LOCUS       sequence-46676-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Prince VE, Joly L, Ekker M, Ho RK
  TITLE     Zebrafish hox genes: genomic organization and modified colinear 
            expression patterns in the trunk.
  JOURNAL   Development. 1998 Feb . 125(3):407-20.
  PUBMED    9425136
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1998 Feb . 125(3):407-20."
COMMENT     Sequence Label: Hox B7a
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(650..666)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(650..666)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(703..721)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(797..815)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(798..815)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(833..849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(833..849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(846..868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    857..873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggccgcggga ttgttgcctt cagtattgta agtatcaact tttttattca gtgtatcccg
       61 aattaaagca agtcaaatca ataatatttt agtatagtct tattatgtct tcaacttgcg
      121 ttgctactca tcatcttctt cttcctcgtc gcctccaatt tgatcagcag caggcgagca
      181 gcggtccgtt gatttgttct ctttcttcca tttcattctc ctgttttgaa accaaatttt
      241 gatctggcgc tcggttaagc acagggcgtg tgctatctcg atacgccgcc gtcttgaaag
      301 gtaacggttg aagtgaaact ctttctccag ctctaatgtt tggtagcggg aataggtctg
      361 acggcctctt ttccggtcag cacccctgct gtacgtgtat tctctggctt gcactctccc
      421 gtgactctaa tggagctctg gcgttcagac ttccttctaa acatgaccaa agtcaacaac
      481 aggatctagt tagttcccgt acacataagc gctgaagtgt tcatctatcc atttagataa
      541 agaagccacc ttcgtaaaat atccatcttt ttagctgcag gtttaaagga aaaaaaatct
      601 aactagactt tcacaacctt gcctcgtgcc gaattcctgc agcccggggg atccactagt
      661 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
      721 tgaatcacta gtgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga
      781 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
      841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccgg
//
