LOCUS       sequence-467131-       10833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication 
            origin and split-Hyg selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM06
FEATURES             Location/Qualifiers
     source          1..10833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(129..717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(209..228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             1556..1567
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1574..2287
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2299..2370
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2386..2413
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        2427..2502
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2636..2978
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      4524..4721
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(4872..5663,5664..5732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5495..5514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5733..5837)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6540..10643
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6540..10643
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10653..10673
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10689..10712
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      join(10716..10833,1..72)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
       61 ggacgctcga agtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      721 gatcctttga tcttttctac actagttcaa catctttgga taatatcaga atgagaaaga
      781 acagatacgc agtacgtttt ttggtgagct ctttgcactt ctttagttct ttccatcaat
      841 atcagttttt taaactttta ggactaaaag tgatgtttaa cttcaaaatg tttaaaattt
      901 tgttcttccc gacgttcatt aagaatacta atacacttta ataattattt taatattttg
      961 ttctaaataa tgacttttaa ttaaaaaaga taaaatataa aaacatcata ataactcacc
     1021 agaggttaag aacaaaaaaa caaattagat atctgctaat ccaatatagt taaatcaatc
     1081 tttccttggt ataatgggta tattacatat atttcaagga ccgacactcc taccaaatat
     1141 ctaaaattta ccatattaac ataacatgta tataaacgtc aaatcataat cagcactagt
     1201 atatgtgtta tgtagtatac tctttcttca acaattaaat actctcggta gccaagttgg
     1261 tttaaggcgc aagactgtaa tttatcacta cgaaatcttg agatcgggcg ttcgactcgc
     1321 ccccgggaga gatggccggc atggtcccag cctcctcgct ggcgccggct gggcaacacc
     1381 ttcgggtggc gaatgggact cgagaccgaa agtgaaacgt gatttcatgc gtcattttga
     1441 acattttgta aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt
     1501 ttcttaacat cgcggcaact caagaaacgg caggttcgga tcttagctac tagagaaaga
     1561 ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
     1621 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
     1681 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggttcc
     1741 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
     1801 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
     1861 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
     1921 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagagg acggcaatat
     1981 cctgggccat aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa
     2041 acaaaaaaat ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt
     2101 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
     2161 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
     2221 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
     2281 gtacaaatga ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2341 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2401 gggcctttct gcgtttatag gtctccgttt tagagctaga aatagcaagt taaaataagg
     2461 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta ttttttgtca
     2521 ctattgttat gtaaaatgcc acctctgaca gtatggaacg caaacttctg tctagtggat
     2581 ataggtctag agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     2641 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2701 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     2761 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     2821 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     2881 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     2941 aggatacagt tctcacatca catccgaaca taaacaacaa tgggaaagaa accagaattg
     3001 acagctacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtgtc cgacctgatg
     3061 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3121 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3181 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt tagcgagagc
     3241 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3301 gaactgcccg ctgttctgca accggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     3361 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3421 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3481 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3541 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3601 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3661 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3721 cgctacttcg agcggaggca tccggagctt gcaggatcga cgtcgctgcc tgcaggacag
     3781 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca accggtcgcg
     3841 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     3901 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     3961 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     4021 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     4081 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     4141 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     4201 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     4261 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     4321 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     4381 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     4441 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     4501 ccgagggcaa aggaataatc agtactgaca ataaaaagat tcttgttttc aagaacttgt
     4561 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt
     4621 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa
     4681 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac
     4741 gccgccatcc agtgtcgaaa acgagctctc gagaaccctt aatcaaaaag gatcttcacc
     4801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4981 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     5041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5821 caaatagggg ttcgtcgaca gtcgaacaag aagcaggcaa agtttagagc actgcccctc
     5881 cgcactcaaa aaagaaaaaa ctaggaggaa aataaaattc tcaaccacac aaacacataa
     5941 acacatacaa atacaaatac aagcttattt acttgacatc gcgcgatctt ccactattca
     6001 gcgccgtccg ccctctctcg tgttttttgt ttacgcgaca actatgcgaa atccggagca
     6061 acgggcaacc gtttggggaa agaccacacc cacgcgcgat cgccatggca acgaggtcgc
     6121 acacgcccca cacccagacc tccctgcgag cgggcatggg tacaatgtcc ccgttgccac
     6181 agacaccact tcgtagcaca gcgcagagcg tagcgtgttg ttgctgctga caaaagaaaa
     6241 tttttcttag caaagcaaag gaggggaagc acgggcagat agcaccgtac catacccttg
     6301 gaaactcgaa atgaacgaag caggaaatga gagaatgaga gttttgtagg tatatatagc
     6361 ggtagtgttt gcgcgttacc atcatcttct ggatctatct attgttcttt tcctcatcac
     6421 tttccccttt ttcgctcttc ttcttgtctt ttatttcttt ctttttttta attgttccct
     6481 cgattggcta tctaccaaag aatccaaact taatacacgt atttatttgt ccaattacca
     6541 tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga
     6601 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
     6661 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
     6721 cgactcgact caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
     6781 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
     6841 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
     6901 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
     6961 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
     7021 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
     7081 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
     7141 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
     7201 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
     7261 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
     7321 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
     7381 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
     7441 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
     7501 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
     7561 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
     7621 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
     7681 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
     7741 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
     7801 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
     7861 aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc
     7921 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
     7981 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
     8041 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
     8101 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
     8161 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
     8221 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
     8281 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     8341 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     8401 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     8461 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
     8521 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     8581 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     8641 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     8701 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     8761 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     8821 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
     8881 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     8941 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     9001 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca
     9061 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
     9121 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     9181 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     9241 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     9301 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     9361 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     9421 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     9481 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
     9541 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     9601 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
     9661 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     9721 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     9781 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
     9841 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
     9901 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
     9961 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
    10021 aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact
    10081 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
    10141 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
    10201 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
    10261 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
    10321 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
    10381 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
    10441 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
    10501 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
    10561 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
    10621 atttgagtca gctaggaggt gacggtggag ggccaaaaaa gaaaagaaaa gttgaagatg
    10681 cttctgctca tcatcaccat caccatcacc attaaatccg ctctaaccga aaaggaagga
    10741 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
    10801 acgttattta tatttcaaat ttttcttttt ttt
//
