LOCUS       sequence-466922-       14155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P element vector for transposon insertion of mei-S332-GFP 5.6kb 
            genomic DNA (T331D) into Drosophila genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14155)
  AUTHORS   Nogueira C, Kashevsky H, Pinto B, Clarke A, Orr-Weaver TL
  TITLE     Regulation of centromere localization of the Drosophila Shugoshin 
            MEI-S332 and sister-chromatid cohesion in meiosis.
  JOURNAL   G3 (Bethesda). 2014 Jul 31;4(10):1849-58. doi: 
            10.1534/g3.114.012823.
  PUBMED    25081981
REFERENCE   2  (bases 1 to 14155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.114"; 
            journalName: "G3 (Bethesda)"; date: "2014-07-31- 31"; volume: "4"; 
            issue: "10"; pages: "1849-58"
COMMENT     Sequence Label: mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D
FEATURES             Location/Qualifiers
     source          1..14155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(824..854,855..1409)
                     /label=P element 5' end
     CDS             complement(join(2188..2802,2873..3004,3208..3523,3585..4239,
                     4314..4587,4989..5060))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     5512..5530
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             6829..7545
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(6862..6890)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7485..7505
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     regulatory      10500..10509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(11985..12186,12187..12217)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(12755..12774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12874..12896
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12934..12952)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13020..13124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13125..13193,13194..13985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13343..13362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccttctt
      601 cttgaactcg ggctcggtgc cagtatacct caaatggttg tcgtacctct catggttccg
      661 ttacgccaac gagggtctgc tgattaacca atgggcggac gtggagccgg gcgaaattag
      721 ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa
      781 cttctccgcc gccgatctgc cgctggacta cgtgggtctg gcccatgatg aaataacata
      841 aggtggtccc gtcgatagcc gaagcttacc gaagtataca cttaaattca gtgcacgttt
      901 gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat taacccttac
      961 gtggaataaa aaaaaatgaa atattgcaaa ttttgctgca aagctgtgac tggagtaaaa
     1021 ttaattcacg tgccgaagtg tgctattaag agaaaattgt gggagcagag ccttgggtgc
     1081 agccttggtg aaaactccca aatttgtgat acccacttta atgattcgca gtggaaggct
     1141 gcacctgcaa aaggtcagac atttaaaagg aggcgactca acgcagatgc cgtacctagt
     1201 aaagtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg gagtacacaa
     1261 acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt
     1321 taggtcctgt tcattgttta atgaaaataa gagcttgagg gaaaaaattc gtactttgga
     1381 gtacgaaatg cgtcgtttag agcagcagcc gaattaattc tagttccagt gaaatccaag
     1441 cattttctaa attaaatgta ttcttattat tatagttgtt atttttgata tatataaaca
     1501 acactattat gcccaccatt tttttgagat gcatctacac aaggaacaaa cactggatgt
     1561 cactttcagt tcaaattgta acgctaatca ctccgaacag gtcacaaaaa attaccttaa
     1621 aaagtcataa tattaaatta gaataaatat agctgtgagg gaaatatata caaatatatt
     1681 ggagcaaata aattgtacat acaaatattt attactaatt tctattgaga cgaaatgaac
     1741 cactcggaac catttgagcg aaccgaatcg cgcggaacta acgacagtcg ctccaaggtc
     1801 gtcgaacaaa aggtgaatgt gttgcggaga gcgggtggga gacagcgaaa gagcaactac
     1861 gaaacgtggt gtggtggagg tgaattatga agagggcgcg cgatttgaaa agtatgtata
