgcagggag attctgggaa catgcaaaac tttgggccaa atgactgatc
     1921 aacttgctga tctccgagct agaggacagg gtgctacacc gatggcaatg cagaaggcac
     1981 agcaggtgtc acaaggcctg gatttgctca ctgcaaaagt ggagaatgca gcccggaaat
     2041 tggaggccat gacaaactct aagcaggcta ttgcaaagaa gattgatgct gctcagaact
     2101 ggcttgcgga tcccaacggc ggaagtgaag gagaagaaca cattcgagga attatgtctg
     2161 aagcaaggaa agttgcagaa ttgtgtgagg agcctaaaga aagagatgat atccttcgct
     2221 ccttggggga aatctctgct ctgacagcta agctgtcaga tctgcgacga catgggaaag
     2281 gcgactctcc tgaggcccgt gcattggcca agcaaatagc tacatcactt cagaacttac
     2341 agtccaaaac aaacagagct gtagcaaata ctaggccagt taaagctgct gtccatttgg
     2401 agggcaagat tgagcaagcc cagaggtgga tagataatcc tacagttgat gatcggggag
     2461 taggccaggc agcaattcgg ggtttggttg cagaaggtcg tcgtctagcc aatgtcatga
     2521 tgggacctta tcgtcaggac ctgcttgcca aatgtgaccg tgtagaccag ctggctgctc
     2581 agcttgctga ccttgcagca agaggggaag gagagtctcc tcaggctagg gcaattgctg
     2641 ctcagcttca ggactccctg aaggatctca aagcacggat gcaagaagca atgacccagg
     2701 aggtgtctga tgttttcagt gacactacaa cccctattaa gttgttagca gtagcagcca
     2761 ctgctccttc tgatactccc aatagagaag aggtgtttga agaaagagca gcaaattttg
     2821 aaaaccatgc tgctagactg ggagcaacag cagaaaaagc agctgcagtt ggaactgcta
     2881 ataaaactac tgtggaaggc attcaggcaa cagtcaaatc tgcaagggag cttacaccac
     2941 aggtagtatc ggctgctcga atcctcctga gaaatcctgg aaatcaagct gcttatgagc
     3001 attttgagac aatgaaaaac caatggattg ataatgtaga aaagatgaca gggctggtgg
     3061 atgaggccat cgataccaag tctctgttgg atgcatcaga agaggctatt aagaaggatc
     3121 ttgataaatg taaagttgca atggccaata tgcaacctca gatgctggta gctggagcca
     3181 ccagcattgc tagacgagcg aaccgcatcc tgcttgtggc aaaacgggag gttgaaaatt
     3241 cagaagaccc taaattcagg gaggctgtta aagcagcttc tgatgagctg agcaaaacca
     3301 tatcaccgat ggtaatggat gctaaagctg tagcaggaaa tatctctgat cctggtttgc
     3361 agaagagttt cttggattct ggatacagga ttctgggagc tgtggccaaa gtcagagaag
     3421 cctttcagcc tcaggagcca gactttcccc ctcctcctcc tgacctagag catctccatc
     3481 tgactgatga gcttgctcct ccaaaaccac cacttccaga aggtgaggtt cccccaccca
     3541 gaccaccacc ccctgaagaa aaagatgagg agttcccaga gcagaaagca ggagaagcta
     3601 ttaatcagcc catgatgatg gctgctaggc agttgcatga cgaggctcgg aaatggtcta
     3661 gcaagggtaa cgacatcatt gctgctgcta aacgaatggc gctgctcatg gctgagatgt
     3721 cacgcctggt gcgaggaggc agcggaaaca agcgtgccct catccagtgt gcaaaagata
     3781 ttgctaaggc atcggatgaa gtcactcgat tggccaaaga ggtggccaag caatgtactg
     3841 ataaacgcat tagaacaaac ctcttacagg tctgtgagcg aatcccaacc atcagcacgc
     3901 agctcaaaat tctttccaca gtgaaagcta ccatgctggg caggactaac atcagcgatg
     3961 aagaatcaga acaggcaact gagatgttgg ttcataacgc ccagaacctc atgcagtctg
     4021 tgaaggaaac tgtgagagaa gctgaagcag catccattaa gataagaaca gatgccggat
     4081 tcactctgcg ctgggtcaga aagaccccat ggtatcagga attcatggtg agcaagggcg
     4141 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     4201 acaagttcag cgtccgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga
     4261 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     4321 gctacggcgt ggcctgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     4381 agtccgccat gcccgaaggc tacgtccagg agcgcaccat ctctttcaag gacgacggta
     4441 cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4501 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4561 tcaacagcca caacgtctat atcacggccg acaagcagaa gaacggcatc aaggctaact
     4621 tcaagatccg ccacaacgtt gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4681 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agccatcagt
     4741 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4801 ccgccgccgg gtccggaggg gggtctgcac aatcagagaa agagtcacaa gccagcgaga
     4861 aagaaaatgc ccagtccgaa tgggagagcc aggcaagtga aaaggaaagc gcccaagggg
     4921 gtagtggtgg ctctcaggca tctaagaaga agtccgcaca atctaaatgg aaaagtcagg
     4981 caaataagaa gaaaagtgcc caaagcaaga aaaaaagcca ggccggaggt agtaccggta
     5041 aaggtgaaga attaatcaag gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca
     5101 acggccacca attcaaatgc acaggtgaag gagaaggcaa tccgtacatg ggaactcaaa
     5161 ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac attcttgcca
     5221 cgtcgttcat gtatggcagc cgtactttta tcaagtaccc gaaaggcatt cctgatttct
     5281 ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac gaagatggtg
     5341 gagtcgtcac cgtcatgcag gacaccagcc ttgaggatgg ctgtctcgtt taccacgtcc
     5401 aagtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag accaagggtt
     5461 gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagggga tacactcata
     5521 tggcactgaa agttgatggt ggtggccatc tgtcttgctc tttcgtaaca acttacaggt
     5581 caaaaaagac cgtcgggaac atcaagatgc ccggtatcca tgccgttgat caccgcctgg
     5641 aaaggttaga ggaaagtgac aatgaaatgt tcgtagtaca acgcgaacac gcagttgcca
     5701 agttcgccgg gcttggtggt gggatggacg agctgtacaa gtaaatcgat tctagagggc
     5761 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5821 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5881 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5941 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6001 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6061 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6121 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6181 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6241 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6301 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6361 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6421 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6481 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6541 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6781 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6841 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6901 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6961 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     7021 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     7081 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7141 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7201 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7261 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7321 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7381 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7441 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7501 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7561 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7621 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7681 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7741 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7801 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7861 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7981 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8041 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8101 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8161 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8221 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8281 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8401 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9181 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9301 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9481 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9781 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10081 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10141 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
//
