LOCUS       sequence-466767-        7392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of replication/capsid proteins for AAV serotype 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Wehr Lab
  TITLE     TAOK2 controls synaptic plasticity and anxiety via ERK and calcium 
            signaling
  JOURNAL   iScience 2025, in press.
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 
            2025, in press."
COMMENT     Sequence Label: pAAV1_Rep52-VP1
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1821..1837)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1822..1838)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1862..1881)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1863..1881)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1891..1908)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1891..1907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1900..1922)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2049..2504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2136..2155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2346..2367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2530..2634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2635..2703,2704..3495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2853..2872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3666..4254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4155..4174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4408..4425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4542..4563
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4578..4583,4584..4601,4602..4608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4616..4632
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4621..4643
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4640..4656
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4640..4656
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4675..4695
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4677..4695
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5213..5224)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttctgatacg cctttttgac gacagaaacg ggttgagatt ctgacacggg aaagcactct
       61 aaacagtctt tctgtccgtg agtgaagcag atatttgaat tctgattcat tctctcgcat
      121 tgtctgcagg gaaacagcat cagattcatg cccacgtgac gagaacattt gttttggtac
      181 ctgtctgcgt agttgatcga agcttccgcg tctgacgtcg atggctgcgc aactgactcg
      241 cgcacccgtt tgggctcact tatatctgcg tcactggggg cgggtctttt cttggctcca
      301 ccctttttga cgtagaattc atgctccacc tcaaccacgt gatcctttgc ccaccggaaa
      361 aagtctttga cttcctgctt ggtgaccttc ccaaagtcat gatccagacg gcgggtgagt
      421 tcaaatttga acatccggtc ttgcaacggc tgctggtgtt cgaaggtcgt tgagttcccg
      481 tcaatcacgg cgcacatgtt ggtgttggag gtgacgatca cgggagtcgg gtctatctgg
      541 gccgaggact tgcatttctg gtccacgcgc accttgcttc ctccgagaat ggctttggcc
      601 gactccacga ccttggcggt catcttcccc tcctcccacc agatcaccat cttgtcgaca
      661 cagtcgttga agggaaagtt ctcattggtc cagtttacgc acccgtagaa gggcacagtg
      721 tgggctatgg cctccgcgat gttggtcttc ccggtagttg caggcccaaa cagccagatg
      781 gtgttcctct tgccgaactt tttcgtggcc catcccagaa agacggaagc cgcatattgg
      841 ggatcgtacc cgtttagttc caaaatttta taaatccgat tgctggaaat gtcctccacg
      901 ggctgctggc ccaccaggta gtcgggggcg gttttagtca ggctcataat ctttcccgca
      961 ttgtccaagg cagccttgat ttgggaccgc gagttggagg ccgcattgaa ggagatgtat
     1021 gaggcctggt cctcctggat ccactgcttc tccgaggtaa tccccttgtc cacgagccac
     1081 ccgaccagct ccatgtacct ggctgaagtt tttgatctga tcaccggcgc atcagaattg
     1141 ggattctgat tctctttgtt ctgctcctgc gtctgcgaca cgtgcgtcag atgctgcgcc
     1201 accaaccgtt tacgctccgt gagattcaaa caggcgctta aatactgttc catattagtc
     1261 cacgcccact ggagctcagg ctgggttttg gggagcaagt aattggggat gtagcactca
     1321 tccaccacct tgttcccgcc tccggcgcca tttctggtct ttgtgaccgc gaaccagttt
     1381 ggcaaagtcg gctcgatccc gcggtaaatt ctctgaatca gtttttcgcg aatctgactc
     1441 aggaaacgtc ccaaaaccat ggatttcacc ccggtggttt ccacgagcac gtgcatgtgg
     1501 aagtagctct ctcccttctc aaattgcaca aagaaaagag cctccggggc cttactcaca
     1561 cggcgccatt ccgtcagaaa gtcgcgctgc agcttctcgg ccacggtcag gggtgcctgc
     1621 tcaatcagat tcagatccat gtcagaatct ggcggcaact cccattcctt ctcggccacc
     1681 cagttcacaa agctgtcaga aatgccgggc agatgctcgt caaggtcgct ggggacctta
     1741 atcacaatct cgtaaaaccc cggcatggcg gctgcgcgtt caaacctccc gcttcaaaat
     1801 ggagaccctg cgagctcccc cgataccgtc gacctcgagg gggggcccgg tacccaattc
