LOCUS       sequence-466720-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 
            promoter with 2xMYC tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick 
            JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
  TITLE     Optimization of the fluorogen-activating protein tag for 
            quantitative protein trafficking and colocalization studies in S. 
            cerevisiae.
  JOURNAL   Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. 
            Epub 2024 May 29.
  PUBMED    38809589
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2024-07-1"; volume: "35(7):mr5. doi"; pages: " 
            10.1091/mbc.E24-04-0174. Epub 2024 May 29"
COMMENT     Sequence Label: pRS415-TEF1pr-SEC63-FAPoptim
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      681..1269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1170..1189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1423..1440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1557..1578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1593..1598,1599..1616,1617..1623)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1631..1647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1636..1658
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1655..1671
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1655..1671
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1690..1710
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1692..1710
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1728..2126
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     2130..2146
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2130..2146
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4509..4538
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4539..4568
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      4581..4828
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4597..4615)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4846..4865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4847..4865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4875..4892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4875..4891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4884..4906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5032..5487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5119..5138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5329..5350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5788..6195
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6196..7290
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(7784..7803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7903..7925
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7963..7981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8023..8526
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8563..8667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8668..8736,8737..9018,1..510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8886..8905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
       61 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      121 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      181 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      241 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      301 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      361 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      421 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
      481 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
      541 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      601 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     1621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     1681 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctcat agcttcaaaa
     1741 tgtttctact ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac
     1801 ttcaaaacac ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt
     1861 aattacccgt actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg
     1921 tcgaaaaagg caataaaaat ttttatcacg tttctttttc ttgaaaattt tttttttgat
     1981 ttttttctct ttcgatgacc tcccattgat atttaagtta ataaacggtc ttcaatttct
     2041 caagtttcag tttcattttt cttgttctat tacaactttt tttacttctt gctcattaga
     2101 aagaaagcat agcaatctaa tctaagtttt ctagaactag tggatccatg cctacaaatt
     2161 acgagtatga tgaggctagt gagacgtggc cgtccttcat tttaacgggg ctcttgatgg
     2221 tcgtcgggcc tatgacactg cttcaaatat accaaatttt ttttggggcc aatgctgaag
     2281 atgggaattc agggaagagt aaggagttta atgaggaagt tttcaagaac ttgaatgaag
     2341 aatacaccag tgatgaaatc aaacaattta gaaggaagtt tgataaaaat agtaataaga
     2401 agtccaaaat atggagcagg agaaatatta taattattgt gggttggatc ttagttgcaa
     2461 ttcttctgca aaggattaat agtaatgacg cgattaaaga cgctgctaca aaattatttg
     2521 atccttatga aatccttggt atctctacta gtgcttccga tagagacatc aaatctgctt
     2581 atagaaaatt atctgttaaa tttcatccag ataaattagc aaagggccta acacctgatg
     2641 agaaaagtgt gatggaagaa acttatgttc agattacgaa ggcttacgaa tcccttactg
     2701 acgaattggt taggcaaaac tatttgaaat acggtcatcc agatggccca caatctactt
     2761 cacatggtat cgctctacca agatttttgg tagatggaag tgcatctcca ttattagtgg
     2821 tttgttatgt tgcgctacta ggtttaatct tgccatattt tgttagtaga tggtgggcaa
     2881 gaacacaatc gtatactaag aagggaatac ataatgtgac ggcttctaat tttgttagta
     2941 acttagtcaa ttacaagcca tctgagattg tcaccacaga tttgatctta cactggttat
     3001 catttgctca tgaatttaaa caattcttcc cggatttgca accaacggat tttgaaaaac
     3061 ttttgcaaga tcatattaac cgcagagata gtggtaaact taacaatgcg aaatttagaa
     3121 tagtggccaa atgtcactct ttgttacacg gtttattgga tattgcttgt ggattcagaa
     3181 atttagatat tgcattgggt gcaatcaata ctttcaagtg tattgttcag gctgtaccat
     3241 taacaccaaa ctgtcaaatc cttcaattgc cgaacgtaga taaagagcac tttattacca
     3301 aaaccggaga tattcataca ttaggtaaat tgtttacttt agaagatgcc aagattggtg
     3361 aggttcttgg aataaaggat caagcaaagt taaacgaaac tttgagagtt gcatcgcata
     3421 ttccaaatct aaagatcatc aaggcagact tccttgtccc aggtgagaac caagtaacac
     3481 catcatctac cccatacatt tctttgaaag tactggttcg ttctgctaaa cagccattga
     3541 taccaactag cttaattcct gaagaaaatt taacagaacc tcaagatttt gaatctcaaa
     3601 gagatccatt tgctatgatg agtaaacagc cactcgtccc atattccttt gcaccatttt
