LOCUS       sequence-467234-       19069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19069)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 19069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHUE411
FEATURES             Location/Qualifiers
     source          1..19069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..380
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             660..1451
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1603..1678
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1982..3974
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3993..4058
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4065..4085
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4110..8210
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4110..8210
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8123..8131
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8211..8258
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(8915..8931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8915..8931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8928..8950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8939..8955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8963..8969,8970..8987,8988..8993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9008..9029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9220..9896
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9833..9853
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9963..10988
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    11029..11203
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11281..11305
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11730..12524
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13810..14398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14299..14318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14552..14569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14584..14724
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14650..14669)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14810..14832
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15068..15262
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15328..16401)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16830..17459)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    18760..18784
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     18972..18994
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18986..19003
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     18987..19003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaggaatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa cttaaagtta
       61 tcaggcatgc atggatcttg gaggaatcag atgtgcagtc agggaccata gcacaagaca
      121 ggcgtcttct actggtgcta ccagcaaatg ctggaagccg ggaacactgg gtacgttgga
      181 aaccacgtga tgtgaagaag taagataaac tgtaggagaa aagcatttcg tagtgggcca
      241 tgaagccttt caggacatgt attgcagtat gggccggccc attacgcaat tggacgacaa
      301 caaagactag tattagtacc acctcggcta tccacataga tcaaagctga tttaaaagag
      361 ttgtgcagat gatccgtggc gtgagaccaa cccagtggac ataagcctgt tcggttcgta
      421 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
      481 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
      541 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
      601 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
      661 tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      721 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
      781 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
      841 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
      901 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
      961 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1021 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1081 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1141 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1201 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1261 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1321 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1381 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     1441 tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt
     1501 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
     1561 ctgcttttat tatttttaag cgtgcataat aagccggtct cggttttaga gctagaaata
     1621 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     1681 ttttttttcg ttttgcattg agttttctcc gtcgcatgtt tgcagtttta ttttccgttt
     1741 tgcattgaaa tttctccgtc tcatgtttgc agcgtgttca aaaagtacgc agctgtattt
     1801 cacttattta cggcgccaca ttttcatgcc gtttgtgcca actatcccga gctagtgaat
     1861 acagcttggc ttcacacaac actggtgacc cgctgacctg ctcgtacctc gtaccgtcgt
     1921 acggcacagc atttggaatt aaagggtgtg atcgatactg cttgctgcta agcttgcatg
     1981 cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
     2041 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
     2101 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
     2161 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
     2221 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
     2281 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
     2341 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
     2401 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
     2461 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
     2521 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
     2581 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
     2641 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     2701 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     2761 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     2821 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     2881 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     2941 acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc ccctctctac
     3001 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
     3061 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
     3121 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
     3181 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
     3241 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
     3301 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
     3361 ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt
     3421 atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc
     3481 taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt
     3541 tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt
     3601 agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca
     3661 tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca
     3721 tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg
     3781 ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc
     3841 ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct
     3901 tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg
     3961 tgttacttct gcagccctag gcctactaga tggattacaa ggaccacgac ggggattaca
     4021 aggaccacga cattgattac aaggatgatg atgacaagat ggctccgaag aagaagagga
     4081 aggttggcat ccacggggtg ccagctgctg acaagaagta ctcgatcggc ctcgatattg
     4141 ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt
     4201 tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc
     4261 tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt
     4321 acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga
     4381 aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga
     4441 agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt
     4501 accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc
     4561 gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg
     4621 gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt
     4681 acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc
     4741 tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg
     4801 agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact
     4861 tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg
     4921 acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg
     4981 ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga
     5041 ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc
     5101 tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct
     5161 tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt
     5221 tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga
     5281 agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac
     5341 accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt
     5401 tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg
     5461 tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga
     5521 cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca
     5581 tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact
     5641 cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg
     5701 agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc
     5761 tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga
     5821 ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg
     5881 ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga
     5941 atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga
     6001 tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca
     6061 agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg
     6121 acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca
     6181 atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc
     6241 aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag
     6301 ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg
     6361 gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga
     6421 agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg
     6481 ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct
     6541 acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca
     6601 ggctcagcga ttacgacgtc gatcatatcg ttccacagtc attcctgaag gatgactcca
     6661 ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt
     6721 cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga
     6781 tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg
     6841 acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg
     6901 cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg
     6961 aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt
     7021 tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg
     7081 tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg
     7141 actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg
     7201 ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc
     7261 tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga
     7321 tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag
     7381 tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattctgc
     7441 ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg
     7501 gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg
     7561 gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct
     7621 ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga
     7681 aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc
     7741 ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg ccgtccaagt
     7801 atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca
     7861 acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc
     7921 agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct
     7981 ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc
     8041 tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac acaactatcg
     8101 atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc caccagtcga
     8161 ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac aagcggccag