     1921 taaaaaatat atcccggtgt tttatgtagc gataaacgag tttttgatgt aaggtatgca
     1981 ggtgtgtaag tcttttggtt agaagacaaa tccaaagtct acttgtgggg atgttcgaag
     2041 gggaaatact tgtattctat aggtcatatc ttgtttttat tggcacaaat ataattacat
     2101 tagctttttg agggggcaat aaacagtaaa cacgatggta ataatggtaa aaaaaaaaaa
     2161 caagcagtta tttcggatat atgtcggcta ctccttgcgt cgggcccgaa gtcttagagc
     2221 cagatatgcg agcacccgga agctcacgat gagaatggcc agacccacgt agtccagcgg
     2281 cagatcggcg gcggagaagt taagcgtctc caggatgacc ttgcccgaac tggggcacgt
     2341 ggtgttcgac gatgtgcagc taatttcgcc cggctccacg tccgcccatt ggttaatcag
     2401 cagaccctcg ttggcgtaac ggaaccatga gaggtacgac aaccatttga ggtatactgg
     2461 caccgagccc gagttcaaga agaagccgcc aaagagcagg aatggtatga taaccggcgg
     2521 acccacagac agcgccatcg aggtcgagga gctggcgcag gatattagat atccgaagga
     2581 cgttgacaca ttggccacca gagtgaccag cgccaggcag ttgaagaagt gcagcactcc
     2641 ggcccgcagt ccgatcatcg gataggcaat cgccgtgaag accagtggca ctgtgagaaa
     2701 aagcggcaat tcggcaatcg ttttgcccag aaagtatgtg tcacagcgat aaagtcgact
     2761 tcgggcctcc ctcataaaaa ctggcagctc tgaggtgaac acctaaatcg aatcgattca
     2821 ttagaaagtt agtaaattat tgaaatgcaa atgtattcta aacatgactt acatttatcg
     2881 tggcaaagac gttttgaaag gtcatgttgg tcaggaagag gaagatggct ccgttgatat
     2941 tcatcacacc cacttgcgtg agttgttggc ccaaaaagat gaggccaatc aagatggcaa
     3001 ccatctgcaa attaaaatgt tactcgcatc tcattaatat tcgcgagtta aatgaaattt
     3061 atttatcttc tgcaaaacta taaactatac atctcattga aaaaaactaa gaagggtgtg
     3121 gaatcaggca attctatcta aaatctagcg aatttgtttc caagaattgt aagcgttata
     3181 tcatttgttt ccactggaac cactcaccgt tgtctgaata agtcgcactt ttacgaggag
     3241 tggttccttg agcaccgaca gccaggatcg ccacaggacc gcccggaact gcatgaacca
     3301 ggtggccttg taggtgtacc cattctccgg ctgctccagt ggcttctcca gatttttggt
     3361 ggccaacaac tgctccatat cccgggctac tttgctaatg gcaaaattgt cgcatatctt
     3421 ggcgatccga tcacgggact cgatctcccg tccgggcaca acggccaaca cctgtacgta
     3481 aaagtccgcc ggattgtagt tggtaggaca ctgggcaccc acgctggata ggagttgaga
     3541 tgttatgtaa tactagatac ccttaataaa cacatcgaac tcactaggaa aagaagtcga
     3601 cggcttcgct gggagtgccc aagaaagcta ccctgccctc ggccatcaga aggatcttgt
     3661 caaagagctc aaacagctcg gaagacggct gatgaatggt caggatgacg gtcttgccct
     3721 tctgcgacag cttcttcagc acctggacga cgctgtgggc ggtaaaggag tccagtccgg
     3781 aggtgggctc atcgcagatc agaagcggcg gatcggttag agcctcggag gcgaatgcca
     3841 gacgcttcct ttctccgccg gacagacctt tcaccctgcc gggcacaccg atgatcgtgt
     3901 gctgacattt gctgagcgaa agctcctgga tcacctgatc cacgcgggcc actcgctgcc
     3961 gataggtcag atgtcgtggc atccgcacca tggcttggaa aatcaggtgt tccctggccg
     4021 ttagggagcc gataaagagg tcatcctgct ggacataggc gcacctggcc tgcatctcct
     4081 tggcgtccac aggttggcca ttgagcagtc gcatcccgga tggcgatact tggatgccct
     4141 gcggcgatcg aaaggcaagg gcattcagca gggtcgtctt tccggcaccg gaactgccca
     4201 tcacggccaa aagttcgccc ggataggcca cgccgcaaac tgagtttcaa attggtaatt
     4261 ggacccttta ttaagatttc acacagatca gccgactgcg aatagaaact caccgttctt
     4321 gagcaaatgt ttcctgggcg ccggtatgtg tcgctcgttg cagaatagtc cgcgtgtccg
     4381 gttgaccagc tgccgccatc cggagcccgg ctgattgacc gccccaaaga tgtccatatt
     4441 gtgccaggca taggtgaggt tctcggctag ttggccgctc cctgaaccgg agtcctccgg
     4501 cggactgggt ggccggagcg tgccgtagtt tttggcctgc ccgaagccct ggttaatgca
     4561 gctctgcgaa gccgctccgc tgtcaccctg caatgatagg ggatctcaaa tatcaactac
     4621 aagcgttatg ctcatctaac cccgaacaaa aagtaccccg aagtatccta cgaagtaggt
     4681 ttatactttt atttattttt tgtgcatcta ggatcagctt aaaatatctg gttgttatat
     4741 tttttgtaaa aaagaatata gtcgaaaatg aatgccttta gatgtcttga tcatgatatg
     4801 atctcaaaaa ttgtcttata tagcgagaac agctaccaga ataatctgtt tcgtgtcact
