LOCUS       sequence-466709-        6506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  40X MYC tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6506)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-40XMYC
FEATURES             Location/Qualifiers
     source          1..6506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1384..1403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             1637..1666
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1679..1708
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1721..1750
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1763..1792
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1805..1834
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1865..1894
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1907..1936
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1949..1978
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1991..2020
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2033..2062
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2093..2122
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2135..2164
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2177..2206
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2219..2248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2261..2290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2321..2350
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2363..2392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2405..2434
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2447..2476
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2489..2518
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2549..2578
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2591..2620
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2633..2662
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2675..2704
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2717..2746
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2777..2806
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2819..2848
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2861..2890
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2903..2932
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2945..2974
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3005..3034
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3047..3076
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3089..3118
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3131..3160
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3173..3202
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3233..3262
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3275..3304
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3317..3346
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3359..3388
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3401..3430
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4330..4346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4330..4346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4343..4365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4354..4370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4378..4384,4385..4402,4403..4408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4423..4444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4561..4578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4732..5320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4812..4831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5491..6306)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5491..6306)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6214..6233
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(6307..6411)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaagtcat acatacacaa
      421 aaggacacgc acccacacag acccatcata gaaaccaatt tgaaattctg aatcctgctt
      481 gacgctcgtt aataaatgcc tggagcagtt tttaatgatt gctgagcttc tgtcactcat
      541 cccgtaaaat gtgtttccgg atttttgcag actgtgttca tgaactggac ggtcgccgtg
      601 gagagccatg tggactcgtc cttcttctgg ggctatctgg tgacgcagat tcccggcggc
      661 ttcatcgcct ccaagttccc ggccaacaag atattcggac tgtccatcgt gagctccgcc
      721 acgctgcatc tcttcgtgcc attcgccatg accctgatgc acggtcatgt ggtgatttgc
      781 gtgagggtcc tgcaaggact cttcgaggta tgtagcccaa ccaacctaac aacttgccca
      841 attcgatttt gccacagatc aaagccgggg attacggctg cgtgatgttg cgaaaatggg
      901 gagggttaga ttttgcttta tgataaagca aatatgtgaa atggaaacat gtcctaagcc
      961 ctcaaaggga tgggggtcct gggagggcag gaaaaaataa caaaagcatt tattttaatg
     1021 cgatttcttt gtgccaagga ctgggaagta tgtcattttt cttcgaatcg gaagggagag
     1081 aatgatactt tgtaaaaagg atgtttgtct attcgagttc cgaagtaact atattttaga
     1141 tatatatttt taatttcaca aagcacattt tcttaagata ctataactgc atgagactaa
     1201 gtgcagagtt tcattaagga ataactaatt ccctaatgaa actcgcgtcg cgccacttca
     1261 acgctcgatg ggagcgtcag cgtcgcgcca cttcaacgct cgatgggagc gtcattggtg
     1321 ggcggggtaa ccgtcgaaat cagtgtttag cccgggcagc atttttttca ctgcattcta
     1381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccca aactcatcaa
     1441 tgtatcttat catgtctgga tcgagtttca ttaaggaata actaattccc taatgaaact
     1501 cttggaatat aggacaatat cctaattgac ttatttaaat aatccattcc tgcaggtgcc
     1561 gtcctacgat ccgcagggat accagcagca gggctccggc ggctggcgcg cccttaagct
