LOCUS       sequence-467237-        9215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing human MEF2A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Christensen AH, Pan G, Marvig RL, Rodriguez Gonzalez FG, Vissing CR,
            Silajdzija E, Frosted R, Girma EG, Gabrielaite M, Jensen HK, Rossing
            K, Henriksen FL, Sandgaard NCF, Ahlberg G, Ghouse J, Lundegaard PR, 
            Weischenfeldt J, Wadelius C, Bundgaard H
  TITLE     Gain-of-function enhancer variant near KCNB1 causes familial 
            ST-depression syndrome.
  JOURNAL   Eur Heart J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213.
  PUBMED    40208226
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Heart 
            J. 2025 Apr 10:ehaf213. doi: 10.1093/eurheartj/ehaf213."
COMMENT     Sequence Label: pBMN-MEF2A
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     194..211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     195..211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2148..2527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2528..2731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2682..2702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2776..2795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2776..2794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4345..4371
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4381..4446
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4447..4500
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4516..4572
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4594..4659
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4672..5271
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4672..4691)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5168..5188
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5325..5345)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5743..5754)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5972..6186
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     6107..6126
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     LTR             6229..6409
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6481..6602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6518..6537)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6572..6591
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6642..6777
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6810..6826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6810..6826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6823..6845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6834..6850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6858..6864,6865..6882,6883..6888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6903..6924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7041..7058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7212..7800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7971..8762,8763..8831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8594..8613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8832..8936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9004..9022
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9060..9082)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9182..9201
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
       61 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg acgcgtgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg tttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgggatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcggac attgattatt
     2161 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     2221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2701 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     2761 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
     2821 cttaagcttg ccaccatggg gcggaagaaa atacaaatca cacgcataat ggatgaaagg
     2881 aaccgacagg tcacttttac aaagagaaag tttggattaa tgaagaaagc ctatgaactt
     2941 agtgtgctct gtgactgtga aatagcactc atcattttca acagctctaa caaactgttt
     3001 caatatgcta gcactgatat ggacaaagtt cttctcaagt atacagaata taatgaacct
     3061 catgaaagca gaaccaactc ggatattgtt gagactttaa gaaagaaagg ccttaatggt
     3121 tgtgagagcc ctgatgctga cgattacttt gagcacagtc cactctcgga ggacagattc
     3181 agcaaactaa atgaagatag tgattttatt ttcaaacgag gccctcctgg tctgccacct
     3241 cagaactttt caatgtctgt cacagttcca gtgaccagcc ccaatgcttt gtcctacact
     3301 aacccaggga gttcactggt gtccccatct ttggcagcca gctcaacgtt aacagattca
     3361 agcatgctct ctccacctca aaccacatta catagaaatg tgtctcctgg agctcctcag
     3421 agaccaccaa gtactggcaa tgcaggtggg atgttgagca ctacagacct cacagtgcca
     3481 aatggagctg gaagcagtcc agtggggaat ggatttgtaa actcaagagc ttctccaaat
     3541 ttgattggag ctactggtgc aaatagctta ggcaaagtca tgcctacaaa gtctccccct
     3601 ccaccaggtg gtggtaatct tggaatgaac agtaggaaac cagatcttcg agttgtcatc
     3661 cccccttcaa gcaagggcat gatgcctcca ctaaataccc aaaggatcag tagttctcaa
     3721 gccactcaac ctcttgctac cccagtcgtg tctgtgacaa ccccaagctt gcctccgcaa
     3781 ggacttgtgt actcagcaat gccgactgcc tacaacactg attattcact gaccagcgct
     3841 gacctgtcag cccttcaagg cttcaactcg ccaggaatgc tgtcgctggg acaggtgtcg
     3901 gcctggcagc agcaccacct aggacaagca gccctcagct ctcttgttgc tggagggcag
     3961 ttatctcagg gttccaattt atccattaat accaaccaaa acatcagcat caagtccgaa
     4021 ccgatttcac ctcctcggga tcgtatgacc ccatcgggct tcgagcagca gcagcagcag
     4081 cagcagcagc agcagccgcc gccaccaccg cagccccagc cacaaccccc gcagccccag
     4141 ccccgacagg aaatggggcg ctcccctgtg gacagtctga gcagctctag tagctcctat
     4201 gatggcagtg atcgggagga tccacggggc gacttccatt ctccaattgt gcttggccga
     4261 cccccaaaca ctgaggacag agaaagccct tctgtaaagc gaatgaggat ggacgcgtgg
     4321 gtgaccggta ccaagcttgg gccctaccca tacgacgtgc ctgactacgc cgtcaagctc
     4381 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
     4441 gacaaggagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctgggccc
     4501 gctagcggat caggtgccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa
     4561 aaccccggtc ccttaattaa tggctccgga ccggtgaaac agactttgaa ttttgacctt
     4621 ctcaagttgg cgggagacgt ggagtccaac ccaggcccag tcgaccccgg gatgaccgag
     4681 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     4741 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     4801 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4861 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     4921 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     4981 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5041 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5101 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5161 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5221 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg atatcaacct
     5281 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5341 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5401 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5461 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5521 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5581 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5641 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5701 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5761 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc
     5821 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     5881 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5941 gctaattcac tcccaacgat gtcaagaatt ggaacgctga cgtcatcaac ccgctccaag
     6001 gaatcgcggg cccagtgtca ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa
     6061 gatggctgtg agggacaggg gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct
     6121 gggaaatcac cataaacgtg aaatgtcttt ggatttggga atcttataag ttctgtatga
     6181 gaccacttgg atcctctgaa ttcttcgatt ctgctttttg cttctactgg gtctctctgg
     6241 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6301 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6361 aactagagat ccctcagacc ctttcagtca gtgtggaaaa tctctagcag tagtagttca
     6421 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6481 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6541 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6601 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6721 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6781 acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     6841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7021 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7081 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7141 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7201 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7261 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7321 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7381 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7441 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7501 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7561 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7621 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7681 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7741 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7801 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7861 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7921 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7981 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8041 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8101 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8161 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8221 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8281 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8341 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8401 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8461 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8521 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8581 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8641 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8701 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8761 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8821 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8881 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8941 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9001 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     9061 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9121 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     9181 gcggcatcag agcagattgt actgagagtg cacca
//
