LOCUS       sequence-467233-        2356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the INU1 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM22
FEATURES             Location/Qualifiers
     source          1..2356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(721..755)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(826..1485)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1400..1419
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1667..2255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2156..2175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgataccac ccgccatgga aaaaaatgat gttgatgttg agttagttgg
       61 gttgagtcaa ttagtgcgtg aaagtatcac cacttttgtc atccggcgtt tctgtgcgaa
      121 tcacacacac acacacacac agtttattgg agcgcttgtt tctggcgtat tcgtaattgt
      181 tctgcggtgc ggttctgtgt gcatttttcc tggggtgtct gccgcaccta ctcatcaccc
      241 acgccgtggg tttgagccat ggcggaggta cgactgactg gctgcctgcc tgcctgactg
      301 actgcctgac tgcaggaaaa gagggtttcg aaggaaaaac ttttcctgtg ttaatccggc
      361 cgtgcgccgc tgctccaaaa tccatcttca tgagaaggag tttgaaaaaa caaaaaaatt
      421 cacatataaa aagcgtatct cgagatctca aagtctccct tgaatcgtgt ttgccagttg
      481 taactcatcc tttattcttc tattctatct ctctctttcc ttcccctaat cagcaattaa
      541 atccggggta aggaagaatt actactgtgt gtaacggtta tatttcgttt tttatttttt
      601 tttccattgc catagagaaa gaaaaaaaaa aaaaagagag tttgtgaaga tcttccattc
      661 gaatcccata agtgacacat ttaatttttt ttttgttaga tcctatgtga gaccagacca
      721 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
      781 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
      841 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      901 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      961 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1021 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     1081 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1141 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1201 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1261 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1321 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1381 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1441 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1501 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1561 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     1621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1801 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2341 ctgtggataa ccgtag
//
