LOCUS       sequence-467227-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the TEF3 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM25
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(731..765)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(836..1495)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1410..1429
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1677..2265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2166..2185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgaacaccg atgaagcaaa gaagtaacag caggaaagaa aaacaaacac
       61 aacaaaaaaa aacaagcagc atagcatcaa cagaaatttc taaagagaac caaattcacc
      121 ccagaaacaa ccgcacaaat acgacatcca tccacctttc ttttatcttc tttttctgat
      181 ctgataatta gtttcatata caatacgtag aaacaggcgc acagcaccca gacctggctt
      241 ctgccccagt gtataagcaa tgtagcataa ttggaaaaaa aaacgaaaaa taccgaaaat
      301 aagtgggaag ctgggccaca ggagtggggc gggatgcgac tggttctgag cgggaccggg
      361 taataaggtt gaaaaacttt gaattgatgg aataagtaac ttctttcttt tcgctggcgg
      421 gagaaggaaa aaaaaaaatt ttttttcctt ctgtttagta ctgggacatt gagaaggcgt
      481 gtcaattttg aataattaga gtggtcaaaa aaattttttt tgcttgggat accctttttc
      541 gataatgtaa attttttttg aatataaaag gagattgaaa aattttttct agcagaaatg
      601 ttttcaagtt ttaattgcaa gtttcgtttg agtattcagt tgtattttag ttgatttgta
      661 gtttatttac tagtattctc atagttctaa ctccaagaga agtaacatta aagtatgtga
      721 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
      781 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      841 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      901 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      961 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     1021 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     1081 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     1141 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     1201 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     1261 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     1321 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     1381 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1441 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1501 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1561 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     1621 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1681 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1741 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1801 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1861 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1921 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1981 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2041 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2101 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2221 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2281 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2341 tcccctgatt ctgtggataa ccgtag
//