     4861 atttgtttgt gcaattgcgg tttgggattt ttgtgggtcg cagttctcac gccgcagaca
     4921 atttgatgtt gcaatcgcag ttcctataga tcaagtgaac ttaagatgta tgcacatgta
     4981 ctactcacat tgttcagatg ctcggcagat gggtgtttgc tgcctccgcg aattaatagc
     5041 tcctgatcct cttggcccat tgccgggatt tttcacactt tcccctgctt acccacccaa
     5101 aaccaatcac caccccaatc actcaaaaaa caaacaaaaa taagaagcga gaggagtttt
     5161 ggcacagcac tttgtgttta attgatggcg taaaccgctt ggagcttcgt cacgaaaccg
     5221 ctgacaaaat gcaactgaag gcggacattg acgctacgta acgctacaaa cggtggcgaa
     5281 agagatagcg gacgcagcgg cgaaagagac ggcgatattt ctgtggacag agaaggaggc
     5341 aaacagcgct gactttgagt ggaatgtcat tttgagtgag aggtaatcga aagaacctgg
     5401 tacatcaaat acccttggat cgaagtaaat ttaaaactga tcagataagt tcaatgatat
     5461 ccagtgcagt aaaaaaaaaa aatgtttttt ttatctactt tccgcaaaaa tgggttttat
     5521 taacttacat acatactaga attcggtacc gaagaggttc ctgaacttgc cataaattac
     5581 caaatttctg ggcaccgtgc gaaatagaat tgataaggtc gctcgataag tccgtaccac
     5641 ttcggattct gagtgctgag tgatgcactc aatcgactaa atgggtacaa agccaaacga
     5701 gttggattta caaacagact caccttgaag cgggtttcgg ttttcgactg cggtcgtagc
     5761 ttctgtgaat gaaattctcg gttctctggg ctgctggctg tcttcggtgt ttcagcaggt
     5821 gattgtcgtg attctctggc ggagatgaaa cagcatagca agcggttccc taatgtattt
     5881 ttccgaaact atggtgtata atccgtatcc gtgttcctaa gttaagttaa tccgcgcgct
     5941 agatccgtct atcgtttttc gagtgcacag gtgttgcatg tgatgtgtat cgtgtcctgt
     6001 gagtgcgggg ggaaagtccc tcccactgtt ggtaagaatt gtagaaatgt gtaaaagcct
     6061 ggctaaatgc ctaaaagacg tacaacgacg atacaaatcc gactgatttt catttttaca
     6121 caaacacacg cacactgcga cagcacactc tcactcacgt acacgcacgc acacactgcc
     6181 aggtgggctg acttctgatt cagtgcgatt ttcgcgttct gcaaggtgta tctacaacct
     6241 cccgaaacgc gtattcactt cgatttccgg gttctagcgc tgcagcattg tttatctacc
     6301 acggatatcc agattttctt cgcaatcggt cgagaaaaca ataaatttat tgtgcagctt
     6361 agaggtgtaa gagaaacata gacgtatcga tgtctctcga tggtttgtgc ctatcgaaat
     6421 cggtgtcccg caatatacac agcttccact aagcgaaatt ttagcatttt ggcatacgtg
     6481 tagaatttaa atggaaaatt acccttggta acgcatgtac gatcaaattc gcaccaactc
     6541 aacggttcct aattcttgtc gtgcaaatga gacaaacaaa tttataatta ttaaaataaa
     6601 atatattcac atcggattgt attgtttctt atatatatgg cccaaaatta aagaatttca
     6661 ttttaattaa gagcatggtc ggcgttgcca tactttcaaa aagttttcct taagctgggg
     6721 tatattccaa tagcacaaca acggtcactc tatttcaaaa ttcaaaacaa gctattggcg
     6781 tcgtctgtct ggattttgtg tatggaataa gtagatcaga agaagcaaat gggatccaaa
     6841 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     6901 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     6961 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     7021 ttctcttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt
     7081 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     7141 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     7201 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     7261 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     7321 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     7381 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     7441 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     7501 gtaacagctg ctgggattac acatggcatg gatgaactat acaaaagatc caaagtggag
     7561 cagcagtaca agctgctgaa tgcggagcta atggaccaag tgcagaagca gcgcttggag
     7621 atcggcgagt acaggaagcg ggtgatatct ttggagcgag agatcatgga cattcgggag
     7681 gagcacgtcc tgcaaaacca tcgccagcgc atggagaaca tcagtatcgt acgcagcctg
     7741 atgctgagcc tgaacgtgga ctccgattcc ctggcagtgc gtcaggagcc cgctcccgca
     7801 gctcagataa acagaccaag cggcccgcgt cgcagctcca gggaaatctg caaggacatg
     7861 cgacggacgt gtgcgttggc ccgcaccacc agaccaatct cgccaagaag atcttcctca
