LOCUS       sequence-466891-       11243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid contains human HCFC1 full length gene with HA and c-myc 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Wilson AC, LaMarco K, Peterson MG, Herr W
  TITLE     The VP16 accessory protein HCF is a family of polypeptides processed
            from a large precursor protein.
  JOURNAL   Cell. 1993 Jul 16;74(1):115-25.
  PUBMED    8392914
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1993-07-16"; volume: "74(1)"; pages: "115-25"
COMMENT     Sequence Label: pCGN-HCF-1 fl
FEATURES             Location/Qualifiers
     source          1..11243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3128..3145
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          6941..7513
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(7645..7664)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7710..7765
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7764..7783)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      8119..8254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8360..8377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8531..9119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8611..8630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9290..10081,10082..10150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9913..9932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10151..10255)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10323..10341
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10379..10401)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10501..10520
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(10587..11042)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10724..10745)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10936..10955
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11168..11190
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11182..11199
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11183..11199
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11206..11225
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11206..11224
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(11243,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agatcacgtc
      721 gtgcatctgg tacgtacgga cgcgtcgctt cggccgtgtc gcccgccaac ttgccagcgg
      781 tgcttctgca gccccgctgg aagcgagtgg tgggctggtc gggtccggtg ccacggcccc
      841 gccacggcca ccgcgccgtg gccatcaagg agctcatcgt ggtgtttggc ggcggcaacg
      901 agggaatagt ggacgaactg cacgtgtaca acacggcaac caaccagtgg ttcatcccag
      961 ccgtgagggg ggacattccc cctgggtgtg cagcctatgg cttcgtgtgt gacgggactc
     1021 gcctcctggt gtttggtggg atggtggagt atgggaaata cagcaatgac ctctacgaac
     1081 tccaggcgag ccggtgggag tggaagagac tcaaagcaaa gacgcccaaa aacgggcccc
     1141 ctccgtgtcc tcgactcggg cacagcttct cccttgtggg caacaaatgc tacctgtttg
     1201 ggggtctggc caatgatagc gaggacccaa agaacaacat tccaaggtac ctgaatgact
     1261 tatatatcct ggaattacgg ccaggctctg gagtggtagc ctgggacatt cccatcactt
     1321 acggggtcct accaccaccc cgggagtcac atactgccgt ggtctacacc gaaaaagaca
     1381 ataagaagtc caagctggtg atctacggcg ggatgagtgg ctgcaggctg ggggacctgt
     1441 ggaccctaga tattgacacc ctgacgtgga ataagcccag tctcagcggg gtggcgcctc
     1501 ttcctcgcag tctccactcg gcaaccacca tcggaaataa aatgtacgtg tttggtggct
     1561 gggtgcctct cgtcatggat gacgtcaaag tggccacaca cgagaaggag tggaagtgta
     1621 ccaacacgct ggcttgtctc aacctggata ccatggcctg ggagaccatc ctgatggata
     1681 cactggagga caacatcccc cgtgctcggg ctggccactg cgcagtcgcc atcaacaccc
     1741 gcctgtacat ttggagtggg cgtgacggct accgcaaggc ctggaacaac caggtctgct
     1801 gcaaggacct ctggtaccta gagacagaaa agccaccacc cccagcccga gtacaactgg
     1861 tacgcgccaa caccaactcc ctggaggtga gctggggggc agtggcaaca gccgacagct
     1921 accttctcca gctccagaaa tatgacattc ctgccacggc tgctactgcc acctccccta
     1981 cacccaatcc ggtcccatct gtgcctgcca accctcccaa gagccctgcc ccagcagcag
     2041 ccgcacctgc tgtgcagccg ctgacccaag taggcatcac gctcctgccc caggctgccc
     2101 ccgcaccccc gaccaccacc accatccagg tcttgccaac ggtgcctggc agctccattt
     2161 ctgtgcccac cgcagccagg actcaaggtg tccctgctgt tctcaaagtg accggtcctc
     2221 aggctacaac aggaactcca ttggtcacca tgcgacctgc cagccaggct gggaaagccc
     2281 ctgtcaccgt gacctccctt cccgccggag tgcggatggt tgtgccaaca cagagtgccc
     2341 agggaacggt gattggcagt agcccacaga tgagtgggat ggccgcactg gccgctgcgg
     2401 ccgctgccac ccagaagatc cccccttcct cggcacccac ggtgctgagt gtcccagcgg
     2461 gtaccaccat cgtgaagacc atggctgtga cacctggcac taccaccctc ccagccactg
     2521 tgaaggtggc ctcctcgcca gtcatggtga gcaaccctgc cactcgcatg ctgaagactg
     2581 cagccgccca ggtggggaca tcggtttcct ccgccaccaa cacgtctacc cgccctatca
     2641 tcacagtgca caagtcaggc actgtgacag tggcccagca agcccaggtg gtgaccacag
     2701 ttgtgggcgg ggtcaccaag accatcaccc tggtgaagag ccccatctct gtcccaggag
