LOCUS       sequence-467188-       11203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrating SSAP (HapRecT) and cutting cas9 (Sth3) into Caulobacter 
            crescentus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Filsinger GT, Mychack A, Lyerly E, Henriksen C, Bartlett TM, 
            Kuchwara H, Eitzinger S, Bernhardt TG, Walker S, Church GM, Wannier 
            TM
  TITLE     A diverse single-stranded DNA-annealing protein library enables 
            efficient genome editing across bacterial phyla.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Apr 29;122(17):e2414342122. doi: 
            10.1073/pnas.2414342122. Epub 2025 Apr 21.
  PUBMED    40258142
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-04-29"; volume: "122(17):e2414342122. 
            doi"; pages: " 10.1073/pnas.2414342122. Epub 2025 Apr 21"
COMMENT     Sequence Label: pORTMAGE-Cc1
FEATURES             Location/Qualifiers
     source          1..11203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..105
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      106..152
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(7734..7750)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7761..7777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7975..8563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8055..8074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8669..8685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(8782..9981)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             10087..10737
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     oriT            11031..11140
                     /note="incP origin of transfer"
ORIGIN
        1 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
       61 gcagcggaaa agatccgtcg acggccgctc tagaactagt ggatccaaat aaaacgaaag
      121 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta tacgcaaggc
      181 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
      241 gtcggcagaa tgcttaatga attacaacag tttttatata agcttagcgc taccggactc
      301 agatccagat cgaaatgtac cggctgaact ggtggaagca cgttgagcct cgtcaaggac
      361 tcatgaagcg cgtccctgct ctcagtcgcc tgctcgttac gcctgaggtg tcaaaacacc
      421 gtctgttcat ttggctagac gctagagttc ttccggatca caagctccag gtcgttacac
      481 tcgatgacga ctgctcattc ggagttttac actcaaggtt ccacgaggtg tgggctcttg
      541 cagcggggtc gtggcacggc tcaggcaatg atccgcgcta caccatctcc accaccttcg
      601 agaccttccc cttcccggaa ggcctgacgc ccaacatagc cgccgtcgac tatgagggcg
      661 acccgcgcgc ccaggccatc gccgcggcgg cggccgagct gaaccggctg cgggaagcct
      721 ggctcaatcc gcccgacctg gtgcggatcg agcccgaggt cgtgcccggc tatcccgacc
      781 gcgtgctgcc cgtcagcccc gaggcgggcg ccgagctgaa gaagcgcacc ctgaccaacc
      841 tctacaacca gcgcccggcc tggctggaca tggcccacca gcgcctggac gccgccgtcg
      901 ccgccgccta cggctggccc gacggcctga ccgacgacga gatcctggag cgcctgttcg
      961 ccctcaacca ggagcgcgcg gcggcggggc ggtaggttag acccctccaa aaccccgacc
     1021 tccccggcga aggccggggt ccagttacat ccggaacgct tggagtgggc ccgcctacgg
     1081 cctcgaccaa cccgcagtcg gcaaggggtt cgatctggac cccggcattc gccggggagg
     1141 tcggcagaga ggtcagaacc gcgtcttgca gccctgcttc agatgaaccc cgcgcaggaa
     1201 gccgcggcgg gccaggccgg cgtcgacggc ggcgttcatc tggcggtcgg cgggggcagc
     1261 gccggtgact tcgcgcacca ggccccggaa cgggatgatg ctgttgatca gggtcttgcc
     1321 accggcctcg gccagcttgc cggctcggtt ttcggcgtac tcggcgccga ccttgtagtc
     1381 gggccccagc gccgcgttca gcgccttcac ctcattggcc agggcctggc aggacttcag
     1441 acccgcgagg ctgtaggggt cgtcaaaggc cttggccagg accggcggca cctcgcgctt
     1501 ggacaggccg acgtcgcgca ccggctgggt gccgatgtcg acggccttct tgcccgcctc
     1561 gccccagcgg ccggacggtt cctcgggctt ggccggcggc ttgacctggg cgtgggcgct
