LOCUS       sequence-467176-       13930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses acoustic reporter genes or gas vesicles (ARG1) in 
            Salmonella typhimurium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13930)
  AUTHORS   Bourdeau RW, Lee-Gosselin A, Lakshmanan A, Farhadi A, Kumar SR, Nety
            SP, Shapiro MG
  TITLE     Acoustic reporter genes for noninvasive imaging of microorganisms in
            mammalian hosts.
  JOURNAL   Nature. 2018 Jan 3;553(7686):86-90. doi: 10.1038/nature25021.
  PUBMED    29300010
REFERENCE   2  (bases 1 to 13930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2018-01-3- 3"; volume: "553"; issue: "7686"; pages:
            "86-90"
COMMENT     Sequence Label: pTD103-ARG1
FEATURES             Location/Qualifiers
     source          1..13930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..39
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             1021..1032
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4320..4337
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     terminator      7835..7921
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(12177..12765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12257..12276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(12853..12947)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(12978..13772)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13089..13108)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13699..13718
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgagt
       61 taatttttaa agtatgggca atcaattgct cctgttaaaa ttgctttaga aatactttgg
      121 cagcggtttg ttgtattgag tttcatttgc gcattggtta aatggaaagt gacagtacgc
      181 tcactgcagc ctaatatttt tgaaatatcc caagagcttt ttccttcgca tgcccacgct
      241 aaacattctt tttctctttt ggttaaatcg ttgtttgatt tattatttgc tatatttatt
      301 tttcgataat tatcaactag agaaggaaca attaatggta tgttcataca cgcatgtaaa
      361 aataaactat ctatatagtt gtctttttct gaatgtgcaa aactaagcat tccgaagcca
      421 ttgttagccg tatgaatagg gaaactaaac ccagtgataa gacctgatgt tttcgcttct
      481 ttaattacat ttggagattt tttatttaca gcattgtttt caaatatatt ccaattaatt
      541 ggtgaatgat tggagttaga ataatctact ataggatcat attttattaa attagcgtca
      601 tcataatatt gcctccattt tttagggtaa ttatctagaa ttgaaatatc agatttaacc
      661 atagaatgag gataaatgat cgcgagtaaa taatattcac aatgtaccat tttagtcata
      721 tcagataagc attgattaat atcattattg cttctacaag ctttaatttt attaattatt
      781 ctgtatgtgt cgtcggcatt tatgtttttc atacccatct ctttatcctt acctattgtt
      841 tgtcgcaagt tttgcgtgtt atatatcatt aaaacggtaa tggattgaca tttgattcta
      901 ataaattgga tttttgtcac actattgtat cgctgggaat acaattactt aacataagca
      961 cctgtaggat cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa tgaattcatt
     1021 aaagaggaga aaggtaccaa gcttcgccag ggttttccca gtcgagctca tgactcatat
     1081 ttatagttat tctcctcatt tcccaagttt ccgacaacaa ataacaagga attaaaaaaa
     1141 tggcagttga aaaaaccaat tcttcctcca gcttggcaga agttattgat agaatcctcg
     1201 acaaaggtat cgtaattgac gcttgggttc gtgtttcctt agttggtatc gaactactag
     1261 caattgaagc tcggatcgtt atcgcttccg ttgaaaccta cttgaagtat gctgaagcag
     1321 ttggtttgac ccaatcagca gcagtacctg cttaatttaa ttaagcaagt taccaagtag
     1381 gagaataacc atgactcata tttatagtta ttctcctcat ttcccaagtt tccgacaaca
     1441 aataacaagg aattaaaaaa atggcagttg aaaaaaccaa ttcttcctcc agcttggcag
     1501 aagttattga tagaatcctc gacaaaggta tcgtaattga cgcttgggtt cgtgtttcct
     1561 tagttggtat cgaactacta gcaattgaag ctcggatcgt tatcgcttcc gttgaaacct
     1621 acttgaagta tgctgaagca gttggtttga cccaatcagc agcagtacct gcttaattta