     2761 gcagtgctct gatttccaat ctgggcaaag tgatgtcggt ggtccagacc aaaccagttc
     2821 agacttcagc agtcacaggc caggcgtcca cgggtcctgt gactcagatc atccagacca
     2881 aagggcccct gccagcggga acaatcctga agctggtgac ctcagcagat ggcaagccca
     2941 ccaccatcat cactaccacg caggccagtg gggcggggac caagcccacc atcctgggca
     3001 tcagcagcgt ctcccccagt accaccaagc ccggcacgac caccatcatc aaaaccatcc
     3061 ccatgtcggc catcatcacc caggcgggcg ccacgggtgt gaccagcagt cctggcatca
     3121 agtcccccat caccatcatc accaccaagg tgatgacttc aggaactgga gcacctgcga
     3181 aaatcatcac tgctgtcccc aaaattgcca ctggccacgg gcagcaggga gtgacccagg
     3241 tggtgcttaa gggggccccg ggacagccag gcaccatcct ccgcactgtg cccatggggg
     3301 gtgttcgcct ggtcacaccc gtcaccgtct ccgccgtcaa gccagccgtc accacgttgg
     3361 ttgtgaaagg caccacaggt gtcacgaccc taggcacagt gacaggcacc gtctccacca
     3421 gccttgccgg ggcggggggc cacagcacta gtgcttccct ggccacgccc atcaccacct
     3481 tgggcaccat tgccaccctc tcaagccagg tgatcaaccc cactgccatc actgtgtcgg
     3541 ccgcacagac cacgctgaca gcggcaggcg ggctcacaac cccaaccatc accatgcagc
     3601 ccgtgtccca gcccacccag gtaactctga tcacggcacc tagtggggtg gaggcccagc
     3661 ctgtgcatga cctccctgtg tccattctgg cctccccgac tacagaacag cccaccgcca
     3721 cagttaccat cgccgactca ggccagggtg atgtgcagcc tggcactgtc accttggtgt
     3781 gctccaaccc accctgtgag acccacgaga ctggcaccac caacacggcc accactactg
     3841 ttgtggctaa ccttggggga cacccccagc ccacccaagt gcagttcgtc tgtgacagac
     3901 aggaggcagc tgcttctctt gtgacctcga ctgtgggcca gcagaatggt agcgtggtcc
     3961 gagtctgttc gaacccgccc tgcgagaccc acgagacggg caccaccaac accgccacca
     4021 ccgccacctc caacatggcc gggcagcatg gctgctcaaa cccaccctgc gagacccacg
     4081 agacgggcac caccaacact gccactacag ccatgtcgag cgtcggcgcc aaccaccagc
     4141 gagatgcccg tcgggcctgt gcagctggca cccctgccgt gatccggatc agtgtggcca
     4201 ctggggcgct ggaggcagcc cagggctcta agtcccagtg ccaaacccgc cagaccagcg
     4261 cgaccagcac caccatgact gtgatggcca ccggggcccc gtgctcggcc ggcccactcc
     4321 ttgggccgag catggcacgg gagcccgggg gccgcagccc tgcttttgtg cagttggccc
     4381 ctctgagcag caaagtcagg ctgagcagcc caagcattaa ggaccttcct gcggggcgcc
     4441 acagccatgc ggtcagcacc gctgccatga cccgttccag cgtgggtgct ggggagcccc
     4501 gcatggcacc tgtgtgcgag agcctccagg gtggctcgcc cagcaccaca gtgactgtga
     4561 cagccctgga ggcactgctg tgcccctcgg ccaccgtgac ccaagtctgc tccaacccac
     4621 catgtgagac ccacgagaca ggcaccacca acaccgccac tacctcgaat gcaggcagcg
     4681 cccagagggt gtgctccaac ccgccatgcg agacccacga gacgggcacc acccacacgg
     4741 ccaccaccgc tacttcaaac gggggcacgg gccagcccga gggtgggcag cagccccctg
     4801 ctggtcgccc ctgtgagaca caccagacca cttccactgg caccaccatg tcggtcagcg
     4861 tgggtgccct gcttcccgac gccacttctt cccacaggac cgtggagtct ggcctagagg
     4921 tggcggcggc acccagcgtc accccccagg ctggcaccgc gctgctggct cctttcccaa
     4981 cacagagggt gtgctccaac cccccctgtg agacccacga gacgggcacc actcacacgg
     5041 ccaccactgt cacttccaac atgagttcaa accaagaccc cccacctgct gccagcgatc
     5101 agggagaggt ggagagcacc cagggcgaca gcgtgaacat caccagctcc agtgccatca
     5161 cgacaaccgt gtcctccaca ctgacgcggg ctgtgaccac cgtgacgcag tccacaccgg
     5221 tcccgggccc ctctgtgccg cccccagagg aactccaggt gtcgccaggt cctcgccagc
     5281 agctgccgcc acggcagctt ctgcagtcgg cttccacagc cctgatgggg gagtccgccg
     5341 aggtcctgtc agcctcccag acccctgagc tcccggccgc cgtggatctg agcagcacag
     5401 gggagccatc ttcgggccag gagtctgccg gctctgcggt ggtggccact gtggtggtcc
     5461 agccaccccc acccacacag tccgaagtag accagttatc acttccccaa gagctaatgg
     5521 ccgaggccca agctggcacc accaccctca tggtaacggg gctcaccccc gaggagctgg
     5581 cagtgacggc tgctgcagaa gcagctgccc aggccgcagc cacggaggaa gcccaggccc
     5641 tggccatcca ggcggtgctc caggccgcgc agcaggccgt catgggcacc ggcgagccca
     5701 tggacacctc cgaggcagca gcaaccgtga ctcaggcgga gctggggcac ctgtcggccg
     5761 agggtcagga gggccaggcc accaccatac ccattgtgct gacacagcag gagctggctg
     5821 ccctggtgca gcagcagcag ctgcaggagg cccaggccca gcagcagcat caccacctcc
     5881 ccactgaggc cctggcccct gccgacagtc tcaacgaccc agccattgag agcaattgcc
     5941 tcaatgagct ggccggcacg gtccccagca ctgtggcgct gctgccctca acggccactg
     6001 agagcctggc tccatccaac acatttgtgg ccccccagcc ggttgtggtg gccagcccag
     6061 ccaagctgca ggctgcagct accctgaccg aagtggccaa tggcatcgag tccctgggtg
     6121 tgaagccaga cctcccgccc ccacccagca aagcccccat gaagaaggaa aaccagtggt
     6181 ttgatgtggg agtcattaag ggcaccaatg taatggtgac acactatttc ctgccaccag
     6241 atgatgctgt cccatcagac gatgatttgg gcaccgtccc tgactataac cagctgaaga
     6301 agcaggagct gcagccaggc acagcctata agtttcgtgt tgccggaatc aatgcctgtg
     6361 gccgggggcc cttcagcgaa atctcagcct ttaagacgtg cctgcctggt ttcccagggg
     6421 ccccttgtgc cattaaaatc agcaaaagtc cggatggtgc tcacctcacc tgggagccac
     6481 cctctgtgac ctccggcaag attatcgagt actccgtgta cctggccatc cagagctcac
     6541 aggctggggg cgagctcaag agctccaccc cggcccagct ggccttcatg cgggtgtact
