LOCUS       sequence-467238-        2365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the RPE1 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM14
FEATURES             Location/Qualifiers
     source          1..2365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(730..764)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(835..1494)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1409..1428
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1676..2264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2165..2184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgtgggcaa cttctcaaac ccatgctcct catcaaattc cgcatcctcg
       61 ctttcctcgt caatgttcaa cacttcaagc ttctcgttta ttctcgacaa cgatggactc
      121 tcattgtact gcgattcatc ttcctgatcc tgctcctgtt gcttcttctt caaatacgaa
      181 tccttcaaca cactcgctgg tgtgttgtga ggagtcggag cattatctat tttgcttgat
      241 ctcgtcgtct taataggcct agacattgct aatttaaaac actctacagc ctcaataccc
      301 tatctacctt ttaaaccggg taccaaaatt accctataat ccaaacacgt ctgtactaag
      361 cacccccaaa gtggtgtgct gttgaactac tctttactaa tgctcaaaaa atttttatgt
      421 acgggttcct atttactacc atatgtatgt tctgaaaata cagtataata ggctaaacta
      481 ttcctaaaat aagacaaaac atatacaaac agacatctcc actcgggagt tccaggaact
      541 agcagcatgt acagacagac acgcacacag agtgtgcaca gctgttatcc aagtaggcaa
      601 tacaacaaat actctgtgta ctaagtacta ctcttgtcat gttttccggt tctatgtatc
      661 tatgcatagt ataatattat tcggcctcgt ggttttccag tttctagctg tgtatgtgag
      721 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
      781 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
      841 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      901 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      961 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1021 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     1081 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1141 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1201 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1261 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1321 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1381 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1441 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1501 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1561 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     1621 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1681 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1741 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1801 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1861 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1921 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1981 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2041 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2101 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2161 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2221 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2281 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2341 cccctgattc tgtggataac cgtag
//
