LOCUS       sequence-46678-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pMartini Gate A R1-R2
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..182
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(223..528)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(325..344)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgccagcggc cgccggcctc gagggccgga tccgaattcc cgggagagct cgatatcacc
       61 actttgtaca agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      121 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      181 tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat
      241 caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
      301 gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
      361 gatatgcacc accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc
      421 cagggggatc accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa
      481 cagacgataa cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc
      541 tgttctcgtc agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt
      601 cctgattttc cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg
      661 tgcttaccag accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct
      721 gtcaactgtc actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta
      781 tacatatcag tatatattct tataccgcaa aaatcagcgc gcaaatacgc atactgttat
      841 ctggctttta gtaagccgga tccacgcgtt tacgccccgc cctgccactc atcgcagtac
      901 tgttgtaatt cattaagcat tctg
//
