LOCUS       sequence-467239-        2376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the ZWF promoter for K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM13
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(741..775)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(846..1505)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1420..1439
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1687..2275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2176..2195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcacccgc ctaaaaaaaa agggtgggat tgcatcatat atcacattta
       61 acgacaatgg tggggtggca ccaactactt tgcactcttt aactatggtt tgtcgcttga
      121 taaggtaagg ctccgcatgt agtttaatga ggaatgtggg cggcatcgga atgcaaaact
      181 gtagctacca tttggttgtt aaaatgcttg ccaacaaatc attcaaaaaa aaaaaaaaaa
      241 aaaaaaaaaa aattagatag ctcggttctc catatgttgt gtattataaa tcagttttgc
      301 ttggaacgtt atgacataga aaaatacaaa ttataaaaag agggttctac taaagcgtaa
      361 acgtaatata aaaagtagaa ttgttcattc gttttttgag ttagtggaga ttgataaggt
      421 tttattgcag cagcttagtg tgttctatcc aattgaactt cttacagagc attgttttct
      481 ttcgctttgt actgttttta gtcacgcacc cgcatactcc ttatactcat tggatactca
      541 tttgcttaca gaaagtgtta agaataccct taatcgaata gtaaagaaga acaagcaaaa
      601 gcttctgtcg ctgttctgtt tcctgttact ccaagttttt atctgtgtcg tttcaatttc
      661 acgtcgatat aattgcatat catttctaaa tcgtaacata atactgaaca tttgaacaag
      721 ccctatgtga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      781 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      841 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      901 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      961 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1021 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
     1081 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1141 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1201 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1261 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1321 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1381 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1441 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1501 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1561 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1621 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1681 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1741 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1801 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1861 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1921 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1981 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2041 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2101 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2161 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2221 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2281 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2341 tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
