LOCUS       sequence-466684-        8828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged STE3 (C-terminally tagged, optimized for yeast 
            expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-TEF1pr-STE3-GFP
FEATURES             Location/Qualifiers
     source          1..8828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     263..291
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(1807..2205)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(2223..2243)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2223..2241)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2262..2278)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2262..2278)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2275..2297)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2286..2302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2310..2316,2317..2334,2335..2340))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2493..2510)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2664..3252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2744..2763)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3423..4214,4215..4283))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4046..4065
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4284..4388)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4425..4928
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     4970..4988
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5026..5048)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5148..5167
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(5661..6755)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6756..7163)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(7464..7919)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7601..7622)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7813..7832
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8045..8067
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8059..8076
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8060..8076
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8086..8105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8086..8104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(8123..8370)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     8336..8354
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(8436..8828,1..321))
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(8476..8496)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cttgtagttc ccgtcatctt tgaaaaatat agttctttcc tgtacataac cttcgggcat
       61 ggcactcttg aaaaagtcat gccgtttcat atgatctggg tatcttgaaa agcattgaac
      121 accataggtg aaagtagtga caagtgttgg ccatggaaca ggtagttttc cagtagtgca
      181 aataaattta agggtaagtt ttccgtatgt tgcatcgcct tcaccctctc cactgacaga
      241 aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
      301 aagttcttct cctttactca ttgctccggc tcctgcgtcg acggtatcga taagcttgat
      361 atcgaattcc tgcagcccgg gagggcctgc agtattttct gaactatgtt cgtttgagct
      421 tgaattctct tcttttgaag ctggagagta gcaaagactt tccccttcaa aattggagct
      481 tgcagaatta tgaagctttt ctgaataatc gagagtaact tttgagaatc ctccaagtga
      541 tatttcgtcg tcaaagctct gtccttgcgt gacgtaaggg atgtgcctac tttcttttag
      601 gtctatttca tcaagaatat catctgtttc tttatcagca aacaaatatt ttttgctttt
      661 attttgagat tttctaggat catctggaat cctatagtcg acatagaaat gcgcttgtga
      721 tattggtgta cctacacagg gagaatagtt ttctgtacac gtggagatat agttctcaga
      781 gtctgtagaa aatgggttac ggctctcttt gcgtgaggac agcttgttta gcaatatgcc
      841 tactcgtttt tccttattct tatcaatgaa tcttttccac atgtcaagta caaatcctag
      901 tttgatggaa cgcaggaatt tagagtacat atgcaaagca tcagaaccta agccaaagat
      961 tagaaatact aggtaagaca tcaaaacata aagccatata ttataaattg gtctgccagg
     1021 gtcaaattta ataatggtat tccagatggt gctggaatgg gtatttttaa aagtatagtg
     1081 tccttctacc tgctgtaaat cttgaacaaa ggtgtaaaca gaaaaaggga acatgactaa
     1141 aataataatg aaacagaata tcaacagcct tgcgaacctt gtcaggttta aacctgaatt
     1201 ggtacagtgt aaaatatccc taacgtcctt gcgtttttta taaaacacga acagtactaa
     1261 ggtggcataa acagcgccca caaatgacca tataagcatc cacatggtat acaaaacagt
     1321 ggtaatccat gtcggagaca ataagttttg gcaaccgttg taacgagcga taccatatct
     1381 aaatacttgt aacagatatg aaaatcccat gaccatgaca ggtgtgaaca agctaatcac
     1441 aaggtccttg acgattttcg tccatgatga aagatccggt aaaacactat ctgccttcaa
     1501 aattgtatgc aagttgtaaa tgatgttggt aacggcacat gatatgccaa tattcgcacc
     1561 aacctgcaac ttgatgacaa tatcacacca acctttacca tcccatctcg tgaggaaatc
     1621 gtcgtcactc catattgccg catctacaat acacgttaaa ttcattgtaa gaagccatgt
     1681 tatcaaaatg attgctggaa tattcttggt atgtgaatgc catgctaaag ggggagctaa
     1741 tagtatcaca gctagcaaac aaagccctat tattgctgac ttgtatgaca tactagttct
     1801 agaaaactta gattagattg ctatgctttc tttctaatga gcaagaagta aaaaaagttg
     1861 taatagaaca agaaaaatga aactgaaact tgagaaattg aagaccgttt attaacttaa
     1921 atatcaatgg gaggtcatcg aaagagaaaa aaatcaaaaa aaaaattttc aagaaaaaga
     1981 aacgtgataa aaatttttat tgcctttttc gacgaagaaa aagaaacgag gcggtctctt
     2041 ttttcttttc caaaccttta gtacgggtaa ttaacgacac cctagaggaa gaaagagggg
     2101 aaatttagta tgctgtgctt gggtgttttg aagtggtacg gcgatgcgcg gagtccgaga
     2161 aaatctggaa gagtaaaaaa ggagtagaaa cattttgaag ctatgagctc cagcttttgt