     1621 ggaggccagc agcacggcgg cggctccggc catgatcagc agcttgttcg gcatcaaaag
     1681 cgccctcccg acaaaacacg ccacgggaac ggcaagcctc gccgccggtt cccgcccgca
     1741 agtccctcgg cacgaactct aaggcctgcg cacgatccat cgccaccaca aagcgtgtca
     1801 tcccggccga agcgcagcgg agagccggga cccagggcca caagcacggc gcttcgtcac
     1861 cccctgggtc ccggatagcg ctccgcgctt ccgggatgac acgggtttaa gcacttgggt
     1921 gagaagccct agttcggcgc gccgcccgct tccaggtcga acgcggcgtc gaaggcgtcg
     1981 tcgccaggaa ccgtcccttc cagggcggcg tcgaacaggt cttcgagcga tgaccagatg
     2041 aaggccatct gctccatgat cgtcgggacg ctggccggat cgaagccggg gtagaggtct
     2101 tccaccagcc acaggcccgt gccgggatcg aaggcgaagc ggcagccgat caggcggaac
     2161 tgggcgcggg ccatggtctc gaacagggcc gtcaggtcgc gggcggcgtc caggtcgtcg
     2221 tggtccagca ccaggcagcg gatctgcagc cagccgtggt cgggcagcag gtagaaggcg
     2281 ccctcgtcct gatcctcgcc cgaaacctcc agcccccggt cgatggcttc gacgacatag
     2341 ccggccgcgc ggcaggtgtc ggtgagcgcg gccagcaggg cggcttcctg gtcaggggtc
     2401 aggtcggtca tgggcaagag gtccaggtcg tggtttgtcg gcggcttcta gcatggaccg
     2461 cccgcgcccg tgaggccgag gatttcgcgc tggtcagaca acctacttgc cgtccccaca
     2521 tgttagcgct accaagtgcc gacgaacgcg cgccgccgac ggtgtcggcg cttcagacgc
     2581 tcgagtttta aaaggaggtt ttttatgtcg aatacgatga tgctcgcccc ccagacgttt
     2641 gaccaagcga tgcagttcgc gaatgccatc gccgcctccc aatttgcgcc ctcctcctac
     2701 cgcggcaagc cgaacgatgt cctgatcgcg atgcagatgg gcgccgagct cgggttccag
     2761 cccatgcagt cggtgcaagg gatcgccgtc atcaatggcc gtccgtcggt gtggggggat
     2821 gcgctccgtg ccctgatcct gtccgcgccg gacctggccg aattcgaaga gtcctacgac
     2881 gaagccacgc aaacggccca ttgtaagatc tcgcggcgtc tccagaccgg gtcgatcgcg
     2941 acgttcaact cctcgttctc cgtgacggat gcgcagacgg cggggctgtg gggcaagaac
     3001 ggcccctgga agcagtatcc gaagcggatg caacaatggc gtgcgctcgg cttctgtgcg
     3061 cgggactcgt atgccgaccg tctcaagggg atccaactcg cggaagaagt gcaagattat
     3121 gaaccgatcg agaaggtcgt ccataccccg agccaagacc aagattccgc catcgagaat
     3181 aagatcacgg aggaacagtc gaaccgcatc aatgagatcc tgatctcggt cgatagcacc
     3241 ttcgacgatc tgaagaaggc ctgtaagtcc atgaccgggc gtgatatcga caatcagtcg
     3301 gaactcacga gcacggaggc cgccaagctg atctcgtccc tggaacggaa gctggcgagc
     3361 aagaccggcg gggaaaagga tgccgcctaa agctcctgac tcaggaggta tcgatatgac
     3421 caagccgtac tcgatcggcc tggacatcgg caccaactcg gtgggctggg ccgtgaccac
     3481 cgacaactac aaggtgccgt cgaagaagat gaaggtgctg ggcaacacct cgaagaagta
     3541 catcaagaag aacctgctgg gcgtgctgct gttcgactcg ggcatcaccg ccgagggccg
     3601 ccgcctgaag cgcaccgccc gccgccgcta cacccgccgc cgcaaccgca tcctgtacct
     3661 gcaggagatc ttctcgaccg agatggccac cctggacgac gccttcttcc agcgcctgga
     3721 cgactcgttc ctggtgccgg acgacaagcg cgactcgaag tacccgatct tcggcaacct
     3781 ggtggaggag aaggcctacc acgacgagtt cccgaccatc taccacctgc gcaagtacct
     3841 ggccgactcg accaagaagg ccgacctgcg cctggtgtac ctggccctgg cccacatgat
     3901 caagtaccgc ggccacttcc tgatcgaggg cgagttcaac tcgaagaaca acgacatcca
     3961 gaagaacttc caggacttcc tggacaccta caacgccatc ttcgagtcgg acctgtcgct
     4021 ggagaactcg aagcagctgg aggagatcgt gaaggacaag atctcgaagc tggagaagaa
     4081 ggaccgcatc ctgaagctgt tcccgggcga gaagaactcg ggcatcttct cggagttcct
     4141 gaagctgatc gtgggcaacc aggccgactt ccgcaagtgc ttcaacctgg acgagaaggc
     4201 ctcgctgcac ttctcgaagg agtcgtacga cgaggacctg gagaccctgc tgggctacat
     4261 cggcgacgac tactcggacg tgttcctgaa ggccaagaag ctgtacgacg ccatcctgct
     4321 gtcgggcttc ctgaccgtga ccgacaacga gaccgaggcc ccgctgtcgt cggccatgat
     4381 caagcgctac aacgagcaca aggaggacct ggccctgctg aaggagtaca tccgcaacat
     4441 ctcgctgaag acctacaacg aggtgttcaa ggacgacacc aagaacggct acgccggcta
     4501 catcgacggc aagaccaacc aggaggactt ctacgtgtac ctgaagaagc tgctggccga
