LOCUS       sequence-466652-        4324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  codon-optimized mStayGold with synthetic introns for the expression 
            in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ko S, Mizumoto K
  TITLE     Comparison among bright green fluorescent proteins in C. elegans.
  JOURNAL   MicroPubl Biol. 2025 Jan 18;2025:10.17912/micropub.biology.001447. 
            doi: 10.17912/micropub.biology.001447. eCollection 2025.
  PUBMED    39897165
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2025-01-18"; volume: 
            "2025:10.17912/micropub.biology.001447. doi"; pages: " 
            10.17912/micropub.biology.001447. eCollection 2025"
COMMENT     Sequence Label: pSM-mStayGold
FEATURES             Location/Qualifiers
     source          1..4324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1894..1916)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2016..2035
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2117..2135)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2203..2307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2308..2376,2377..3168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2526..2545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3339..3927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3828..3847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4081..4098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4215..4236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4251..4256,4257..4274,4275..4281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4289..4305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4294..4316
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4313..4324,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4313..4324,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccactgact
       61 gggcgcgcct ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
      121 atactaacat aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt
      181 agaaaaaatg gtttcaacgg gagaagaact tttcacaggt gtcgtgccat tcaagttcca
      241 actcaaggga accatcaacg gaaagtcgtt caccgttgaa ggagagggag agggaaattc
      301 acatgaggga tcacacaagg gaaaatacgt gtgcacatcg ggaaagctcc caatgtcatg
      361 ggctgctctt ggaacatcat tcggatacgg aatgaagtac tacacgaagt acccgtcggg
      421 actcaaggta agtttaaaca tatatatact aactaaccct gattatttaa attttcagaa
      481 ctggttccac gaagtgatgc cagagggatt cacatacgat cgacacatcc agtacaaggg
      541 agatggatcg attcacgcta agcaccagca tttcatgaag aacggaacct accacaacat
      601 cgtcgagttc accggacagg atttcaaggt aagtttaaac agttcggtac taactaacca
      661 tacatattta aattttcagg agaactcgcc ggtcctcacg ggagatatgg atgtttcact
      721 cccaaacgag gtgcagcaca ttccaatcga tgatggtgtt gagtgcacag tgacccttca
      781 atacccactc ctctcggatg agtcaaaatg cgttgaggcc taccagaaca cgatcattaa
      841 gccactccac aatcagccag ctccagatgt tccattccac tggattcgaa agcagtacac
      901 ccagtcgaag gatgatacag aggaacgtga tcacatcatc cagtcagaaa cactcgaagc
      961 ccatctctga cattcgtaga attccaactg agcgccggtc gctaccatta ccaacttgtc
     1021 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     1081 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     1141 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     1201 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     1261 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     1321 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     1381 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     1441 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     1501 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     1561 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     1621 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     1681 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     1741 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     1801 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
     1861 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     1921 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1981 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     2041 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2101 gcggccttaa ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2161 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2221 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2281 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2341 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2401 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2461 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2521 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2581 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2641 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2701 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2761 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2821 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2881 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2941 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3001 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3061 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3121 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3181 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3241 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3301 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3361 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3421 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3481 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3541 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3601 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3661 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3721 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3781 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3841 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3901 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3961 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4021 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4081 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4141 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4201 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     4261 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     4321 agct
//
