LOCUS       sequence-466704-        5816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enhancer near SATB2, chr2:199202570-199203402 cloned into pGL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5816)
  TITLE     Wysocka Lab enhancer plasmids 2025
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3_SATB2
FEATURES             Location/Qualifiers
     source          1..5816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..34)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(694..1485,1486..1554))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1317..1336
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1555..1659)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1686..2141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1773..1792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1983..2004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2272..2320
                     /note="synthetic polyadenylation signal"
     misc_feature    2334..2425
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2374..2393
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3280..3476
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3327..3462
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      3506..3515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3512..5164
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3578..3596)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5205..5326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5216..5235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5270..5289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5580..5597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(5751..5816,1..523))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
       61 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      361 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      601 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      661 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1681 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1741 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1981 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     2041 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     2101 atttaacgcg aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct
     2161 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     2221 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata
     2281 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     2341 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     2401 agtgcaggtg ccagaacatt tctctatcga taggtaccga gctcttacgc gtgctagctg
     2461 attttctcac cctatctcca cctctctaaa taacaatttt ccaaacctaa gactctgttt
     2521 tttgacaatt catccccatt gcaggcaccc atgtatgcac ggtcacacat tcgtatacac
     2581 acaaacacac agtttttctc ataacagatt tgcagagagg tcagaggcaa aggaggggga
     2641 aaacttaaaa aaaaaaaaaa aactccagta agtttgatct aaacaaaatg gttattcaaa
     2701 tgggtcccct actaatttta caaagatgcg tgataatatc caacatatgg ctttaactac
     2761 gttttttaat attagaaatt gaatgcacat tttaagacac aagttttgct gatatgctgt
     2821 tgataataca ggataactat tttccatttg tttctaatta tcttaaagac agcctgggga
     2881 tcatttaccc atggtttaag agcactcaga taagtccaca aatgcaacag gcgggcatgt
     2941 gtgtcagcta ctgcatcggg gctgcgtgag ggtggcttag ccaagactat cctcttatgt
     3001 ctgaaaccac tagatacggt tcctaaggtc aagcagtact aaaaaataaa atccttcctt
     3061 tgtcagcatt cttagggtgt gggctctgga gccagactgc ctggcaccac catttaaata
     3121 actgggtgac cttgtgcaag ttagttaacc tctctttgcc tcaatttcct tatctgaaaa
     3181 atggggctgt accaacaact tatggtcagc atgaggatga aagcatctag tatggtgccc
     3241 aaaagagaga aggcatgcaa taactcgaga tctgcgatct gcatctcaat tagtcagcaa
     3301 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3361 ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3421 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     3481 ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg
     3541 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     3601 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     3661 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     3721 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     3781 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     3841 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     3901 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     3961 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     4021 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     4081 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     4141 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     4201 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     4261 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     4321 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     4381 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     4441 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     4501 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     4561 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     4621 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     4681 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     4741 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     4801 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     4861 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
     4921 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     4981 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     5041 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     5101 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     5161 gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     5221 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5281 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5341 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5401 cctctacaaa tgtggtaaaa tcgataagga tctggatccg tcgaccgatg cccttgagag
     5461 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     5521 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     5581 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5641 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5701 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5761 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaa
//
