LOCUS       sequence-46698-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ326 - operon for germline expression
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(151..474)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    480..603
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(674..691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(674..690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(683..705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      754..840
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttcttctcct ttactcatga tgcgttgaag cagtttccct gaattaaaat tagaagttga
       61 aaatacaata gaaagaaaat tggaaggtat ataaactcgc aaaaacgagg aaatggcaga
      121 ttcaataaga tgaataaata tacaacctga gacttttttc ttggcggcac aataaagttt
      181 cctctttttg ttgggtcact caacgaacac aatacaaata tttgagcgct cgcatttttc
      241 tcaacctttc gggtttaaag cacttcaaaa tggaattgaa gagagattgt gaaagtacat
      301 cagacagaat cacattttga tagcattcac ttcactcaga tgcaaacttt tcagtgacta
      361 taaaaagtca taaaataata acattaaaaa agcaaataaa ttagcgagag aattttttga
      421 caaaaagaaa gaagagtgat agagaagaag ggaatgcttg aaaggatttt gcatttatcc
      481 cactttgtac aagaaagttg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      541 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tgcagtcact
      601 atgaaccaac tacttagatg gtattagtga cctgtagaat tcgagctcta gagctgcagg
      661 gcccgagctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac atccatgcta
      721 gcgttaacgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa
      781 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
      841 ccgccgggag cggat
//