     2221 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2281 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2341 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2401 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2461 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2521 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2581 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2641 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2761 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2821 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2881 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3061 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3121 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3181 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3241 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3301 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3361 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3421 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3481 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3541 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3601 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3661 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3721 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3781 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3841 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3901 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3961 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4021 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4081 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4141 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4201 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4261 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4321 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4381 gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa
     4441 ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa
     4501 gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc
     4561 caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt
     4621 catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg
     4681 ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt
     4741 acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc
     4801 ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac
     4861 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
     4921 aagcgatccg tcctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4981 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5041 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5101 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     5161 gattgtactg agagtgcacc atatcgacta cgtcgttaag gccgtttctg acagagtaaa
     5221 attcttgagg gaactttcac cattatggga aatggttcaa gaaggtattg acttaaactc
     5281 catcaaatgg tcaggtcatt gagtgttttt tatttgttgt attttttttt ttttagagaa
     5341 aatcctccaa tatataaatt aggaatcata gtttcatgat tttctgttac acctaacttt
     5401 ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc
     5461 acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc
     5521 ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca
     5581 ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat
     5641 cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca
     5701 ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa
     5761 acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc
     5821 aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct
     5881 ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag
     5941 gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag
     6001 atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta
     6061 actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt agggaattcg
     6121 ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct
     6181 ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt
     6241 cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca
     6301 ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg
     6361 aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat
     6421 gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa
     6481 ccttgttcag gtctaacact accggtaccc catttaggac cacccacagc acctaacaaa
     6541 acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca
     6601 tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca
     6661 tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca
     6721 cctggcaaaa cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata
     6781 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     6841 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     6901 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcgctctc
     6961 acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg
     7021 aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt
     7081 tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac
     7141 gatacctgag tattcccaca gttaactgcg gtcaagatat ttcttgaatc aggcgcctta
     7201 gaccgctcgg ccaaacaacc aattacttgt tgagaaatag agtataatta tcctataaat
     7261 ataacgtttt tgaacacaca tgaacaagga agtacaggac aattgatttt gaagagaatg
     7321 tggattttga tgtaattgtt gggattccat ttttaataag gcaataatat taggtatgta
     7381 gatatactag aagttctcct cgaccggtcg atatgcggtg tgaaataccg cacagatgcg
     7441 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     7501 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7561 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7621 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7681 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7741 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7801 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7861 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7921 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     7981 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     8041 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     8101 tagggcgaat tgggtaccgg ccgcaaatta aagccttcga gcgtcccaaa accttctcaa
     8161 gcaaggtttt cagtataatg ttacatgcgt acacgcgtct gtacagaaaa aaaagaaaaa
     8221 tttgaaatat aaataacgtt cttaatacta acataactat aaaaaaataa atagggacct
     8281 agacttcagg ttgtctaact ccttcctttt cggttagagc ggatgtgggg ggagggcgtg
     8341 aatgtaagcg tgacataact aattacatga ctcgagcgct cgagtcacta tagggcgaat
     8401 tggagctcca ccgcggtggc ggccgctcta gattatttgt atagttcatc catgccatgt
     8461 gtaatcccag cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga
     8521 tctttcgaaa gggcagattg tgtggacagg taatggttgt ctggtaaaag gacagggcca
     8581 tcgccaattg gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg
     8641 ttgtgtctaa ttttgaagtt ggctttgatt ccattctttt gtttgtctgc catgatgtat
     8701 acattgtgtg agttatagtt gtattccaat ttgtgtccaa gaatgtttcc atcttcttta
     8761 aaatcaatac cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca
     8821 gcacgtgt
//
