LOCUS       sequence-466913-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2185
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(119..187,188..979)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(337..356)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1150..1738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2026..2047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2062..2067,2068..2085,2086..2092)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2100..2116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2124..2140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2161..2179
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2971..2992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3007..3012,3013..3030,3031..3037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3045..3061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3069..3085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3106..3124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3152..3378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3379..3559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3606..3731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4224..4457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4642..4686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4917..5127
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4939..5135
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4986..5121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5167..5769
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5167..5186)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5663..5683
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5812..5929
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6067..7245
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6298..7236
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7193..7213
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7256..7265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8308..8896
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8361..8381)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8779..8790)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8968..9201
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9279..9400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9316..9335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9370..9389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9440..9575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9595..9614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9596..9614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9624..9641)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9624..9640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9633..9655)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9782..10237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9869..9888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10079..10100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
       61 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      121 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      181 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      241 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      301 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      361 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      421 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      481 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      541 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      601 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      661 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      721 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      781 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
      841 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      901 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      961 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1021 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1081 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1261 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1741 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1801 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1861 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1921 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1981 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2041 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     2101 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     2161 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     2221 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     2281 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     2341 aggcaacaga cgggtctgac atggattgga ctcaagacga tagttaccgg ataaggcgca
     2401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3001 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     3121 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     3181 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     3241 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     3301 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     3361 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3421 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3481 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3541 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     3601 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     3661 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     3721 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     3781 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     3841 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     3901 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     3961 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4021 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4081 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4141 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     4201 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     4261 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     4321 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     4381 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     4441 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     4501 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4561 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     4621 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     4681 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     4741 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     4801 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     4861 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4921 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
     4981 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5041 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5101 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
     5161 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5221 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5281 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5341 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     5401 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5461 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5521 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5581 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5641 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5701 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5761 gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     5821 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     5881 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     5941 cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata
     6001 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     6061 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6121 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     6181 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6241 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     6301 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     6361 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     6421 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     6481 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     6541 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     6601 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     6661 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     6721 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     6781 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     6841 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     6901 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     6961 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     7021 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     7081 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     7141 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     7201 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcaccgccac
     7261 catggaggag aaaccctcag ggcccatccc agacatgctg gccactgcag agcccagctc
     7321 cagtgagacc gacaaggagg tgttgtcccc ggctgtgcca gctgcagccc cttcctcctc
     7381 catgtcggag gagccaggcc ctgagcaggc agccacaccg ccagtgtggg aacgtggagg
     7441 ggctggaggg atgcagcagg gctcctcccc agccccagac agctgccagc ctggccccgg
     7501 acccagccct ggcctgacca gcatagtctc cgggaccagc gaggacctgc ggcctcccag
     7561 acgacgccca cctccaggga agcaaatccc ttgctccagc cctggctgct gcctcagttt
     7621 tcccagcgtc cgtgacctgg cacagcatct gcgaacccac tgcccgccca cacagtccct
     7681 ggaaggcaag ctcttccgct gctcagccct gagctgcacc gagaccttcc ccagcatgca
     7741 ggagctggtg gctcacagca aactgcacta caaacccaat cgctacttca agtgtgagaa
     7801 ctgcctcctg cgcttccgca cgcaccgctc gctcttcaag cacctgcatg tttgcgcgga
     7861 gcatgcgcag agcccagccc cgccgccacc cccggccctg gaccgagagc cgcccgcgcc
     7921 ggagcgcccc ccggaggttg accccgcatc agcgccgggc ctgccgttcc cgctgctgga
     7981 acccttcacg acccccgccc ctgcccccac cggaccgttc ctgccctact tgaaccctgc
     8041 gccctttggc ctaagccccc cgcgcctgcg ccccttcctg gccgctgcac ccgggccgcc
     8101 ggcttccagc gccgccgtct ggaaaaagag ccaaggtgct ggcagcagcc cccgaagacc
     8161 ccagggcggc tccgacgcgc cctcaggtgc gtgcaggtag taatgaacta gtccgtaact
     8221 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaca ttcttcagcg
     8281 cataaagatg ccacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8341 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8401 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8461 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8521 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8581 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8641 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8701 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8761 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8821 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     8881 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     8941 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     9001 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9061 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9121 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9181 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     9241 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     9301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9361 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     9421 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9481 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9541 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     9601 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9661 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9721 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9781 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10081 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10141 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
    10201 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcacttt
//
