LOCUS       sequence-466825-        4056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Tau-RING protein in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Miller LVC, Papa G, Vaysburd M, Cheng S, Sweeney PW, Smith A, Franco
            C, Katsinelos T, Huang M, Sanford SAI, Benn J, Farnsworth J, 
            Higginson K, Joyner H, McEwan WA, James LC
  TITLE     Co-opting templated aggregation to degrade pathogenic tau assemblies
            and improve motor function.
  JOURNAL   Cell. 2024 Oct 17;187(21):5967-5980.e17. doi: 
            10.1016/j.cell.2024.08.024. Epub 2024 Sep 13.
  PUBMED    39276772
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.08.024"; journalName: "Cell"; date: 
            "2024-10-17- 17"; volume: "187"; issue: "21"; pages: "5967-5980.e17"
COMMENT     Sequence Label: pOPTG-Tau-RING
FEATURES             Location/Qualifiers
     source          1..4056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(96..115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      909..1497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1398..1417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1651..1668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1683..1822
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1748..1767)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1908..1930
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1983..2002
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1983..2001
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2075..2097
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2111..2128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3637..3655)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3651..3698
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(3743..3761)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3829..3933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3934..4002,4003..4056,1..738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1681 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
     1741 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     1801 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     1861 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1921 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggatctcg atcccgcgaa
     1981 attaatacga ctcactatag ggagaccaca acggtttccc tctagtgccg gctccggaga
     2041 gctccaattg gaattcgcta gctctagaaa taattttgtt taactttaag aaggagatat
     2101 aaatatggct catcatcacc atcaccatat gaccatggca gaaccgcgtc aagaatttga
     2161 agttatggaa gatcatgcag gcacctatgg tctgggtgat cgtaaagatc aaggtggtta
     2221 taccatgcat caggatcaag agggtgatac cgatgcaggt ctgaaagccg aagaagcagg
     2281 tattggcgat accccgagcc tggaagatga agcagcaggt catgttaccc aggctcgtat
     2341 ggttagcaaa agcaaagatg gcaccggtag tgatgataaa aaggcaaaag gtgcagatgg
     2401 caaaaccaaa attgcaacac cgcgtggtgc agcaccgcct ggtcagaaag gtcaggcaaa
     2461 tgcaacccgt attccggcaa aaacccctcc ggcaccgaaa acacctccga gcagcggtga
     2521 accgcctaaa agcggtgatc gttcaggtta tagcagtccg ggttctccgg gtacaccggg
     2581 tagccgtagc cgtacaccga gcctgccgac accgcctaca cgtgaaccga aaaaggttgc
     2641 agttgttcgt acccctccga aaagcccgag cagtgcaaaa agtcgcctgc agaccgcacc
     2701 ggttccgatg cctgatctga aaaatgttaa aagcaaaatc ggcagcaccg agaacctgaa
     2761 acatcagcct ggtggtggta aagttcagat cattaataag aaactggacc tgagcaatgt
     2821 gcagagcaaa tgtggtagca aagataacat taaacatgtg agcggtggtg gcagcgttca
     2881 gattgtgtat aaaccggttg atctgagcaa agttaccagc aaatgcggtt cactgggtaa
     2941 tattcatcat aaaccaggtg gtggtcaggt tgaagtgaaa agcgaaaaac tggatttcaa
     3001 agatcgcgtc cagagcaaaa ttggctcact ggataacatc acccatgttc caggtggcgg
     3061 taacaaaaag attgaaaccc ataaactgac cttccgcgaa aatgcaaaag ccaaaaccga
     3121 tcatggtgcc gaaatcgtgt ataaaagtcc ggttgttagc ggtgatacca gtccgcgtca
     3181 tctgagtaat gttagcagta ccggtagcat tgatatggtt gatagtccgc agctggcaac
     3241 cctggccgat gaagttagcg caagcctggc aaaacaaggt ctggaatttg gtagcggtgg
     3301 cggttcaggt atggcaagcg cagcacgtct gaccatgatg tgggaagaag ttacctgtcc
     3361 gatttgtctg gacccgtttg ttgaaccggt tagtattgaa tgtggtcaca gcttttgtca
     3421 agaatgcatt agccaggttg gtaaaggcgg tggtagcgtt tgtccggttt gtcgtcagcg
     3481 ttttctgctg aaaaatctgc gtccgaatcg tcagctggcc aatatggtta ataatctgaa
     3541 agaaatcagc caagaggcac gcgaataagg taccacgcgt gcgcgctgat ccggctgcta
     3601 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     3661 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatagccg
     3721 gataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3781 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3841 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4021 aaagtaaaag atgctgaaga tcagttgggt gcacga
//