     6601 gtgggcccag cccctcctgc ctggtgcagt cctccagcct ttccaacgcc cacatcgact
     6661 acaccaccaa gcccgccatc atcttccgca tcgccgcccg caatgagaag ggctatggcc
     6721 cggccacaca agtgaggtgg ctgcaggaaa ccagtaaaga cagctctggc accaagccag
     6781 ccaacaagcg gcccatgtcc tctccagaaa tgaaatctgc tccaaagaaa tctaaggccg
     6841 atggtcaggg cgagcagaag ctgatctctg aggaggacac ggtatcgcga taagcttgat
     6901 atcgaattcc tgcagcccgg gggatcctga gaacttcagg gtgagtttgg ggacccttga
     6961 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     7021 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     7081 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     7141 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     7201 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     7261 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     7321 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     7381 caccctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     7441 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     7501 ctctttccta cagctcctgg gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa
     7561 agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     7621 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     7681 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     7741 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     7801 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     7861 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     7921 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     7981 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     8041 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgagga
     8101 gctttttgca aaagcctagg cctccaaaaa agcctcttca ctacttctgg aatagctcag
     8161 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     8221 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     8281 ttgctgacta attgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8341 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8401 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8461 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8521 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8581 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8641 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8701 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8761 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8821 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8881 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8941 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9001 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9061 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9121 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9181 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9241 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9301 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9361 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9421 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9481 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9541 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9601 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9661 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9721 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9781 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9841 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9901 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9961 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10021 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10081 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10141 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10201 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10261 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10321 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10381 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10441 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10501 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10561 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
    10621 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
    10681 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
    10741 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
    10801 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
    10861 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
    10921 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
    10981 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
    11041 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    11101 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    11161 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
    11221 tagggcgaat tcgagctcgc ccc
//