     1621 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     1681 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     1741 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     1801 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg agggctccgg
     1861 cggcgaacag aaacttatct cagaggagga tcttggctcc ggcggcgagc aaaaactaat
     1921 atcggaggaa gacctcggtt caggagggga acaaaagctc atttctgaag aggacctggg
     1981 atctgggggt gagcaaaagc tcatatcgga agaagacctc ggatctggtg gagaacagaa
     2041 actgatttcc gaagaggatc ttggttcggg gggggtcgag ggctccggcg gcgaacagaa
     2101 acttatctca gaggaggatc ttggctccgg cggcgagcaa aaactaatat cggaggaaga
     2161 cctcggttca ggaggggaac aaaagctcat ttctgaagag gacctgggat ctgggggtga
     2221 gcaaaagctc atatcggaag aagacctcgg atctggtgga gaacagaaac tgatttccga
     2281 agaggatctt ggttcggggg gggtcgaggg ctccggcggc gaacagaaac ttatctcaga
     2341 ggaggatctt ggctccggcg gcgagcaaaa actaatatcg gaggaagacc tcggttcagg
     2401 aggggaacaa aagctcattt ctgaagagga cctgggatct gggggtgagc aaaagctcat
     2461 atcggaagaa gacctcggat ctggtggaga acagaaactg atttccgaag aggatcttgg
     2521 ttcggggggg gtcgagggct ccggcggcga acagaaactt atctcagagg aggatcttgg
     2581 ctccggcggc gagcaaaaac taatatcgga ggaagacctc ggttcaggag gggaacaaaa
     2641 gctcatttct gaagaggacc tgggatctgg gggtgagcaa aagctcatat cggaagaaga
     2701 cctcggatct ggtggagaac agaaactgat ttccgaagag gatcttggtt cggggggggt
     2761 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     2821 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     2881 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     2941 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg agggctccgg
     3001 cggcgaacag aaacttatct cagaggagga tcttggctcc ggcggcgagc aaaaactaat
     3061 atcggaggaa gacctcggtt caggagggga acaaaagctc atttctgaag aggacctggg
     3121 atctgggggt gagcaaaagc tcatatcgga agaagacctc ggatctggtg gagaacagaa
     3181 actgatttcc gaagaggatc ttggttcggg gggggtcgag ggctccggcg gcgaacagaa
     3241 acttatctca gaggaggatc ttggctccgg cggcgagcaa aaactaatat cggaggaaga
     3301 cctcggttca ggaggggaac aaaagctcat ttctgaagag gacctgggat ctgggggtga
     3361 gcaaaagctc atatcggaag aagacctcgg atctggtgga gaacagaaac tgatttccga
     3421 agaggatctt ggttcggggg gggtcgacgg atccgcggcc gcataatggg ttcgcgatag
     3481 gtcactgata tatatcatag cttttagttg tagtcggaaa tcgtttgacg tttattgttt
     3541 gttctcggtg tgccgttaaa gttttccccc ctttttctct ctatttgttg agttccatcg
     3601 atggacggat ccgtggacct ttcagaagta tataaatata taactaaaga gtaacgaatt
     3661 tgtcagcgaa taaggggatt ccacatgcca cactaacaca atgaaactat gtacctaaca
     3721 gagaaactac atatgcatac acagcaatac cgataaccac taccaatacc attagcccac
     3781 atagacccaa actggcctaa tcgatctata aagcatatat agtatatact attagggtct
     3841 cgcttacgcg aatttctatt tacaagctcc gaaatcgaaa ctgcaacaca agatagggaa
     3901 agaactggaa atatatactc aacacttgtg tatatcataa atcacataat ccacacaaag
     3961 cctatgataa ttatatttat atcggtataa tcgtatatac atatatagcc aaagattgtt
     4021 gttgaactgc gaactgcaag ttttattacc gttgatgata ttatcttctg tgtaaagaga
     4081 acattttaat caaacccaag caattgttga tgttcggcac gaatgataac cataaaaaga
     4141 actagctaat caaaagtcat atgaatactc tgttaactaa ttactgttta tgtttgttga
     4201 tattatttaa ttcattgata ttaatgcacc gtatgctttt gtgtttaact aacatgagca
     4261 aggcatgatg taggtactaa aaataatact taagtagata gccgtagaag cttggctcga
     4321 tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4381 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4441 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4501 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4561 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4621 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4681 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4741 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4801 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4861 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4921 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4981 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5041 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5101 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5161 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5221 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5281 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5341 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5401 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5461 aagtatatat gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa
     5521 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     5581 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     5641 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     5701 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     5761 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     5821 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     5881 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     5941 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     6001 agggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     6061 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     6121 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     6181 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     6241 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     6301 gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6361 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6421 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6481 taagcgtatc acgaggccct ttcgtc
//