     1861 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     1921 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1981 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     2041 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2101 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2161 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2221 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2281 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2341 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2401 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2461 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     2521 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2581 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2641 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2701 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2761 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2821 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2881 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2941 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3001 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3061 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3121 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3181 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3241 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3301 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3361 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3421 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3481 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4321 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4381 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4441 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4501 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4561 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4621 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     4681 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagtcga
     4741 gcggccgcca gtgtgatgga tatctgcaga attcggcttg gcggctgcgc gttcaaacct
     4801 cccgcttcaa aatggagacc ctgcgtgctc actcgggctt aaatacccag cgtgaccaca
     4861 tggtgtcgca aaatgtcgca aaacactcac gtgacctcta atacaggacc tctagagcat
     4921 ggctacgatg acgtaagtct tttatcgcga agcgcaacca agcagttaat gtgtaagcta
     4981 taaccatggt aaccgataag ataagaagga caggagacca aagttcaact gaaacgaatc
     5041 aaccggttta ttgattaaca cgtaattaca ggggacgggt aaggtaacgg gtgccaatgg
     5101 ggcgaggctc agtataaagt ccattgttgt ccacagtaaa atcaacgttg gcagattttg
     5161 cataattgga tgtgtactgc acttcgggat tccagcgctt gctgttttct ttctgcagct
     5221 cccattcaat ttccacactc acttgtcctg tggagtattg ggtgatgaat gaagcaaact
     5281 ttgtagctga aaactccgcc ggaggattcg caggaacagg cgtgtttttg atgaggatct
     5341 gaggaggcgg gttcttgagt ccaaagccgc ccataagagg agacgggtga aagtgtccat
     5401 ctgtgtgagg aattttggcc caaatgggac cctgcaggta cacgtctcta tcttgccaca
     5461 ccatgccagg taatgctccc atagcatgca catctccggt cgcagggtct gtgctgctgc
     5521 tctggaaatt gactgccacg gtcccaaatc tttcggtggc cacagggtta gtggctttaa
     5581 tttcctcttc gtctgtaatc atgacactgt ccaatgcagt gtttgaagct ccggcgctct
     5641 cttttccaaa aatcatgaca ccgctcatgg gaaagaactt gtcttcgtcg tctttgtgtg
     5701 aggccatagc agtgccaggg ttgatgatgg attcacgccc attgaggtta tattttgaag
     5761 caccagtcca ggtaaaattg ctgttgttgt tgtctgtttt tgttttagaa acgcgctgct
     5821 gccgataaca gggtccaggt agccagtttt tgggctgaac agacatgcca gctggagacc
     5881 cacggctaaa cagcaagtcc ttgttttggg cacttccgga ctgattttga gttctgttca
     5941 ggtaatacag gtattggtcg atgagaggat tcatcagccg gtccaggctc tggctgtgcg
     6001 cgtagctgct gtggaaaggc acttcctcaa aggtgtagct gaaggtaaag ttgttgcccg
     6061 ttctcagcat ctgagaaggg aaatattcca ggcagtaaaa ggatgaacgt cccacggctt
     6121 ggctgccatt gttgagcgtc aggtagccgt attgcggaat catgaacacg tccgccggga
     6181 acggagggag gcagccctgg tgcgcagagc cgaggacgta cggaagctgg tactccgagt
     6241 ccgagaagac ttgaaccgtg ctggtaaggt tattagcgat ggttgtgacg ccatcattcg
     6301 tcgtgacctc cttgacttgg atgttgaaga gtttgaagtt gagtctcttg ggccggaatc
     6361 cccaattgtt gttgatgagt cgctgccagt cacgtggtga aaagtggcag tggaatctgt
     6421 tgaaatcaaa atacccccag ggggtgctgt agccgaagta gtggttgtcg ttgctggccc
     6481 ccgttgaagc actggagatt tgcttgtaga ggtggttatt gtaggtgggc aaggcccagg
     6541 tgcgggtgct ggtggtgatg actctgtcgc ccagccatgt ggaatcgcaa tgccaatttc
     6601 ctgaggcatt acccactccg tcggcgcctt cgttattgtc tgccattggt gcgccaccgc
     6661 ctgaagccat tgtagtaggt cccacagcag cgggggttgc tggaggttct ccgagaggtt
     6721 gtggatcggg gactgactct gagtcgccag tctgaccaaa attgagtctc tttttagcgg
     6781 gctgctggcc tgtcttgccg atgcccgagg aggagtctgg ctcttgtggc gactgctcta
     6841 ccggacgttt ctttccagga gccgtcttag cgccttcctc aaccagaccg agaggttcga
     6901 gaacccgctt cttggcctgg aagactgctc gcccgaggtt gcccccaaaa gacgtatctt
     6961 cttgcagacg ctcctgaaac tcggcgtcgg cgtggttata ccgcaggtac ggattgtcac
     7021 ccgctttgag ctgctggtcg taggccttgt cgtgctcgag ggccgctgcg tccgccgcgt
     7081 tgacgggctc ccccttgtcg agtccgttga agggtccgag gtacttgtag ccaggaagca
     7141 ccagaccccg gccgtcgtcc tgcttttgct ggttggcttt gggcttcggg gctccaggtt
     7201 tcaagtccca ccactcgcga atgccctcag agaggttgtc ctcgagccaa tctggaagat
     7261 aaccatcggc agccatacct gatttaaatc atttattgtt caaagatgca gtcatccaaa
     7321 tccacattga ccagatcgca ggcagtgcaa gcgtctggca cctttcccat gatatgatga
     7381 atgtagcaca gt
//
