LOCUS       sequence-466699-        5594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  20X MYC tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5594)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-20XMYC
FEATURES             Location/Qualifiers
     source          1..5594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1384..1403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             1637..1666
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1679..1708
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1721..1750
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1763..1792
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1805..1834
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1865..1894
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1907..1936
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1949..1978
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1991..2020
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2033..2062
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2093..2122
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2135..2164
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2177..2206
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2219..2248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2261..2290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2321..2350
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2363..2392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2405..2434
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2447..2476
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2489..2518
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3418..3434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3418..3434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3431..3453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3442..3458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3466..3472,3473..3490,3491..3496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3511..3532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3649..3666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3820..4408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3900..3919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4579..5394)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(4579..5394)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5302..5321
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(5395..5499)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaagtcat acatacacaa
      421 aaggacacgc acccacacag acccatcata gaaaccaatt tgaaattctg aatcctgctt
      481 gacgctcgtt aataaatgcc tggagcagtt tttaatgatt gctgagcttc tgtcactcat
      541 cccgtaaaat gtgtttccgg atttttgcag actgtgttca tgaactggac ggtcgccgtg
      601 gagagccatg tggactcgtc cttcttctgg ggctatctgg tgacgcagat tcccggcggc
      661 ttcatcgcct ccaagttccc ggccaacaag atattcggac tgtccatcgt gagctccgcc
      721 acgctgcatc tcttcgtgcc attcgccatg accctgatgc acggtcatgt ggtgatttgc
      781 gtgagggtcc tgcaaggact cttcgaggta tgtagcccaa ccaacctaac aacttgccca
      841 attcgatttt gccacagatc aaagccgggg attacggctg cgtgatgttg cgaaaatggg
      901 gagggttaga ttttgcttta tgataaagca aatatgtgaa atggaaacat gtcctaagcc
      961 ctcaaaggga tgggggtcct gggagggcag gaaaaaataa caaaagcatt tattttaatg
     1021 cgatttcttt gtgccaagga ctgggaagta tgtcattttt cttcgaatcg gaagggagag
     1081 aatgatactt tgtaaaaagg atgtttgtct attcgagttc cgaagtaact atattttaga
     1141 tatatatttt taatttcaca aagcacattt tcttaagata ctataactgc atgagactaa
     1201 gtgcagagtt tcattaagga ataactaatt ccctaatgaa actcgcgtcg cgccacttca
     1261 acgctcgatg ggagcgtcag cgtcgcgcca cttcaacgct cgatgggagc gtcattggtg
     1321 ggcggggtaa ccgtcgaaat cagtgtttag cccgggcagc atttttttca ctgcattcta
     1381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccca aactcatcaa
     1441 tgtatcttat catgtctgga tcgagtttca ttaaggaata actaattccc taatgaaact
     1501 cttggaatat aggacaatat cctaattgac ttatttaaat aatccattcc tgcaggtgcc
     1561 gtcctacgat ccgcagggat accagcagca gggctccggc ggctggcgcg cccttaagct
     1621 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     1681 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     1741 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     1801 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg agggctccgg
     1861 cggcgaacag aaacttatct cagaggagga tcttggctcc ggcggcgagc aaaaactaat
     1921 atcggaggaa gacctcggtt caggagggga acaaaagctc atttctgaag aggacctggg
     1981 atctgggggt gagcaaaagc tcatatcgga agaagacctc ggatctggtg gagaacagaa
     2041 actgatttcc gaagaggatc ttggttcggg gggggtcgag ggctccggcg gcgaacagaa
     2101 acttatctca gaggaggatc ttggctccgg cggcgagcaa aaactaatat cggaggaaga
     2161 cctcggttca ggaggggaac aaaagctcat ttctgaagag gacctgggat ctgggggtga
     2221 gcaaaagctc atatcggaag aagacctcgg atctggtgga gaacagaaac tgatttccga
     2281 agaggatctt ggttcggggg gggtcgaggg ctccggcggc gaacagaaac ttatctcaga
     2341 ggaggatctt ggctccggcg gcgagcaaaa actaatatcg gaggaagacc tcggttcagg
     2401 aggggaacaa aagctcattt ctgaagagga cctgggatct gggggtgagc aaaagctcat
     2461 atcggaagaa gacctcggat ctggtggaga acagaaactg atttccgaag aggatcttgg
     2521 ttcggggggg gtcgacggat ccgcggccgc ataatgggtt cgcgataggt cactgatata
     2581 tatcatagct tttagttgta gtcggaaatc gtttgacgtt tattgtttgt tctcggtgtg
     2641 ccgttaaagt tttcccccct ttttctctct atttgttgag ttccatcgat ggacggatcc
     2701 gtggaccttt cagaagtata taaatatata actaaagagt aacgaatttg tcagcgaata
     2761 aggggattcc acatgccaca ctaacacaat gaaactatgt acctaacaga gaaactacat
     2821 atgcatacac agcaataccg ataaccacta ccaataccat tagcccacat agacccaaac
     2881 tggcctaatc gatctataaa gcatatatag tatatactat tagggtctcg cttacgcgaa
     2941 tttctattta caagctccga aatcgaaact gcaacacaag atagggaaag aactggaaat
     3001 atatactcaa cacttgtgta tatcataaat cacataatcc acacaaagcc tatgataatt
     3061 atatttatat cggtataatc gtatatacat atatagccaa agattgttgt tgaactgcga
     3121 actgcaagtt ttattaccgt tgatgatatt atcttctgtg taaagagaac attttaatca
     3181 aacccaagca attgttgatg ttcggcacga atgataacca taaaaagaac tagctaatca
     3241 aaagtcatat gaatactctg ttaactaatt actgtttatg tttgttgata ttatttaatt
     3301 cattgatatt aatgcaccgt atgcttttgt gtttaactaa catgagcaag gcatgatgta
     3361 ggtactaaaa ataatactta agtagatagc cgtagaagct tggctcgatc gagcatggtc
     3421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4261 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4561 gtaaacttgg tctgacagtt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     4621 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     4681 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     4741 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     4801 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     4861 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     4921 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     4981 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     5041 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccag ggatcgcagt
     5101 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     5161 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     5221 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     5281 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     5341 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt
     5401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5521 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata agcgtatcac
     5581 gaggcccttt cgtc
//
