LOCUS       sequence-466782-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate HA-tagged human MYADM stable 
            expressing cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Qiao W, Richards CM, Kim Y, Zengel JR, Ding S, Greenberg HB, Carette
            JE
  TITLE     MYADM binds human parechovirus 1 and is essential for viral entry.
  JOURNAL   Nat Commun. 2024 Apr 24;15(1):3469. doi: 10.1038/s41467-024-47825-0.
  PUBMED    38658526
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-24"; volume: "15(1):3469. doi"; pages: " 
            10.1038/s41467-024-47825-0"
COMMENT     Sequence Label: plenti-MYADM-HA
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2998..3301
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3302..3505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3456..3476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3502..3526
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4575..4601
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4631..5219
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4684..4704)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5102..5113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5233..5732
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5268..5291)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5665..5684
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5753..6352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5753..5772)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6249..6269
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6490..6723
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6795..6916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6832..6851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6886..6905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6956..7091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7111..7130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7112..7130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7140..7157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7140..7156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7149..7171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7298..7753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7385..7404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7595..7616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7779..7883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7884..7952,7953..8744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8102..8121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     3001 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3061 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3121 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3181 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3241 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3301 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3361 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3421 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3481 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     3541 tccacgctgt tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg
     3601 cagataccat gcccgtgacg gtcacacgca ctacaattac cacaacgacc acaagtagtt
     3661 ctggcttggg atctccgatg attgtcggtt ctcccagggc cctcacccaa cctttgggcc
     3721 ttctgagact tctccagctc gtgtctacct gcgtcgcctt ctcccttgta gcctctgtgg
     3781 gggcttggac tggctcaatg ggaaattgga gcatgtttac gtggtgcttt tgtttcagcg
     3841 ttacccttat catactgata gtggaactct gcgggctgca agctcgcttc ccattgtcat
     3901 ggcgaaactt ccccattact ttcgcttgct atgccgccct cttctgcctc tcagcgagta
     3961 taatataccc taccacttat gtgcagttcc tgtctcacgg acgatcacga gatcacgcta
     4021 tagcagcgac gtttttctct tgtatcgcct gcgttgcata tgccacagag gtggcctgga
     4081 cgagggcgag accgggagaa ataacagggt atatggcaac tgtgccgggc ttgcttaaag
     4141 tgcttgagac atttgttgcg tgcatcattt tcgcatttat ttccgacccg aatctttacc
     4201 agcatcagcc tgctttggaa tggtgtgtgg ccgtatacgc tatttgcttc attctcgctg
     4261 caattgcaat actccttaat cttggagaat gcacaaatgt tttgcctatt ccatttccct
     4321 cttttttgag cggattggct ctcctgtccg tgctcctcta cgcaacggcc ctcgtgttgt
     4381 ggccccttta tcagtttgac gaaaaatacg gagggcaacc ccgacgatca cgggacgtat
     4441 cttgttcaag atcacatgcc tactacgttt gcgcctggga ccggcggctt gcggtggcaa
     4501 ttttgactgc cataaacttg ctcgcatatg tggcagacct ggtgcactct gcgcacctcg
     4561 tttttgttaa ggtatatccg tatgatgttc cggattatgc ataaatccag cacagtggcg
     4621 gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4681 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4741 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4801 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4861 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4921 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4981 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     5041 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5101 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5161 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5221 tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     5281 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     5341 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5401 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5461 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5521 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     5581 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     5641 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     5701 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     5761 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5821 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5881 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5941 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6001 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6061 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6121 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6181 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6241 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6301 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     6361 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg
     6421 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6481 aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta
     6541 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6721 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6781 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6901 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7081 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7141 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7201 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7261 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7741 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7801 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7861 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7921 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7981 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8041 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8101 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8161 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8221 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8281 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8341 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8401 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8461 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8521 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8581 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8641 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8701 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8761 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8821 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8881 cgtcagaccc cgtagaaaag atcaaaggat cttc
//