     3661 tccctacaaa gagacgtggg agttggtgct gtctggtaag ttctcaaaaa gatggtaaaa
     3721 tacttcaaac gccaattatc attgaaaagc tatcttacaa gaacttgaac gatgacaaag
     3781 atttctttga taagaggata aaaatggatt taaccaaaca cgaaaaattc gatataaatg
     3841 attgggaaat cgggaccata aaaattccat taggtcagcc tgcacctgaa actgttggtg
     3901 atttcttttt tagagtaatc gttaaatcca cagattattt cactacagat ttggatatta
     3961 ccatgaatat gaaagttcgt gattctcctg cagtggaaca agtagaggtg tattctgagg
     4021 aggatgatga gtactctact gatgacgacg aaaccgaaag tgatgatgaa agtgatgcta
     4081 gcgattatac tgatatcgat acggatacag aagctgaaga tgatgaatca ccagaaaagc
     4141 ttatcgatac cgtcgacgcg ggtgcgggtg cgggtatgca agctgttgtt actcaagaac
     4201 catctgttac tgtttctcca ggtggtactg ttatcttgac ttgtggttct ggtactggtg
     4261 ctgttacttc tggtcactac gctaactggt tccaacaaaa gccaggtcaa gctccaagag
     4321 ctttgatctt cgacactgac aagaagtact cttggactcc aggtagattc tctggttctt
     4381 tgttgggtgc taaggctgct ttgactatct ctgacgctca accagaagac gaagctgaat
     4441 actactgttc tttgtctgac gttgacggtt acttgttcgg tggtggtact caattgactg
     4501 ttttgtctga gcagaagctg atcagcgagg aggacctgga gcagaagctg atcagcgagg
     4561 aggacctgta acgcctcgag tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     4621 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     4681 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     4741 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     4801 tttgggacgc tcgaaggctt taatttgcgg ccggtaccca attcgcccta tagtgagtcg
     4861 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4921 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4981 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     5041 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5101 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5161 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5221 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5281 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5341 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5401 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5461 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     5521 cggtatttca caccgcatat cgaccggtcg aggagaactt ctagtatatc tacataccta
     5581 atattattgc cttattaaaa atggaatccc aacaattaca tcaaaatcca cattctcttc
     5641 aaaatcaatt gtcctgtact tccttgttca tgtgtgttca aaaacgttat atttatagga
     5701 taattatact ctatttctca acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct
     5761 gattcaagaa atatcttgac cgcagttaac tgtgggaata ctcaggtatc gtaagatgca
     5821 agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg
     5881 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc
     5941 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg
     6001 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac
     6061 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg
     6121 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatatttcaa
     6181 ggatatacca ttctaatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt
     6241 ggtcaagaaa tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc
     6301 aatgtcaagt tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt
     6361 gttccacttc cagatgaggc gctggaagcc tccaagaagg ctgatgccgt tttgttaggt
     6421 gctgtgggtg gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa
     6481 atccgtaaag aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct
     6541 cttttagact tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc
     6601 agagaattag tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc
     6661 gcttgggata gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct
     6721 ttcatggccc tacaacatga gccaccattg cctatttggt ccttggataa agctaatgtt
     6781 ttggcctctt caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgaattccct
     6841 acattgaagg ttcaacatca attgattgat tctgccgcca tgatcctagt taagaaccca
     6901 acccacctaa atggtattat aatcaccagc aacatgtttg gtgatatcat ctccgatgaa
     6961 gcctccgtta tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca
     7021 gacaagaaca ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca
     7081 aagaataagg tcaaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca
     7141 ttgaacttgc ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca
     7201 ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc
     7261 gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac
     7321 ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt
     7381 tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag
     7441 gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa
     7501 aagaattgca ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt
     7561 gtaacagaaa atcatgaaac tatgattcct aatttatata ttggaggatt ttctctaaaa
     7621 aaaaaaaaat acaacaaata aaaaacactc aatgacctga ccatttgatg gagtttaagt
     7681 caataccttc ttgaaccatt tcccataatg gtgaaagttc cctcaagaat tttactctgt
     7741 cagaaacggc cttaacgacg tagtcgatat ggtgcactct cagtacaatc tgctctgatg
     7801 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7861 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7921 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7981 ttttataggt taatgtcatg ataataatgg tttcttagga cggatcgctt gcctgtaact
     8041 tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct gtgtttattt
     8101 atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa gagttacgga
     8161 atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt actttacata
     8221 tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa cgataagtaa
     8281 aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg aaacaattcg
     8341 gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg gcgatccccc
     8401 tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc tttttttact
     8461 ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat ttttatagca
     8521 cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8581 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8641 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8701 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8761 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8821 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8881 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8941 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     9001 acggatggca tgacagta
//
