LOCUS       sequence-466703-        9290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  80X OLLAS tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9290)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-80XOLLAS
FEATURES             Location/Qualifiers
     source          1..9290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1384..1403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             1637..1678
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1691..1732
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1745..1786
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1799..1840
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1853..1894
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1925..1966
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1979..2020
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2033..2074
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2087..2128
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2141..2182
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2213..2254
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2267..2308
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2321..2362
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2375..2416
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2429..2470
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2501..2542
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2555..2596
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2609..2650
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2663..2704
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2717..2758
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2789..2830
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2843..2884
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2897..2938
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             2951..2992
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3005..3046
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3077..3118
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3131..3172
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3185..3226
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3239..3280
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3293..3334
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3365..3406
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3419..3460
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3473..3514
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3527..3568
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3581..3622
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3653..3694
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3707..3748
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3761..3802
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3815..3856
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3869..3910
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3941..3982
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             3995..4036
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4049..4090
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4103..4144
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4157..4198
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4229..4270
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4283..4324
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4337..4378
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4391..4432
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4445..4486
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4517..4558
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4571..4612
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4625..4666
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4679..4720
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4733..4774
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4805..4846
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4859..4900
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4913..4954
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4967..5008
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5021..5062
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5093..5134
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5147..5188
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5201..5242
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5255..5296
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5309..5350
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5381..5422
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5435..5476
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5489..5530
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5543..5584
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5597..5638
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5669..5710
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5723..5764
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5777..5818
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5831..5872
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5885..5926
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5957..5998
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6011..6052
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6065..6106
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6119..6160
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6173..6214
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     primer_bind     complement(7114..7130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7114..7130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7127..7149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7138..7154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7162..7168,7169..7186,7187..7192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7207..7228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7345..7362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7516..8104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7596..7615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8275..9090)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8275..9090)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8998..9017
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(9091..9195)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaagtcat acatacacaa
      421 aaggacacgc acccacacag acccatcata gaaaccaatt tgaaattctg aatcctgctt
      481 gacgctcgtt aataaatgcc tggagcagtt tttaatgatt gctgagcttc tgtcactcat
      541 cccgtaaaat gtgtttccgg atttttgcag actgtgttca tgaactggac ggtcgccgtg
      601 gagagccatg tggactcgtc cttcttctgg ggctatctgg tgacgcagat tcccggcggc
      661 ttcatcgcct ccaagttccc ggccaacaag atattcggac tgtccatcgt gagctccgcc
      721 acgctgcatc tcttcgtgcc attcgccatg accctgatgc acggtcatgt ggtgatttgc
      781 gtgagggtcc tgcaaggact cttcgaggta tgtagcccaa ccaacctaac aacttgccca
      841 attcgatttt gccacagatc aaagccgggg attacggctg cgtgatgttg cgaaaatggg
      901 gagggttaga ttttgcttta tgataaagca aatatgtgaa atggaaacat gtcctaagcc
      961 ctcaaaggga tgggggtcct gggagggcag gaaaaaataa caaaagcatt tattttaatg
     1021 cgatttcttt gtgccaagga ctgggaagta tgtcattttt cttcgaatcg gaagggagag
     1081 aatgatactt tgtaaaaagg atgtttgtct attcgagttc cgaagtaact atattttaga
     1141 tatatatttt taatttcaca aagcacattt tcttaagata ctataactgc atgagactaa
     1201 gtgcagagtt tcattaagga ataactaatt ccctaatgaa actcgcgtcg cgccacttca
     1261 acgctcgatg ggagcgtcag cgtcgcgcca cttcaacgct cgatgggagc gtcattggtg
     1321 ggcggggtaa ccgtcgaaat cagtgtttag cccgggcagc atttttttca ctgcattcta
     1381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccca aactcatcaa
     1441 tgtatcttat catgtctgga tcgagtttca ttaaggaata actaattccc taatgaaact
     1501 cttggaatat aggacaatat cctaattgac ttatttaaat aatccattcc tgcaggtgcc
     1561 gtcctacgat ccgcagggat accagcagca gggctccggc ggctggcgcg cccttaagct
     1621 cgagggctcc ggcggcagcg gcttcgccaa cgagttgggc ccccgcttga tgggcaaggg
     1681 cggatctggc agtggctttg caaatgagtt aggtccgcgt ttgatgggta aaggcggctc
     1741 cgggagcggg ttcgcgaacg aattgggccc tcgcttaatg ggcaagggtg ggtcaggtag
     1801 tggatttgct aatgagttag gaccccgatt gatgggaaaa ggaggatctg gcagtggttt
     1861 cgccaacgaa ttggggccgc ggttaatggg gaaggggggt tcgggagtcg agggctccgg
     1921 cggcagcggc ttcgccaacg agttgggccc ccgcttgatg ggcaagggcg gatctggcag
     1981 tggctttgca aatgagttag gtccgcgttt gatgggtaaa ggcggctccg ggagcgggtt
