LOCUS       sequence-467187-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-stranded recombineering in Lactococcus lactis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Filsinger GT, Mychack A, Lyerly E, Henriksen C, Bartlett TM, 
            Kuchwara H, Eitzinger S, Bernhardt TG, Walker S, Church GM, Wannier 
            TM
  TITLE     A diverse single-stranded DNA-annealing protein library enables 
            efficient genome editing across bacterial phyla.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Apr 29;122(17):e2414342122. doi: 
            10.1073/pnas.2414342122. Epub 2025 Apr 21.
  PUBMED    40258142
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-04-29"; volume: "122(17):e2414342122. 
            doi"; pages: " 10.1073/pnas.2414342122. Epub 2025 Apr 21"
COMMENT     Sequence Label: pORTMAGE-Ll1
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1473..1484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gatctagtct tataactata ctgacaatag aaacattaac aaatctaaaa cagtcttaat
       61 tctatcttga gaaagtattg gtaataatat tattgtcgat aacgcgagca taataaacgg
      121 ctctgattaa attctgaagt ttgttagata caatgatttc gttcgaagga actacaaaat
      181 aaattataac acaaaaggag gtttttttat ggctgaaaat gaaaaacaag ctcttcttca
      241 agaagaaaac aaatctgaaa acgtcgtttc aactgttaaa cgtactgcac ttgctacaaa
      301 ccctttttca gatactgacc aatttaataa catttttaaa atggctcaat taatttcaca
      361 atctgatatg attccagcta catataaagg aaaacctatg aattgtgtaa tcgctcttga
      421 acaagctaat cgtatgggag tctctccact tatggttatg caaaatcttt atgtcgtcaa
      481 aggagttcct tcatggagtg gacaaggttg tatgatgatt atccaaggtt gtggtaaatt
      541 tcgtgatgtt gattacgttt actctggtga aaaaggaaca gatagtcgta gttgtaaagt
      601 tgttgctaca cgtatttctg atggaaaacg tatcgaagga actgaaatca caatgcaaat
      661 ggtcaaatct gaaggttgga ttagtaatac aaaatggaaa aatatgccag aacaaatgtt
      721 aggttatcgt gcagccactt tctttgcacg tatgtattgt ccaaatgaac ttaatggttt
      781 tgcaacagaa ggagaagcgg aagatatgaa ccataaacca caacgtattg aagctattaa
      841 tgttttaggt gatacagctc atgaataagg taccactagt tctagagagc tcaagctttc
      901 tttgaaccaa aattagaaaa ccaaggcttg aaacgttcaa ttgaaatggc aattaaacaa
      961 attacagcac gtgttgcttt gattgatagc caaaaagcag cagttgataa agcaattact
     1021 gatattgctg aaaaattgta atttataaat aaaaatcacc ttttagaggt ggttttttta
     1081 tttataaatt attcgtttga tttcgctttc gatagaacaa tcaaagcgag aataaggaag
     1141 ataaatccca taagggcggg agcagaatgt ccgagactaa tgtaaatttg tccaccaatt
     1201 aaaggaccga taacgcgagc ttctcgagtg catattttcg gcaatcttct caatgagatg
     1261 ctcttcagca tgttcaatga tgtcgatttt ttattaaaac gtctcaaaat cgtttctgag
     1321 acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg cgttatcgta
     1381 gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg ttagattatg
     1441 aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt tggaggaggc
     1501 tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa ttgcttgcaa
     1561 ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg
     1621 gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg ccgagtgcca
     1681 atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg tttttcaatc
     1741 gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt aagtagcttt
     1801 atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt tgattggagt
     1861 tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga caaaagagca
     1921 agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg cggttgcggc
     1981 gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag cgaaagctcg
     2041 cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt atatcatggc
     2101 tattaaaaat actaaagtta gaaattttgg atttttatta tatcctgact caattcctaa
     2161 tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc ctttacacga
     2221 tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac gaaatggaaa
     2281 gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg taacaataga
     2341 aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc atgttgagat
     2401 acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg acgctattgc
     2461 taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt ttgatattga
     2521 ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac ttttagatat
     2581 agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc gccttagggg
     2641 agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa caaactctag
     2701 cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa gttatgcaaa
     2761 ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga gttatttgct
     2821 gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga aagatacaga
     2881 gaaatggaag ttgaattaag tacaacaatc taaattcact ttagataaaa atttaggagg
     2941 catatcaaat gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta
     3001 atcattattt gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt
     3061 tataccgaaa cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga
     3121 caagggtgat aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag
     3181 gttattggga taagttagag ccactttata caatttttga tggtgtatct aaaacattct
     3241 ctggtatttg gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg
     3301 atgtagagaa atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg
     3361 ctttttctct ttctattatt ccttggactt catttactgg gtttaactta aatatcaata
     3421 ataatagtaa ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt
     3481 caatatattt accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgctg
     3541 gattgtttat gaactctatt caggaattgt cagataggcc taatgactgg cttttataat
     3601 atgagataat gccgactgta ctttttacag tcggttttct aatgtcacta acctgccccg
     3661 ttagttgaag aaggttttta tattacagct cca
//