     4561 gttcgagggc gccgactact tcctggagaa gatcgaccgc gaggacttcc tgcgcaagca
     4621 gcgcaccttc gacaacggct cgatcccgta ccagatccac ctgcaggaga tgcgcgccat
     4681 cctggacaag caggccaagt tctacccgtt cctggccaag aacaaggagc gcatcgagaa
     4741 gatcctgacc ttccgcatcc cgtactacgt gggcccgctg gcccgcggca actcggactt
     4801 cgcctggtcg atccgcaagc gcaacgagaa gatcaccccg tggaacttcg aggacgtgat
     4861 cgacaaggag tcgtcggccg aggccttcat caaccgcatg acctcgttcg acctgtacct
     4921 gccggaggag aaggtgctgc cgaagcactc gctgctgtac gagaccttca acgtgtacaa
     4981 cgagctgacc aaggtgcgct tcatcgccga gtcgatgcgc gactaccagt tcctggactc
     5041 gaagcagaag aaggacatcg tgcgcctgta cttcaaggac aagcgcaagg tgaccgacaa
     5101 ggacatcatc gagtacctgc acgccatcta cggctacgac ggcatcgagc tgaagggcat
     5161 cgagaagcag ttcaactcgt cgctgtcgac ctaccacgac ctgctgaaca tcatcaacga
     5221 caaggagttc ctggacgact cgtcgaacga ggccatcatc gaggagatca tccacaccct
     5281 gaccatcttc gaggaccgcg agatgatcaa gcagcgcctg tcgaagttcg agaacatctt
     5341 cgacaagtcg gtgctgaaga agctgtcgcg ccgccactac accggctggg gcaagctgtc
     5401 ggccaagctg atcaacggca tccgcgacga gaagtcgggc aacaccatcc tggactacct
     5461 gatcgacgac ggcatctcga accgcaactt catgcagctg atccacgacg acgccctgtc
     5521 gttcaagaag aagatccaga aggcccagat catcggcgac gaggacaagg gcaacatcaa
     5581 ggaggtggtg aagtcgctgc cgggctcgcc ggccatcaag aagggcatcc tgcagtcgat
     5641 caagatcgtg gacgagctgg tgaaggtgat gggcggccgc aagccggagt cgatcgtggt
     5701 ggagatggcc cgcgagaacc agtacaccaa ccagggcaag tcgaactcgc agcagcgcct
     5761 gaagcgcctg gagaagtcgc tgaaggagct gggctcgaag atcctgaagg agaacatccc
     5821 ggccaagctg tcgaagatcg acaacaacgc cctgcagaac gaccgcctgt acctgtacta
     5881 cctgcagaac ggcaaggaca tgtacaccgg cgacgacctg gacatcgacc gcctgtcgaa
     5941 ctacgacatc gaccacatca tcccgcaggc cttcctgaag gacaactcga tcgacaacaa
     6001 ggtgctggtg tcgtcggcct cgaaccgcgg caagtcggac gacgtgccgt cgctggaggt
     6061 ggtgaagaag cgcaagacct tctggtacca gctgctgaag tcgaagctga tctcgcagcg
     6121 caagttcgac aacctgacca aggccgagcg cggcggcctg tcgccggagg acaaggccgg
     6181 cttcatccag cgccagctgg tggagacccg ccagatcacc aagcacgtgg cccgcctgct
     6241 ggacgagaag ttcaacaaca agaaggacga gaacaaccgc gccgtgcgca ccgtgaagat
     6301 catcaccctg aagtcgaccc tggtgtcgca gttccgcaag gacttcgagc tgtacaaggt
     6361 gcgcgagatc aacgacttcc accacgccca cgacgcctac ctgaacgccg tggtggcctc
     6421 ggccctgctg aagaagtacc cgaagctgga gccggagttc gtgtacggcg actacccgaa
     6481 gtacaactcg ttccgcgagc gcaagtcggc caccgagaag gtgtacttct actcgaacat
     6541 catgaacatc ttcaagaagt cgatctcgct ggccgacggc cgcgtgatcg agcgcccgct
     6601 gatcgaggtg aacgaggaga ccggcgagtc ggtgtggaac aaggagtcgg acctggccac
     6661 cgtgcgccgc gtgctgtcgt acccgcaggt gaacgtggtg aagaaggtgg aggagcagaa
     6721 ccacggcctg gaccgcggca agccgaaggg cctgttcaac gccaacctgt cgtcgaagcc
     6781 gaagccgaac tcgaacgaga acctggtggg cgccaaggag tacctggacc cgaagaagta
     6841 cggcggctac gccggcatct cgaactcgtt caccgtgctg gtgaagggca ccatcgagaa
     6901 gggcgccaag aagaagatca ccaacgtgct ggagttccag ggcatctcga tcctggaccg
     6961 catcaactac cgcaaggaca agctgaactt cctgctggag aagggctaca aggacatcga
     7021 gctgatcatc gagctgccga agtactcgct gttcgagctg tcggacggct cgcgccgcat
     7081 gctggcctcg atcctgtcga ccaacaacaa gcgcggcgag atccacaagg gcaaccagat
     7141 cttcctgtcg cagaagttcg tgaagctgct gtaccacgcc aagcgcatct cgaacaccat
     7201 caacgagaac caccgcaagt acgtggagaa ccacaagaag gagttcgagg agctgttcta
     7261 ctacatcctg gagttcaacg agaactacgt gggcgccaag aagaacggca agctgctgaa
     7321 ctcggccttc cagtcgtggc agaaccactc gatcgacgag ctgtgctcgt cgttcatcgg
     7381 cccgaccggc tcggagcgca agggcctgtt cgagctgacc tcgcgcggct cggccgccga
     7441 cttcgagttc ctgggcgtga agatcccgcg ctaccgcgac tacaccccgt cgtcgctgct