     2041 cgcgaacgaa ttgggccctc gcttaatggg caagggtggg tcaggtagtg gatttgctaa
     2101 tgagttagga ccccgattga tgggaaaagg aggatctggc agtggtttcg ccaacgaatt
     2161 ggggccgcgg ttaatgggga aggggggttc gggagtcgag ggctccggcg gcagcggctt
     2221 cgccaacgag ttgggccccc gcttgatggg caagggcgga tctggcagtg gctttgcaaa
     2281 tgagttaggt ccgcgtttga tgggtaaagg cggctccggg agcgggttcg cgaacgaatt
     2341 gggccctcgc ttaatgggca agggtgggtc aggtagtgga tttgctaatg agttaggacc
     2401 ccgattgatg ggaaaaggag gatctggcag tggtttcgcc aacgaattgg ggccgcggtt
     2461 aatggggaag gggggttcgg gagtcgaggg ctccggcggc agcggcttcg ccaacgagtt
     2521 gggcccccgc ttgatgggca agggcggatc tggcagtggc tttgcaaatg agttaggtcc
     2581 gcgtttgatg ggtaaaggcg gctccgggag cgggttcgcg aacgaattgg gccctcgctt
     2641 aatgggcaag ggtgggtcag gtagtggatt tgctaatgag ttaggacccc gattgatggg
     2701 aaaaggagga tctggcagtg gtttcgccaa cgaattgggg ccgcggttaa tggggaaggg
     2761 gggttcggga gtcgagggct ccggcggcag cggcttcgcc aacgagttgg gcccccgctt
     2821 gatgggcaag ggcggatctg gcagtggctt tgcaaatgag ttaggtccgc gtttgatggg
     2881 taaaggcggc tccgggagcg ggttcgcgaa cgaattgggc cctcgcttaa tgggcaaggg
     2941 tgggtcaggt agtggatttg ctaatgagtt aggaccccga ttgatgggaa aaggaggatc
     3001 tggcagtggt ttcgccaacg aattggggcc gcggttaatg gggaaggggg gttcgggagt
     3061 cgagggctcc ggcggcagcg gcttcgccaa cgagttgggc ccccgcttga tgggcaaggg
     3121 cggatctggc agtggctttg caaatgagtt aggtccgcgt ttgatgggta aaggcggctc
     3181 cgggagcggg ttcgcgaacg aattgggccc tcgcttaatg ggcaagggtg ggtcaggtag
     3241 tggatttgct aatgagttag gaccccgatt gatgggaaaa ggaggatctg gcagtggttt
     3301 cgccaacgaa ttggggccgc ggttaatggg gaaggggggt tcgggagtcg agggctccgg
     3361 cggcagcggc ttcgccaacg agttgggccc ccgcttgatg ggcaagggcg gatctggcag
     3421 tggctttgca aatgagttag gtccgcgttt gatgggtaaa ggcggctccg ggagcgggtt
     3481 cgcgaacgaa ttgggccctc gcttaatggg caagggtggg tcaggtagtg gatttgctaa
     3541 tgagttagga ccccgattga tgggaaaagg aggatctggc agtggtttcg ccaacgaatt
     3601 ggggccgcgg ttaatgggga aggggggttc gggagtcgag ggctccggcg gcagcggctt
     3661 cgccaacgag ttgggccccc gcttgatggg caagggcgga tctggcagtg gctttgcaaa
     3721 tgagttaggt ccgcgtttga tgggtaaagg cggctccggg agcgggttcg cgaacgaatt
     3781 gggccctcgc ttaatgggca agggtgggtc aggtagtgga tttgctaatg agttaggacc
     3841 ccgattgatg ggaaaaggag gatctggcag tggtttcgcc aacgaattgg ggccgcggtt
     3901 aatggggaag gggggttcgg gagtcgaggg ctccggcggc agcggcttcg ccaacgagtt
     3961 gggcccccgc ttgatgggca agggcggatc tggcagtggc tttgcaaatg agttaggtcc
     4021 gcgtttgatg ggtaaaggcg gctccgggag cgggttcgcg aacgaattgg gccctcgctt
     4081 aatgggcaag ggtgggtcag gtagtggatt tgctaatgag ttaggacccc gattgatggg
     4141 aaaaggagga tctggcagtg gtttcgccaa cgaattgggg ccgcggttaa tggggaaggg
     4201 gggttcggga gtcgagggct ccggcggcag cggcttcgcc aacgagttgg gcccccgctt
     4261 gatgggcaag ggcggatctg gcagtggctt tgcaaatgag ttaggtccgc gtttgatggg
     4321 taaaggcggc tccgggagcg ggttcgcgaa cgaattgggc cctcgcttaa tgggcaaggg
     4381 tgggtcaggt agtggatttg ctaatgagtt aggaccccga ttgatgggaa aaggaggatc
     4441 tggcagtggt ttcgccaacg aattggggcc gcggttaatg gggaaggggg gttcgggagt
     4501 cgagggctcc ggcggcagcg gcttcgccaa cgagttgggc ccccgcttga tgggcaaggg
     4561 cggatctggc agtggctttg caaatgagtt aggtccgcgt ttgatgggta aaggcggctc
     4621 cgggagcggg ttcgcgaacg aattgggccc tcgcttaatg ggcaagggtg ggtcaggtag
     4681 tggatttgct aatgagttag gaccccgatt gatgggaaaa ggaggatctg gcagtggttt
     4741 cgccaacgaa ttggggccgc ggttaatggg gaaggggggt tcgggagtcg agggctccgg
     4801 cggcagcggc ttcgccaacg agttgggccc ccgcttgatg ggcaagggcg gatctggcag
     4861 tggctttgca aatgagttag gtccgcgttt gatgggtaaa ggcggctccg ggagcgggtt
     4921 cgcgaacgaa ttgggccctc gcttaatggg caagggtggg tcaggtagtg gatttgctaa
     4981 tgagttagga ccccgattga tgggaaaagg aggatctggc agtggtttcg ccaacgaatt
     5041 ggggccgcgg ttaatgggga aggggggttc gggagtcgag ggctccggcg gcagcggctt
     5101 cgccaacgag ttgggccccc gcttgatggg caagggcgga tctggcagtg gctttgcaaa
     5161 tgagttaggt ccgcgtttga tgggtaaagg cggctccggg agcgggttcg cgaacgaatt
     5221 gggccctcgc ttaatgggca agggtgggtc aggtagtgga tttgctaatg agttaggacc
     5281 ccgattgatg ggaaaaggag gatctggcag tggtttcgcc aacgaattgg ggccgcggtt
     5341 aatggggaag gggggttcgg gagtcgaggg ctccggcggc agcggcttcg ccaacgagtt
     5401 gggcccccgc ttgatgggca agggcggatc tggcagtggc tttgcaaatg agttaggtcc