     7921 gtgaccagta ctgtatcgtc cactagtcgc cgctccagcg cagaagtgca gtccgaggtg
     7981 gtgaccacta ggataccaga ggatagaaga gccaacaagg taacttatac ttaaatttga
     8041 ttatgctttt cttaacgatt cattatcggc ttgagtacag cctacaccac cgccacgaag
     8101 accagcggaa ttagtgtttg atgaggacga ctcggacgat gatttcgacg aagcagtaag
     8161 ccccgtggag gagacacaga ctgagcaaaa tgaagagaac aatcgtctgt ttagcattat
     8221 agaggaaaat ggcagtgaag gggagtccac agactcgtca tcatcatgcg aggccatcta
     8281 ttgcgacaca acgtttgaaa gtagtccgcc aaacgcccaa gtcaccgtca cgcccagtgg
     8341 acgggctctg cgggaagtgg acaccaatat ccctgtagca gtgtccctca gtcggggaaa
     8401 agagactggg aagggatcat ggttggctat ttccgtagct gtggaagatt ctccacagga
     8461 gccaagcata cagtgcccac gactggcagt caccaggcct agtcaatcgt ctggaatatt
     8521 tcctgatgtc aacggattaa cgccgcgaag aagcctgttc aatgggatag gcaaaatggc
     8581 cggaagcacc agcgatccga aatcctttct cgtcgaggaa atgccatcta taagaactag
     8641 aagccgaact gcggctaata agaaatcaga aaatacggat atgtctagtt ctttctgcaa
     8701 caacagcgca aggccgagta gaagctgcag gcccaccagt ttggtggagc caagtctgaa
     8761 gaacaagttg cgcaatggct cgaaaggcaa ggccaaggcc aaaaagtaga tagacgaatt
     8821 ttttaatagg ctttaatgtt gtttctgtaa tttcattggt tttcaatacc actttttacc
     8881 atttttaata tgaataatat ttttaaatcg tcaggccctg ataagtttaa tttatagact
     8941 aaaagctgac atcggattac tttttgcctg caatagaggg catattcagt tcatccagcg
     9001 ctatcggaag gaagacactc catcatcatc atcacccaaa gatgccaata ggaatttaaa
     9061 agtacgtcta atttttacat gttcatttgt gaataaaaca acgtttcaat taattatttg
     9121 ttaatacgct tttaggggaa ctcattttat agattcctct tagatgcaaa ctacgtttat
     9181 tcgcacaaag ttgttggaaa catttacaaa agactctgaa gaacaaagta caattacaat
     9241 tacaattaaa tacagtagca aaagggtaat gattatacgt aggtaggcga taattgcact
     9301 tcttttagac tacctcgaac ttgcccatac aacagttgag atggtctcct gcatggttgt
     9361 ggcttggctg ctccgtgttc tccttgatct cgctgataat gtcctgtatc ttgttgatga
     9421 tctgttgcga gttttcacct tcgccaggag tgtgttcgct acctgcggcc gcttctggtt
     9481 gctttgcctg ggcggcatct gcgggtagct ccacgcccaa actgaccacc agcttgttta
     9541 gtgcctcaat cttctcgcgc agctgctctc gctccgcttg caagagacgc atttgggcgg
     9601 ccttctcgta ctcacgctgc tttagcatgc gcctctgcgt ttgatagagg gcaatgtact
     9661 cgccaatcgt ttcggtttcg ctctgcagct ggagcaccag atgctccagg ctgtgcttct
     9721 cctccgtcaa gtcggcgacc tggctgatca gtgtggtgaa acgcgattgc aagcgttcca
     9781 cggcctcggc ggtgggcagc gtttcagctg cttgtctgtg ggcgtggttc tggccgtgat
     9841 catgtggatg atcatgctga tgatcgtgtg gatggctatg tgaatgatca tgtgtgtgat
     9901 cgtgcggatg atcatgtgga tgaccatgat caccagcatt atgatggtta tgttcatggg
     9961 aatgatcgtg gtcattatca tgacaatgac cgtcggaatg ctgatgagag tgatcgtgcg
    10021 aatgattatg caaaagacca acttccttcg cttcctctgc ttcgttttcc ggtgcatctg
    10081 ctccattctc tttttcggtt tcctcaccgg cgtgataagc ttttgcctga aagaaatgat
    10141 aatgggatat aaagtcagtt aaattttaat cccagtccaa cccacctcat aatgcctgag
    10201 acggtccagc tgctgttgca acattaactg ctgacgttgt agttccacgt tctcgtgcga
    10261 tagtctaatc atttcctcat ccttgaactt aaagcgctcc tcatttgctt gaagcttttg
    10321 ctccatgctg ttgtagttct ggcggatttc tcggttggca aactcttctg aatccaagcg
    10381 gatgaccagc tcggctttgt cgtttgtcaa ctgaacaaat cgctgctgca gttcgtccaa
    10441 ctggtttttc atctctacat tctgggttag ggcacgcgat gcggccactt tgtctgactc
    10501 catggtggcc aacagcttgg actgatcggg ctgctcagct tgcaagcgtt cgagttgctt
    10561 ttccaactcg tctaaagccg agttctttaa ctgcagctga tcgtcctgcg acttgatctt
    10621 cagctggaat tcgtggcgct caaactcgaa gttttcgatc tgctctttca agacagccag
    10681 ttcatctttt aaggctttct gaaattgaac taggagatta aacaggtttt ccatattttt
    10741 ttggcttact tacctgctcg ctggtttctt ccttggatgg ctgcacttga ctggcctctt
    10801 taaattgggc ctgcaggctg atgttctttt gtatatcccg ctccagggcg cccacgtgct
    10861 ccacaagctg gcgttctcgc tccctcagtg agttcagtac ctctgagagt tctgtgtttc
