LOCUS       sequence-467133-        6229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC
  TITLE     Identification of specific PP2A complexes involved in human cell 
            transformation.
  JOURNAL   Cancer Cell. 2004 Feb . 5(2):127-36.
  PUBMED    14998489
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2004 Feb . 5(2):127-36."
COMMENT     Sequence Label: pLCP FLAG B55 alpha
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             3..472
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    537..736
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             937..1353
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1283..1305
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1321..1337
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1367..1390
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2760..2780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2765..3094
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2945..3080
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             3718..4187
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4317..4334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4488..5076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4568..4587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5236..6027,6028..6096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5859..5878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6097..6201)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
       61 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      121 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      181 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      241 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      301 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
      361 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      421 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      481 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggcaagc
      541 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      601 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      661 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
      721 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
      781 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
      841 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
      901 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
      961 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1021 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1081 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1141 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1201 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1261 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1321 ctttatccag ccctcactcc ttctctaggc gccggccgga tccatggact acaaagacga
     1381 tgacgacaag gcaggagctg gaggagggaa tgatattcag tggtgttttt ctcaggtgaa
     1441 aggagcagta gatgatgatg tagcagaagc agatataatt tctacagtag aatttaatca
     1501 ttctggagaa ttactagcaa caggagataa aggtggtaga gttgtcatct ttcaacagga
     1561 gcaggagaac aaaatccagt ctcatagcag aggagaatat aatgtttaca gcaccttcca
     1621 gagccatgaa ccagagtttg actacttgaa aagtttagaa atagaagaaa agatcaacaa
     1681 aattaggtgg ttaccccaga aaaatgctgc tcagttttta ttgtctacca atgataaaac
     1741 aataaaatta tggaaaatca gtgaaaggga caaaagacca gaagggtata acttgaaaga
     1801 ggaggatgga aggtatagag atcctactac agttactaca ctacgagtgc cagtctttag
     1861 gcctatggat ctaatggttg aggccagtcc acgaagaata tttgccaatg ctcatacata
     1921 tcacatcaac tcaatttcta ttaatagtga ttatgaaaca tatttatctg cagatgattt
     1981 gcggattaat ctttggcatc tggaaattac agacaggagt tttaacattg tggatatcaa
     2041 gcctgccaat atggaagagc taacagaggt gattacagca gcagaatttc atccaaacag
     2101 ctgtaacaca tttgtataca gcagcagtaa aggaactatt cggctatgtg acatgagggc
     2161 atctgccctc tgtgatagac attctaaatt gtttgaagaa cctgaagatc ccagtaacag
     2221 gtcatttttt tccgaaatca tctcctctat ttcggatgta aaattcagcc atagtggtcg
     2281 atatatgatg actagagact atttgtcagt caaaatttgg gacttaaata tggaaaacag
     2341 gcctgtggaa acataccagg tgcatgaata cctcagaagt aaactctgtt cactgtatga
     2401 aaatgactgc atatttgaca aatttgaatg ttgttggaat ggatctgaca gtgttgtcat
     2461 gactggatct tacaataatt tcttcagaat gtttgacaga aacacaaagc gagacataac
     2521 cctagaagca tcgcgggaaa acaataagcc tcgcacagtt ctgaagcctc gcaaagtctg
     2581 tgcaagtggc aagcgaaaga aagatgaaat aagtgttgac agcctagact tcaataagaa
     2641 aatccttcac acagcctggc accccaagga aaatatcatt gccgtagcta ctacaaacaa
     2701 tctgtatata tttcaagaca aagtgaatta gaattcgcca gcacagtggt cgaccctgtg
     2761 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2821 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     2881 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2941 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3001 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3061 aggaggcttt tttggaggcc taggcttttg caaaaagctt aataccatga aaacatttaa
     3121 catttctcaa caggatctag aattagtaga agtagcgaca gagaagatta caatgcttta
     3181 tgaggataat aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc
     3241 ggcagtacat attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat
     3301 tggtagtgca gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc
     3361 ttattctgac gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga
     3421 gttgatttca gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt
     3481 caaaactacg attgaagaac tcattccact caaatatacc cgaaattaaa agttttacca
     3541 taccaagctc atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     3601 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     3661 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     3721 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     3781 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     3841 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     3901 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     3961 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4021 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     4081 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     4141 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     4201 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     4261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     4321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     5041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     5101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5161 aaaaggatct tcacctagat ccttttgcgg ccgcaaatca atctaaagta tatatgagta
     5221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5341 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5521 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5581 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5821 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5941 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgca
//