     5461 gcgtttgatg ggtaaaggcg gctccgggag cgggttcgcg aacgaattgg gccctcgctt
     5521 aatgggcaag ggtgggtcag gtagtggatt tgctaatgag ttaggacccc gattgatggg
     5581 aaaaggagga tctggcagtg gtttcgccaa cgaattgggg ccgcggttaa tggggaaggg
     5641 gggttcggga gtcgagggct ccggcggcag cggcttcgcc aacgagttgg gcccccgctt
     5701 gatgggcaag ggcggatctg gcagtggctt tgcaaatgag ttaggtccgc gtttgatggg
     5761 taaaggcggc tccgggagcg ggttcgcgaa cgaattgggc cctcgcttaa tgggcaaggg
     5821 tgggtcaggt agtggatttg ctaatgagtt aggaccccga ttgatgggaa aaggaggatc
     5881 tggcagtggt ttcgccaacg aattggggcc gcggttaatg gggaaggggg gttcgggagt
     5941 cgagggctcc ggcggcagcg gcttcgccaa cgagttgggc ccccgcttga tgggcaaggg
     6001 cggatctggc agtggctttg caaatgagtt aggtccgcgt ttgatgggta aaggcggctc
     6061 cgggagcggg ttcgcgaacg aattgggccc tcgcttaatg ggcaagggtg ggtcaggtag
     6121 tggatttgct aatgagttag gaccccgatt gatgggaaaa ggaggatctg gcagtggttt
     6181 cgccaacgaa ttggggccgc ggttaatggg gaaggggggt tcgggagtcg acggatccgc
     6241 ggccgcataa tgggttcgcg ataggtcact gatatatatc atagctttta gttgtagtcg
     6301 gaaatcgttt gacgtttatt gtttgttctc ggtgtgccgt taaagttttc cccccttttt
     6361 ctctctattt gttgagttcc atcgatggac ggatccgtgg acctttcaga agtatataaa
     6421 tatataacta aagagtaacg aatttgtcag cgaataaggg gattccacat gccacactaa
     6481 cacaatgaaa ctatgtacct aacagagaaa ctacatatgc atacacagca ataccgataa
     6541 ccactaccaa taccattagc ccacatagac ccaaactggc ctaatcgatc tataaagcat
     6601 atatagtata tactattagg gtctcgctta cgcgaatttc tatttacaag ctccgaaatc
     6661 gaaactgcaa cacaagatag ggaaagaact ggaaatatat actcaacact tgtgtatatc
     6721 ataaatcaca taatccacac aaagcctatg ataattatat ttatatcggt ataatcgtat
     6781 atacatatat agccaaagat tgttgttgaa ctgcgaactg caagttttat taccgttgat
     6841 gatattatct tctgtgtaaa gagaacattt taatcaaacc caagcaattg ttgatgttcg
     6901 gcacgaatga taaccataaa aagaactagc taatcaaaag tcatatgaat actctgttaa
     6961 ctaattactg tttatgtttg ttgatattat ttaattcatt gatattaatg caccgtatgc
     7021 ttttgtgttt aactaacatg agcaaggcat gatgtaggta ctaaaaataa tacttaagta
     7081 gatagccgta gaagcttggc tcgatcgagc atggtcatag ctgtttcctg tgtgaaattg
     7141 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7201 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7261 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7321 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7381 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7441 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7501 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7561 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7621 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7681 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7741 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7801 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7861 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7921 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7981 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8041 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8101 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8161 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8221 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa
     8281 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     8341 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     8401 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     8461 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     8521 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     8581 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     8641 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     8701 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     8761 taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg catcatcagg
     8821 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     8881 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     8941 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     9001 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     9061 gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat
     9121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9241 tatcatgaca ttaacctata aaaataagcg tatcacgagg ccctttcgtc
//