     7501 gaaggacgcc accctgatcc accagtcggt gaccggcctg tacgagaccc gcatcgacct
     7561 ggccaagctg ggcgagggct aaatactctg gccatttttc tttaccgcct ttgagtgagc
     7621 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgct gattagctaa
     7681 gcagaaggcc atcctgacgg atgccttttt cgcttgctag ctgcagcccg ggggatccac
     7741 tagttctaga gcggccattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     7801 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagcgg tatcagctca
     7861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8401 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatacg
     8641 aggccctttc gtcttcaaga attaattcac tggccgtcgt tttacaacgt cgtgactggg
     8701 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagcagat
     8761 ccgtccctat cgtttccacg atcagcgatc ggctcgttgc cctgcgccgc tccaaagccc
     8821 gcgacgcagc gccggcaggc agagcaagta gagggcagcg cctgcaatcc atgcccaccc
     8881 gttccacgtt gttatagaag ccgcatagat cgccgtgaag aggaggggtc cgacgatcga
     8941 ggtcaggctg gtgagcgccg ccagtgagcc ttgcagctgc ccctgacgtt cctcatccac
     9001 ctgcctggac aacattgctt gcagcgccgg cattccgatg ccacccgaag caagcaggac
     9061 catgatcggg aacgccatcc atccccgtgt cgcgaaggca agcaggatgt agcctgtgcc
     9121 gtcggcaatc attccgagca tgagtgcccg cctttcgccg agccgggcgg ctacagggcc
     9181 ggtgatcatt gcctgggcga gtgaatgcag aatgccaaat gcggcaagcg aaatgccgat
     9241 cgtggtcgcg tcccagtgaa agcgatcctc gccgaaaatg acccaaagcg cggccggcac
     9301 ctgtccgaca agttgcatga tgaagaagac cgccatcagg gcggcgacga cggtcatgcc
     9361 ccgggcccac cggaacgaag cgagcgggtt gagagcctcc cggcgtaacg gccggcgttc
     9421 gcctttgtgc gactccggca aaaggaaaca gcccgtcagg aaattgaggc cgttcaaggc
     9481 tgccgcggcg aagaacggag cgtgggggga gaaaccgccc atcagcccac cgagcacagg
     9541 tcccgcgacc atcccgaacc cgaaacaggc gctcatgaag ccgaagtgcc gcgcgcgctc
     9601 atcgccatca gtgatatcgg caatataagc gccggctacc gccccagtcg ccccggtgat
     9661 gccggccacg atccgcccga tatagagaac ccaaaggaaa ggcgctgtcg ccatgatggc
     9721 gtagtcgaca gtggcgccgg ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc
     9781 tgacagcgcg cccagcacag gtgcgcaggc aaattgcacc aacgcataca gcgccagcag
     9841 aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag
     9901 caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat
     9961 caggggtatg ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac
    10021 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg
    10081 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac
    10141 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag
    10201 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc
    10261 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt
    10321 ctgatcggga atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc
    10381 atccatgccg gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc
    10441 ttcctctgcg aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc
    10501 tacttcactg ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc
    10561 ggcggcaccg ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc
    10621 gacgaagccg gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg
    10681 gcgaaaagga ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca
    10741 ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc
    10801 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
    10861 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcctgct
    10921 gcggcgtccg ctcgtatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
    10981 gagacacaac gtggctttcc cccccccccc ccaccgcggt ggcggccgcc ccggccagcc
    11041 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
    11101 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
    11161 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tat
//