     1681 attaagctcc ataatatcag gattaattaa tcctgatatt ggttctgctt tttttaatta
     1741 tctagatatt tggaaatcaa cgcttctcaa aggatattac aaaaaagtac gaatccttat
     1801 aatgtagtac ctggagaagc aatttctgtt aggtctggga gctcccatgg gatcgttatc
     1861 ccgcggccaa gcttgctagc gctaatttac tattttttgg agaacttcat gatttcttta
     1921 atggcaaaaa tccggcaaga acatcagtca atagcagaga aagtggctga actatctctt
     1981 gagaccagag aattcttgtc cgtcacgaca gcgaaaagac aagagcaagc tgaaaaacaa
     2041 gctcaagaac tgcaagcatt ctacaaggat cttcaggaaa caagtcagca gtttttatca
     2101 gaaacagccc aagccagaat tgctcaagct gaaaaacaag ctcaagaact gttagcattc
     2161 cacaaagaac ttcaagaaac aagtcagcag tttttatcag caacagccca agccagaatt
     2221 gctcaagctg aaaaacaagc gcaagaactg ttagcatttt atcaagaagt tcgggaaaca
     2281 agtcagcagt ttttatcagc aacagcccaa gcaagaattg ctcaagctga aaaacaagct
     2341 caagaactgt tagcattcca caaagaactt caagaaacaa gtcagcagtt tttatcagca
     2401 acagccgacg caagaactgc tcaagctaag gaacagaagg aatctctcct gaaattccgt
     2461 caggatttgt ttgtgagtat ctttggttaa taaagatcta actattggag gctactaaaa
     2521 atggaaatta aaaaaattat gcaagccgtg aacgactttt tcggtgaaca cgtagctcct
     2581 cctcataaaa ttacctcggt ggaagctact gaagatgaag gttggagagt tattgttgaa
     2641 gtcattgaag aacgagaata tatgaaaaaa tacgccaaag atgaaatgct cggaacgtac
     2701 gagtgctttg taaataaaga aaaagaagtc atttcattca aacgactcga cgtcagatat
     2761 agaagcgcca ttggcattga agcataaacg ataagatggc aggaggaacg taaaaatgac
     2821 cgtcttaaca gacaaaagga aaaaaggcag tggagctttt atacaagatg acgagacaaa
     2881 agaggttctt tcaagagcgc tgagctattt aaaatccggc tattccattc attttacagg
     2941 tcctgccggc ggaggcaaaa cctctttagc gcgagcgctt gctaaaaaga gaaagcgtcc
     3001 tgtaatgctg atgcacggga atcacgagct caacaacaaa gatttaattg gcgattttac
     3061 gggatacacg agcaaaaaag taatcgacca gtacgttcgt tctgtctata aaaaagatga
     3121 acaggtgagt gaaaactggc aggatggccg attgcttgaa gctgtaaaaa atggctatac
     3181 gctgatttac gacgaattta ctcgttctaa gcctgcgacg aataatatct ttctatcgat
     3241 attagaagaa ggcgtgctgc cgctgtatgg agtaaaaatg accgatcctt ttgtgcgcgt
     3301 gcatcccgat ttccgcgtca tcttcacaag caatccagct gagtatgccg gcgtatatga
     3361 tacgcaagat gcgcttctcg acaggttaat taccatgttt attgattata aagacatcga
     3421 cagagagaca gcgattttaa cggagaaaac ggacgtagaa gaagatgaag cgcgcacaat
     3481 tgtaacgctc gtagcaaacg tgcgaaaccg ctctggagac gaaaacagca gcggacttag
     3541 cctgcgggct tcgcttatga tcgctaccct tgccacgcag caagacattc ctatcgatgg
     3601 aagtgacgaa gattttcaaa cgttatgtat cgatattttg catcatccgc ttaccaaatg
     3661 tttggatgaa gaaaatgcaa aaagcaaagc cgaaaaaatc attttagaag aatgtaagaa
     3721 tatagacact gaagaaaagt aaaggagctt gaaaacatga gtgaaacaaa cgaaacaggt
     3781 atttatattt ttagcgccat tcaaacggat aaagacgaag aatttggcgc cgtggaagta
     3841 gaaggaacaa aagctgaaac atttttgatt cgctacaaag acgcggctat ggtagcagct
     3901 gaagtaccga tgaaaattta tcatcctaat cgccaaaatt tattaatgca tcaaaacgca
     3961 gtagcagcga ttatggacaa gaacgatacg gttattccaa tcagctttgg gaatgtattc
     4021 aaatcaaaag aagacgtaaa agttcttttg gaaaaccttt atccgcagtt tgaaaagctg
     4081 tttccagcga tcaaaggaaa aattgaagtc ggtttaaaag taattgggaa aaaagaatgg
     4141 cttgagaaaa aagtaaacga aaatcctgaa cttgagaaag tatcagcatc cgtaaaagga
     4201 aaatcagaag cagccggtta ttatgagcgt attcaacttg gaggaatggc tcaaaagatg
     4261 tttacttccc tgcaaaaaga agtcaagaca gatgtgtttt ctccgcttga agaagcagcg
     4321 gaagcagcaa aagcaaatga gccaacgggc gaaacgatgc ttttaaacgc gtctttctta
     4381 attaaccgag aagatgaagc gaagtttgat gaaaaagtaa atgaagcgca tgaaaactgg
     4441 aaagacaaag ccgattttca ttacagcggt ccttggcctg cttataattt tgtgaacatt