    10921 gttcgttgag cttggcgatc tcgccactct gatgctgcac atagttttga tactgaccag
    10981 ctgcctgctc cttgtcctgc gttagttgac ccaccatcgc ttggaggtcg cgtatctgtt
    11041 gctcgtacat gtattgtgtt tgcaccagcg actccactcg gttgtctgtg gtgacgctag
    11101 actcgtctga gagctgccgc agcttcagct gcgccatctc gagttcgttc gatttttggg
    11161 aaagctgttg ctgcagttcc tcttgatgct ggttgctctg ctttagctgg gcctctagtt
    11221 cgttgagatg cgactgttgg tccttgattt gtgcctgcga ttgtagcagt tgtgcctgga
    11281 gttgcgcgta ctgctgtcgc tgctgctcac cggtcgactg ttgatcctgg cagtgctgtt
    11341 gtagctgctc cagacgcaat tgattctgat ccttgaggat gcgaagctcc tccagctcta
    11401 gctcacgctc ccggcaagcc aggcgaacct tggtcagctc cgcattgagc tgggcctttt
    11461 cagcgatgat cacctggatg gcctgcaagc tagaggcatc tgccagtcca ccattgtgtt
    11521 ccgactcgtt ttgggcaaaa taagaagcgg ctgtgggcag ggcagactgc tccggcggct
    11581 gcgtcccacc tgctcctcca ccgttcacat ttatctcgct gtctgaccgt tccgacagat
    11641 tgctggatat gctcacagtg gacgagtgcg attgcgtggt ggcagccacc ggctcctctg
    11701 ctcccggctg cccattgttg ttgctggcgc gctgctggta ctccttcagc tgtgtccaca
    11761 gaagcgtgtt aaacaaaatc aatattagtc ggccaatatg tgtccacgtc acaagagcaa
    11821 aacaattgtg tgtggcactg gttgtcgcac ctttttcctt gccgcggcca acttctgcgc
    11881 cttggcatcc tgcgtgtcat cgggcatgtc cttcggggtc agcagcggta cccgcccggg
    11941 gatcagatcc gcggccgcct cgaggtcgac ctgcagccaa gctttgcgta ctcgcaaatt
    12001 attaaaaata aaactttaaa aataatttcg tctaattaat attatgagtt aattcaaacc
    12061 ccacggacat gctaagggtt aatcaacaat catatcgctg tctcactcag actcaatacg
    12121 acactcagaa tactattcct ttcactcgca cttattgcaa gcatacgtta agtggatgtc
    12181 tcttgccgac gggaccacct tatgttattt catcatggtc tggccattct catcgtgagc
    12241 ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca
    12301 tatatccgaa ataactgctt gttttttttt tttaccatta ttaccatcgt gtttactgtt
    12361 tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca agatatgacc
    12421 tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg atttgtcttc
    12481 taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat cgctacataa
    12541 aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc ttcataattc
    12601 acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
    12661 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
    12721 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    12781 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    12841 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    12901 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
    12961 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    13021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    13081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    13141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    13201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    13261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    13321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    13381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    13441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    13501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    13561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    13621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    13681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    13741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    13801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    13861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    13921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    13981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    14041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    14101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
