LOCUS       sequence-467125-       10110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication 
            origin and URA selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 10110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM07
FEATURES             Location/Qualifiers
     source          1..10110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(129..717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(209..228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             1556..1567
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1574..2287
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2299..2370
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2386..2413
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        2427..2502
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2636..2978
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2989..3786
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="SKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLE
                     LVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQY
                     SAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEY
                     TKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDV
                     VSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      3801..3998
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(4149..4940,4941..5009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4772..4791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5010..5114)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5817..9920
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5817..9920
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9930..9950
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9966..9989
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      join(9993..10110,1..72)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
       61 ggacgctcga agtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      721 gatcctttga tcttttctac actagttcaa catctttgga taatatcaga atgagaaaga
      781 acagatacgc agtacgtttt ttggtgagct ctttgcactt ctttagttct ttccatcaat
      841 atcagttttt taaactttta ggactaaaag tgatgtttaa cttcaaaatg tttaaaattt
      901 tgttcttccc gacgttcatt aagaatacta atacacttta ataattattt taatattttg
      961 ttctaaataa tgacttttaa ttaaaaaaga taaaatataa aaacatcata ataactcacc
     1021 agaggttaag aacaaaaaaa caaattagat atctgctaat ccaatatagt taaatcaatc
     1081 tttccttggt ataatgggta tattacatat atttcaagga ccgacactcc taccaaatat
     1141 ctaaaattta ccatattaac ataacatgta tataaacgtc aaatcataat cagcactagt
     1201 atatgtgtta tgtagtatac tctttcttca acaattaaat actctcggta gccaagttgg
     1261 tttaaggcgc aagactgtaa tttatcacta cgaaatcttg agatcgggcg ttcgactcgc
     1321 ccccgggaga gatggccggc atggtcccag cctcctcgct ggcgccggct gggcaacacc
     1381 ttcgggtggc gaatgggact cgagaccgaa agtgaaacgt gatttcatgc gtcattttga
     1441 acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt
     1501 ttcttaacat cgcggcaact caagaaacgg caggttcgga tcttagctac tagagaaaga
     1561 ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
     1621 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
     1681 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggttcc
     1741 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
     1801 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
     1861 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
     1921 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagagg acggcaatat
     1981 cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa
     2041 acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt
     2101 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
     2161 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
     2221 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
     2281 gtacaaatga ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2341 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2401 gggcctttct gcgtttatag gtctccgttt tagagctaga aatagcaagt taaaataagg
     2461 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta ttttttgtca
     2521 ctattgttat gtaaaatgcc acctctgaca gtatggaacg caaacttctg tctagtggat
     2581 ataggtctag agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2641 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2701 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     2761 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     2821 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     2881 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     2941 aggatacagt tctcacatca catccgaaca taaacaacaa tggagacgtc gaaagctaca
     3001 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     3061 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     3121 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     3181 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     3241 tttttactct tcgaggacag aaaatttgct gacattggta atacagtcaa attgcagtac
     3301 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     3361 ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     3421 ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     3481 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     3541 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     3601 ttagatgaca agggagatgc attgggtcaa cagtatagaa ccgtggatga tgtggtttct
     3661 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     3721 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     3781 caaaaccgtc tctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat
     3841 ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata
     3901 ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat
     3961 catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc
     4021 gccatccagt gtcgaaaacg agctctcgag aacccttaat caaaaaggat cttcacctag
     4081 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4141 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4201 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4261 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc agatttatca
     4321 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4381 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4441 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4501 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4561 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4621 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4681 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4741 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4801 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4861 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4921 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4981 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5041 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5101 ataggggttc gtcgacagtc gaacaagaag caggcaaagt ttagagcact gcccctccgc
     5161 actcaaaaaa gaaaaaacta ggaggaaaat aaaattctca accacacaaa cacataaaca
     5221 catacaaata caaatacaag cttatttact tgacatcgcg cgatcttcca ctattcagcg
     5281 ccgtccgccc tctctcgtgt tttttgttta cgcgacaact atgcgaaatc cggagcaacg
     5341 ggcaaccgtt tggggaaaga ccacacccac gcgcgatcgc catggcaacg aggtcgcaca
     5401 cgccccacac ccagacctcc ctgcgagcgg gcatgggtac aatgtccccg ttgccacaga
     5461 caccacttcg tagcacagcg cagagcgtag cgtgttgttg ctgctgacaa aagaaaattt
     5521 ttcttagcaa agcaaaggag gggaagcacg ggcagatagc accgtaccat acccttggaa
     5581 actcgaaatg aacgaagcag gaaatgagag aatgagagtt ttgtaggtat atatagcggt
     5641 agtgtttgcg cgttaccatc atcttctgga tctatctatt gttcttttcc tcatcacttt
     5701 cccctttttc gctcttcttc ttgtctttta tttctttctt ttttttaatt gttccctcga
     5761 ttggctatct accaaagaat ccaaacttaa tacacgtatt tatttgtcca attaccatgg
     5821 ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca
     5881 ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
     5941 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga
     6001 ctcgactcaa acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc
     6061 tacaggagat tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg
     6121 aagagtcttt tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata
     6181 tagtagatga agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat
     6241 tggtagattc tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga
     6301 ttaagtttcg tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg
     6361 acaaactatt tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta
     6421 acgcaagtgg agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat
     6481 tagaaaatct cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca
     6541 ttgctttgtc attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg
     6601 ctaaattaca gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa
     6661 ttggagatca atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac
     6721 tttcagatat cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga
     6781 ttaaacgcta cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac
     6841 aacttccaga aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt
     6901 atattgatgg gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa
     6961 aaatggatgg tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc
     7021 aacggacctt tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta
     7081 ttttgagaag acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa
     7141 aaatcttgac ttttcgaatt ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt
     7201 ttgcatggat gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg
     7261 tcgataaagg tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc
     7321 ttccaaatga aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata
     7381 acgaattgac aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag
     7441 gtgaacagaa gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta
     7501 agcaattaaa agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag
     7561 gagttgaaga tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta
     7621 aagataaaga ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa
     7681 cattgacctt atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc
     7741 tctttgatga taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt
     7801 tgtctcgaaa attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt
     7861 ttttgaaatc agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt
     7921 tgacatttaa agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg
     7981 aacatattgc aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa
     8041 aagttgttga tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg
     8101 aaatggcacg tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga
     8161 aacgaatcga agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg
     8221 aaaatactca attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca
     8281 tgtatgtgga ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatcacattg
     8341 ttccacaaag tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata
     8401 aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact
     8461 attggagaca acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga
     8521 aagctgaacg tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg
     8581 ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta
     8641 aatacgatga aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat
     8701 tagtttctga cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc
     8761 atcatgccca tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc
     8821 caaaacttga atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga
     8881 ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata
     8941 tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc
     9001 taatcgaaac taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca
     9061 cagtgcgcaa agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga
     9121 caggcggatt ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc
     9181 gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt
     9241 cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag
     9301 agttactagg gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt
     9361 tagaagctaa aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata
     9421 gtctttttga gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa
     9481 aaggaaatga gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt
     9541 atgaaaagtt gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc
     9601 ataagcatta tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt
     9661 tagcagatgc caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa
     9721 tacgtgaaca agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg
     9781 ctgcttttaa atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag
     9841 ttttagatgc cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt
     9901 tgagtcagct aggaggtgac ggtggagggc caaaaaagaa aagaaaagtt gaagatgctt
     9961 ctgctcatca tcaccatcac catcaccatt aaatccgctc taaccgaaaa ggaaggagtt
    10021 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
    10081 ttatttatat ttcaaatttt tctttttttt
//