     4501 cgcctaaaag tagaagagaa ataacgtgct tcacaaatta gtaaccgcac ccattaacct
     4561 tgtagtgaaa atcggcgaaa aagtacagga agaagctgat aaacagctat atgaccttcc
     4621 gacgattcag caaaagctca ttcagcttca aatgatgttt gagcttggtg aaattccaga
     4681 agaagcgttt caagaaaaag aagatgaatt gttaatgagg tacgaaattg cgaaacgcag
     4741 agaaattgaa caatgggaag agctaacaca aaaaagaaat gaggaatcct agatgggaga
     4801 attactgtat ttatacggtt taattccaac aaaagaagca gcagccatag agccgtttcc
     4861 atcttataag gggtttgacg gagaacattc actgtaccca attgcgtttg atcaggtgac
     4921 ggctgtagtt tctaagctgg atgctgacac ctattcagaa aaagtgattc aagaaaaaat
     4981 ggagcaggat atgagctggc tgcaggaaaa agcatttcat catcacgaaa cggtagccgc
     5041 tttgtacgaa gaatttacga tcattccatt aaaattttgc accatttata aaggtgaaga
     5101 aagtctgcag gcagctattg agattaacaa agaaaagata gagaattcac tgacgctgct
     5161 tcaaggaaat gaagagtgga atgtgaaaat ttactgtgat gatacagagc ttaaaaaagg
     5221 aatcagcgaa acgaatgaaa gcgtgaaagc gaaaaaacaa gaaattagtc acttatcacc
     5281 aggaagacag ttttttgaaa agaaaaaaat agatcagctg attgaaaaag aattagagct
     5341 tcacaaaaac aaagtgtgtg aagagataca tgacaagcta aaagaattat cgctttatga
     5401 ctctgttaaa aagaattgga gcaaagacgt aactggcgca gctgaacaga tggcgtggaa
     5461 cagcgtgttt cttctcccgt ctctgcagat tactaagttc gtaaacgaaa tagaagagct
     5521 tcagcaaagg cttgaaaata aaggctggaa gtttgaagtg acgggaccat ggccgcccta
     5581 tcatttctcg agctttgcgt aaagtgagga attaacatta tgtctcttaa acaatccatg
     5641 gagaataaag atattgctct tattgatatt ttagatgtca ttttagataa aggagtcgcc
     5701 attaaaggag acttaatcat ttccatagct ggcgtcgatt tagtgtattt ggatttgcgg
     5761 gtgcttattt cttcggttga aacgcttgtg caagcaaaag aaggaaatca caaaccaatc
     5821 acttctgaac aatttgataa acaaaaggag gaattaatgg atgcaaccgg tcagccaagc
     5881 aaatggacga atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat
     5941 gacagttatt gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg
     6001 tggaacgttg acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga
     6061 aaaaatggac gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct
     6121 tggaccgcta ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca
     6181 taatatgcag tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt
     6241 tatagcgggg gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg
     6301 cttgattgtg gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga
     6361 tccgtatctc agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct
     6421 gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc
     6481 tacttaaaaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc
     6541 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc
     6601 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc
     6661 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac
     6721 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag
     6781 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc
     6841 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca
     6901 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga
     6961 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga
     7021 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca
     7081 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga
     7141 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa
     7201 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa
     7261 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa
     7321 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc
     7381 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac
     7441 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca
     7501 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat
     7561 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc
     7621 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg
     7681 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt
     7741 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa
     7801 tcaacgagta aaaagagttc ataactagag gcatcaaata aaacgaaagg ctcagtcgaa
     7861 agactgggac tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     7921 tccgccgccc tagacctagc aacaaactcc gggaggcagc gtgatgcggc aacaatcaca
     7981 cggatttccc gtgaacggtc tgaatgagcg gattattttc agggaaagtg agtgtggtca
     8041 gcgtgcaggt atatgggcta tgatgtgccc ggcgcttgag gctttctgcc tcatgacgtg
     8101 aaggtggttt gttgccgtgt tgtgtggcag aaagaagata gccccgtagt aagttaattt
     8161 tcattaacca ccacgaggca tccctatgtc tagtccacat caggatagcc tcttaccgcg
     8221 ctttgcgcaa ggagaagaag gccatgaaac taccacgaag ttcccttgtc tggtgtgtgt
     8281 tgatcgtgtg tctcacactg ttgatattca cttatctgac acgaaaatcg ctgtgcgaga
     8341 ttcgttacag agacggacac agggaggtgg cggctttcat ggcttacgaa tccggtaagt
     8401 agcaacctgg aggcgggcgc aggcccgcct tttcaggact gatgctggtc tgactactga
     8461 agcgccttta taaaggggct gctggttcgc cggtagcccc tttctccttg ctgatgttgt
     8521 cctagcgttg ctcagggcgt ctgtgttgca actaaaaaaa gtttgtcatg caatcctccc
     8581 ttaaatggca taaaaaaaga gcctagatta atccaggctc ttaaaaatca tagtcgtatt
     8641 cttcttcaat agttccagtg attttttcag tgattaactg accttcttca atcgtttctg
     8701 aagctgactt tggatcagct gttttgtgtt ctacgtagat cgatccctga tcaattttct
     8761 tcctcaaatc acgaaattct ttcttcattt cttctctcat ggcaattaat tcatcatgaa
     8821 catgacgatc tttttctcta ttctgctgtt cctctttctg ttgttgcatg tccctttcta
     8881 tgagctgaaa ggcatactct ctaaaggtac ccttactctt acctttactg aatgtactaa
     8941 tctgctctaa aacaaaatcg tacatttcat cagacatttg tgttgttcgg acattaaaga
     9001 gtggaattct tttgtttttc cccatttcta atcacctaaa ttgattgtgc ctctttttca
     9061 tttgcctgtt tctttacttg ttcagcgcgg cttaaaactt caaggccata taagttgagc
     9121 agtcttgcat cctcagcaac tatatgattt gattcagaaa tatcacggcc atatctctct
     9181 tcagtaacca cactaattga ttcttcaagc acctcgccaa caccgccaaa ataaaggtat
     9241 ttgtaaacct tatcttttgg tgcagggaac gtattttcag cttgggctat tttgatttct
     9301 gtgtattctt taagagattt tttgatgata tcagttaaat caaatttctg accggtattc
     9361 ccgtctcttc gttccatctt agtttttcca atatttgagt atataaactt ttcaagctcc
     9421 ctaacgctat caaagtgctc taggagtttt tcttttctca atttttctaa gtgcgctaaa
     9481 aacggtgctt cggtattaga aacaaaagaa tcacgacttt ttggcggttt taatccagct
     9541 ggtagtaata ctagatcatc tgttccgcca cctaaatcac aaaaaactac gtcataattt
     9601 ttaaattgtt cggcatagtc tttatcttct aggtcaaagt ttttctttat tgcccatctt
     9661 gctacttcag attcgatcct gcacgctgca tcttccactt ttatagtaag ctctttttct
     9721 aatcctaatg tcagcacctt tacttggtga gtgcccaaaa atttagatgc catccttttc
     9781 tgcatttcac tgaacttatc caatttttta agaagccaaa ttggtagcat tgtttgaaag
     9841 tattcaatag taacttcatt atcttcacgt ttgcctttta gcgcttggta ataagcagtt
     9901 gcagctaaaa atgttacgta tggaatatgt gactctactt tattatgtaa cttcttgatg
     9961 tgttggttgc ctaacacttc tggttcagca agttcaccaa ctagaaaata tctctctgtt
    10021 tcatcaataa ctgtagaaac taataaccgg tcctttaaat ctgccggatc ttcaacgata
    10081 ctcgtaaatt ttccttcagc agcttcttta gatatctcta ctacatttgt aggcaattca
    10141 aaaaaataac catcaattaa attcatgtac atactgtttc caaagtccac gttcatacga
    10201 gaaatattca tatttttatt ctctcctttg atttcacttt cctcaatact agcatcttat
    10261 atcactaaag tcaataaaat gaaatcacat ttaaaatcag tgaatacagg ctatttgagg
    10321 attatataat aaaaatgaaa tcagtgaaat cagtagagaa aagtgatttc aggcccgggg