     8221 cggcgacgaa gaaggcgggg caggcgaaga agaagaagtg agctcagagc tttcgttcgt
     8281 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc gcacacacca
     8341 gaatcctact gagtttgagt attatggcat tgggaaaact gtttttcttg taccatttgt
     8401 tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa
     8461 tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat
     8521 ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt
     8581 ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag
     8641 taaaacactt gtagttgtac cattatgctt attcactagg caacaaatat attttcagac
     8701 ctagaaaagc tgcaaatgtt actgaataca agtatgtcct cttgtgtttt agacatttat
     8761 gaactttcct ttatgtaatt ttccagaatc cttgtcagat tctaatcatt gctttataat
     8821 tatagttata ctcatggatt tgtagttgag tatgaaaata ttttttaatg cattttatga
     8881 cttgccaatt gattgacaac gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     8941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9121 tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     9181 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     9241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     9301 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     9361 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     9421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg
     9481 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     9541 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     9601 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     9661 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     9721 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     9781 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     9841 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaaatc
     9901 accagtctct ctctacaaat ctatctctct cgagctttcg cagatccggg gggcaatgag
     9961 atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
    10021 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
    10081 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
    10141 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
    10201 ttggggagtt tagcgagagc ctgacctatt gcatctcccg ccgttcacag ggtgtcacgt
    10261 tgcaagacct gcctgaaacc gaactgcccg ctgttctaca accggtcgcg gaggctatgg
    10321 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
    10381 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
    10441 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
    10501 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
    10561 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
    10621 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
    10681 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
    10741 cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
    10801 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
    10861 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
    10921 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
    10981 agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt tctccataat
    11041 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
    11101 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
    11161 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc
    11221 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    11281 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    11341 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    11401 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    11461 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    11521 cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta
    11581 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
    11641 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
    11701 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
    11761 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
    11821 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
    11881 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
    11941 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
    12001 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    12061 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    12121 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    12181 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
    12241 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    12301 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    12361 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
    12421 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    12481 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gggaaggtgc
    12541 gaataagcgg ggaaattctt ctcggctgac tcagtcattt catttcttca tgtttgagcc
    12601 gattttttct cccgtaaatg ccttgaatca gcctatttag accgtttctt cgccatttaa
    12661 ggcgttatcc ccagttttta gtgagatctc tcccactgac gtatcatttg gtccgcccga
    12721 aacaggttgg ccagcgtgaa taacatcgcc agttggttat cgtttttcag caaccccttg
    12781 tatctggctt tcacgaagcc gaactgtcgc ttgatgatgc gaaatgggtg ctccaccctg
    12841 gcccggatgc tggctttcat gtattcgatg ttgatggccg ttttgttctt gcgtggatgc
    12901 tgtttcaagg ttcttacctt gccggggcgc tcggcgatca gccagtccac atccacctcg
    12961 gccagctcct cgcgctgtgg cgccccttgg tagccggcat cggctgagac aaattgctcc
    13021 tctccatgca gcagattacc cagctgattg aggtcatgct cgttggccgc ggtggtgacc
    13081 aggctgtggg tcaggccact cttggcatcg acaccaatgt gggccttcat gccaaagtgc
    13141 cactgattgc ctttcttggt ctgatgcatc tccggatcgc gttgctgctc tttgttcttg
    13201 gtcgagctgg gtgcctcaat gatggtggca tcgaccaagg tgccttgagt catcatgacg
    13261 cctgcttcgg ccagccagcg attgatggtc ttgaacaatt ggcgggccag ttgatgctgc
    13321 tccagcaggt ggcggaaatt catgatggtg gtgcggtccg gcaaggcgct atccagggat
    13381 aaccgggcaa acagacgcat ggaggcgatt tcgtacagag catcttccat cgcgccatcg
    13441 ctcaggttgt accaatgctg catgcagtga atgcgtagca tggtttccag cggataaggt
    13501 cgccggccat taccagcctt ggggtaaaac ggctcgatga cttccaccat gttttgccat
    13561 ggcagaatct gctccatgcg ggacaagaaa atctcttttc tggtctgacg gcgcttactg
    13621 ctgaattcac tgtcggcgaa ggtaagttga tgactcatga tgaaccctgt tctatggctc
    13681 cagatgacaa acatgatctc atatcaggga cttgttcgca ccttccctag aatgcatgac
    13741 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    13801 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    13861 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    13921 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    13981 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    14041 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    14101 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    14161 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    14221 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    14281 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    14341 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    14401 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    14461 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    14521 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    14581 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    14641 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    14701 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    14761 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    14821 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
    14881 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
    14941 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
    15001 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
    15061 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
    15121 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
    15181 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
    15241 cccaatgtac gtgctatcca caggaaacag accttttcga cctttttccc ctgctagggc
    15301 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
    15361 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
    15421 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
    15481 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
    15541 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
    15601 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
    15661 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
    15721 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
    15781 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
    15841 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
    15901 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
    15961 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
    16021 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
    16081 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
    16141 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
    16201 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
    16261 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
    16321 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
    16381 ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag
    16441 ggccggcagg caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
    16501 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
    16561 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
    16621 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
    16681 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
    16741 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
    16801 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    16861 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    16921 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    16981 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    17041 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    17101 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    17161 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    17221 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    17281 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    17341 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    17401 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    17461 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    17521 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    17581 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    17641 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    17701 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    17761 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    17821 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    17881 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    17941 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    18001 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    18061 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    18121 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    18181 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    18241 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    18301 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    18361 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    18421 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    18481 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    18541 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    18601 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
    18661 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
    18721 aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
    18781 gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct
    18841 gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    18901 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    18961 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttagtaattc
    19021 atccaggtct ccaagttcta ggattttcag aactgcaact tattttatc
//
