LOCUS       sequence-46708-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pAAV TBG FFluc miR122sponge
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(322..341)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    387..442
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
ORIGIN
        1 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
       61 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaacaaa caccatacaa
      121 cactccacaa acaccataca acactccaca aacaccatac aacactccac aaacaccata
      181 caacactcca caaacaccat acaacactcc acaaacacca tacaacactc cacaaacacc
      241 atacaacact ccagcgcgtg gtacctctag agtcgacccg ggcggcctcg aggacggggt
      301 gaactacgcc tgaggatccg atctttttcc ctctgccaaa aattatgggg acatcatgaa
      361 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
      421 ttggaatttt ttgtgtctct cactcggaag caattcgttg atctgaattt cgaccaccca
      481 taatacccat taccctggta gataagtagc atggcgggtt aatcattaac tacaaggaac
      541 ccctagtgat ggagttggcc actccctctc tgcgcgctcg
//