    10381 ctttacgtca aaaaaaaaat cgtgtaagat aaagtcaagt taagtcaatg tgaaagtttt
    10441 ggcattgaat tctggttcta gttattctaa ataaacaaca aaaaaccctg ttgtttgcag
    10501 acaacaaggt taatagattt gatgatacat attttgtttg cgagctattt gcaaaattga
    10561 atacaaataa agaatgacaa ataaaaagtg tccgatgttg cagcatcgaa cacttggttt
    10621 gtaagccctc acttacaatg tttaagttat gtcttcattt taccgaaaac aagtgtatac
    10681 gtcaacactt ttcggctatt tttggacgac agaaaaccac tgtttggagg gtatcaaacc
    10741 gtggttttct gtcgtttttt gtttgtcacc cagctgccgt taaggggtgt aaaataaact
    10801 gacgtcagga gttgccccgt ccaccgcaaa cccggggata taccacaata agcgttcctt
    10861 gctgcgcttg tggtgaatgg cgaggacggc cattaacggt ccggagaatg tggataaacc
    10921 actaggttgc ttggcgcact acggtgcggg caactatggc caagcttcta tgcagcggca
    10981 cacaattgag tgtgaccgtg tgagagaaca cgagacaaac cccatagaag ttgcatacag
    11041 gacaagcatt gtatgtaaaa aggtcatggc aggcgaaagc ctggcgatag caaggcagag
    11101 ctggaaacct cagacgtctc tatgacgcta gtttcaaatc ctgatcgatt gcctatgtct
    11161 gtccgttttt attttttgcg cgacagacat aggcaatcga aattcgccct gtttcctaga
    11221 acagtcagct attctgctag tttcaagttc tcaaagtcaa gagaaaaaca ctaaataaat
    11281 gcgaatttcc ttaaaaccca gaggttttaa gttccttttt aagggttgtt cgtttgatag
    11341 aacattagta ttttaatata tttttcctgt gggatttcac taacgtatac tgttattccc
    11401 tttggcctat cttaaaaact tgtatagatt gtgtttgagc cgtttagtat gatatcagta
    11461 catgcttggg ttcagagcat caaaatgaga tttgaacatt aagcactatg tactaaatgt
    11521 tctgtgtttt aaaatgtttg atgatgccga taagtattag gactagtttt gtttttgaat
    11581 agctggaggg aagtgtatgt tgtccaaagt aaaaaaagta ccgtctccct atgtaggcaa
    11641 tctgttgaat aaatggcatg actatattat gcaggagaag gttcatgagt ctatagagaa
    11701 aagaactgaa ataaagcaat tgttaagtca ggctgaagat aataaggacc tggttgatta
    11761 ttttattcta ctggatcatc gtcatagcct ttgttttgat caagaggcat ctatgggtga
    11821 tgtcgttaat atgttaagta agggaagtca tgatctttta ataaattttt attttgaatt
    11881 gtttgcaggg gattatgagt tttttaaaaa aaattatgtt aaagcaattt ctttttatga
    11941 aaaagctgaa caaaagttat cgagtattcc caatattgag gaaacgaagt ttgcggagtt
    12001 tcactataag attggaggct aatgacctag ggcgttcggc tgcggcgagc ggtatcagct
    12061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    12601 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    12661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    12721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    12781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    12841 actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
    12901 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctctc
    12961 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
    13021 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
    13081 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
    13141 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
    13201 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
    13261 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
    13321 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
    13381 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
    13441 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
    13501 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
    13561 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
    13621 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
    13681 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
    13741 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
    13801 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
    13861 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga cgtctaagaa
    13921 accattatta
//
