LOCUS       sequence-46707-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pAAVscCBPTuDmiR122 Gpluc
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     126..145
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
       61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
      121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg ggtttatata
      181 tcttgtggaa aggacgcggg atccgacggc gctaggatca tcaaccaaac accattgatc
      241 ttcacactcc acaagtattc tggtcacaga atacaaccaa acaccattga tcttcacact
      301 ccacaagatg atcctagcgc cgtctttttt aagctgtccc ggatccggtg gtggtgcaaa
      361 tcaaagaact gctcctcagt ggatgttgcc tttacttcta ggcctgtacg gaagtgttac
      421 ttctgctcta aaagctgcgg aattgtaccc gcggccgatc caccggtcgc caccatctag
      481 catgggagtc aaagttctgt ttgccctgat ctgcatcgct gtggccgagg ccaagcccac
      541 cgagaacaac gaagacttca acatcgtggc cgtggccagc aacttcgcga ccacggatct
      601 cgatgctgac cgcgggaagt tgcccggcaa gaagctgccg ctggaggtgc tcaaagagat
      661 ggaagccaat gcccggaaag ctggctgcac caggggctgt ctgatctgcc tgtcccacat
      721 caagtgcacg cccaagatga agaagttcat cccaggacgc tgccacacct acgaaggcga
      781 caaagagtcc gcacagggcg gcataggcga ggcgatcgtc gacattcctg agattcctgg
      841 gttcaaggac
//
