LOCUS       sequence-466755-        9401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N EBNA3C
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1463..1489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1505..1529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1810..1819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4503..4527)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4540..5107
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4557..5109
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4701..4718)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4928..4947
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5121..5720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5121..5140)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5617..5637
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5796..6310
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6472..6488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6496..6502,6503..6520,6521..6526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6541..6562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6679..6696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6850..7438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6930..6949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7609..8400,8401..8469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8232..8251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8470..8574)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8642..8660
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8698..8720)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8820..8839
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9033..9055
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9183..9202
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctgcc accatggact acaaggatga
     1441 cgatgacaag ctcgatggag gatacccata cgatgttcca gattacgctg ctcgaggtta
     1501 tcaaacaagt ttgtacaaaa aagttggcat ggaatcattt gaagggcagg gggactctag
     1561 acagtcaccc gacaatgagc ggggagataa tgtacagact accggcgagc atgatcagga
     1621 ccctgggccg gggcctccat ccagtggggc ttctgagaga ttggtaccag aagagtcata
     1681 ctcaagagat cagcaacctt gggggcaaag caggggtgat gaaaacagag gctggatgca
     1741 gcgcatcagg cgaaggcgga gaagacgggc tgccttgtcc ggccatcttt tagacacgga
     1801 agacaatgtg ccgccatggt tgcctccaca cgacatcaca ccatataccg caaggaatat
     1861 cagggatgct gcctgccggg ctgtcaagca atcgcacctg caagcgctat caaacctgat
     1921 actcgatagt gggttagaca cacaacacat cttgtgcttc gtgatggcag ccaggcagcg
     1981 tcttcaggac attcgacgtg gacccttggt tgcggagggc ggtgtgggtt ggcgacattg
     2041 gcttctaaca tctcccagcc aatcctggcc catgggatat cgtacagcaa cactacgcac
     2101 attaactccc gtgcctaaca gggttggggc tgacagcatc atgttaactg ccacatttgg
     2161 atgccaaaat gcggcacgaa ctctaaacac cttctctgcc accgtgtgga caccacccca
     2221 tgctggacca agagagcaag aaagatacgc tcgggaagcc gaggtacgct tccttcgtgg
     2281 taaatggcag aggcggtacc gaagaatcta tgatttgata gaactgtgtg gctctctgca
     2341 ccacatctgg caaaacttgc tccagaccga ggagaacctt ttagatttcg tgcgtttcat
     2401 gggtgtcatg tccagctgca ataatccagc tgtgaattac tggtttcaca agacaatcgg
     2461 aaactttaag ccatattacc cgtggaatgc accacctaat gaaaatccat atcacgcgcg
     2521 gagaggcata aaagaacacg taatccagaa cgcatttcga aaggcacaaa tacagggttt
     2581 atcaatgtta gcaacgggag gtgaacccag aggtgatgct actagtgaaa cgagcagtga
     2641 tgaggacacc ggtagacagg gttcggacgt ggagctagag tcctcggacg atgagctgcc
     2701 atatatcgat cccaatatgg agccggttca gcagaggccc gtcatgtttg tgagccgtgt
     2761 gcctgcaaag aaaccgagga aactgccttg gcccacgccc aagacgcacc cagtgaagcg
     2821 cacaaatgtt aagacctctg atagatctga taaggcagaa gcacaaagca cccctgaaag
     2881 gccgggccct tccgaacaat catcagtgac cgtggagccc gcccacccga ccccggtgga
     2941 gatgccaatg gtgattctcc atcaaccacc tccagtgccc aaaccggttc cagtcaagcc
     3001 tacgccaccg ccttcccgta ggagaagggg agcgtgtgtt gtgtacgacg atgatgtcat
     3061 agaggtgatt gatgttgaaa ccaccgaaga ttcatcgtca gtgtcacagc caaataagcc
     3121 acatcggaaa catcaagacg gctttcaacg ttcaggccga cgtcaaaaac gagccgcgcc
     3181 tcccaccgtg agtccttcgg atactgggcc tcctgccgtg gggcctcctg ccgcggggcc
     3241 tcctgccgcg gggcctcctg ccgcggggcc tcctgccgcg gggcctcctg ccgcggggcc
     3301 tcctgccgcg gggcctcgca tactggcgcc tctttccgct gggcctcctg ccgcggggcc
     3361 tcacatagtg acgcctcctt ccgcccggcc tcgtataatg gcgcctcccg tcgtacgtat
     3421 gtttatgagg gagcgacagc tcccccagtc caccggccgt aaacctcagt gcttctggga
     3481 aatgcgggct ggtcgtgaaa ttacacaaat gcaacaagaa ccaagttcac acctgcagtc
     3541 cgccactcag cctacaacgc ctcgcccatc atgggcccca tcagtctgcg ccctctcggt
     3601 gatggatgct ggtaaggccc agcccataga aagttcacac ttgagttcca tgtcgcccac
     3661 acagccgata tcgcacgaag aacaaccccg gtatgaggat cctgacgctc ctctggattt
     3721 aagtttacat ccagacgttg ctgctcaacc agctccccag gctccatacc agggatacca
     3781 ggagccgccg gccccccagg ctccatacca gggataccag gagccgccgc ccccccaggc
     3841 tccataccag ggataccagg agccgccggc ccacgggctc caatcatctt catatccagg
     3901 atatgcgggt ccctggaccc caaggtctca acatccatgt tataggcacc cctgggcacc
     3961 atggtctcaa gatcctgtgc atgggcacac ccagggtcca tgggatccca gggcaccaca
     4021 tctcccacct cagtgggatg gatctgcagg acatggccag gatcaggtct cccagttccc
     4081 acatctgcaa tcggagacag gcccaccacg tcttcaactt tcattggtgc cactggtctc
     4141 atcctctgca ccatcatggt catctcccca gccccgagcc cccatacgcc ccattccaac
     4201 aagattcccc cctcccccta tgccgttaca agatagcatg gccgtggggt gtgactcatc
     4261 aggtacagca tgcccaagca tgccctttgc cagtgattac agtcaaggtg catttacccc
     4321 actggacatt aatgccacca cgccaaaaag gcctcgagta gaagaaagtt ctcacggacc
     4381 tgcccggtgt tcccaagcta ctgctgaagc acaggagatt ctcagtgaca attctgagat
     4441 ctccgtgttc ccaaaggacg cgaagcagac tgactacgat gcatccactg aaagtgagct
     4501 agatccaact ttcttgtaca aagtggttcg ataacgaatt ccgccccccc cccctaacgt
     4561 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4621 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4681 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4741 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4801 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4861 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4921 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4981 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5041 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     5101 atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5161 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5221 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5281 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5341 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5401 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5461 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5521 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5581 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5641 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5701 cccgcaagcc cggtgcctga tgcatcgatg tcgacctgca gccaagctta tcgataaaat
     5761 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     5821 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
     5881 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
     5941 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     6001 cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     6061 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     6121 tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg
     6181 atagactgcg tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     6241 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     6301 ggtctttcat gggtaacagt ttcttgaagt tggagaacaa cattctgagg gtaggagtcg
     6361 aatattaagt aatcctgact caattagcca ctgttttgaa tccacatact ccaatactcc
     6421 tgaaatagtt cattatggac agcgcagaaa gagctgggga gaattgtgaa attgttatcc
     6481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8641 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     8701 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8761 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     8821 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     8881 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     8941 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9001 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9061 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     9121 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     9181 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     9241 acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat atcagtggtc
     9301 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     9361 ataaaataaa agattttatt tagtctccag aaaaaggggg g
//
