LOCUS       sequence-467236-        2342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the GLK1A promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM10
FEATURES             Location/Qualifiers
     source          1..2342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(707..741)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(812..1471)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1386..1405
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1653..2241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2142..2161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgccttcac aaaagaaaag tattttgttt ctacacgtat ttctggttat
       61 ggaaggtaac gttatagttt tagtagaagt cagctcggtg gtggtagtgg tggtacctta
      121 ttgatggaca cacgaatgcc cgtaacacta ttgcctacac acagaagctg actagctagt
      181 agacctgact gaagtgccgt tttctagcat atttttcttt ctgggcttag tgtcctgacg
      241 ccccagcgtc agttccttta atttgccagt gtcacagact ggtccttaga tggactacga
      301 gagctttacc cacacataac gtgcttcacc gttactatgt atgctacgta tatacgacaa
      361 tattcctcct gtcttttctt tgcgttcctc tttcacttaa tatgggaacc atttcctatt
      421 ctcaccacac attcgcattc ggattctttt tgctgacgat ctcctgttat tattgcttta
      481 ctcagaaaaa acgacacatt ccctttattg aaataaaatg aaagacggag tctatataaa
      541 gagaagagga gatgagaaca gaccctgttc aggaacagat tatgaaaacg attatggtat
      601 tctttactct cgaactttaa actcgagtga gaatccgctt aaaaatcaag tataccagat
      661 accggaaact agtaacataa aacttaaatt atgtgagacc agaccaataa aaaacgcccg
      721 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
      781 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
      841 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
      901 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
      961 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1021 cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1081 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1141 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1201 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1261 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1321 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1381 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1441 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1501 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1561 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2281 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2341 ag
//
