LOCUS       sequence-467241-        2425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the ALD2 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM16
FEATURES             Location/Qualifiers
     source          1..2425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(790..824)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(895..1554)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1469..1488
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1736..2324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2225..2244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgctctaaa acccatgtcg cattagccgt gtgctaagcg agtcctccgc
       61 cgcttagcgc aaccatcgcc gcttagcaac gagtcgctta tctctctgtg tctgctgcgc
      121 tcggtgttga gtcaagcccg cttcgtatgg gaacggaacg gcatatcttt ccttatccgg
      181 acattctatg cgcaccttag atctatgtta cccggtggac ggacgaaaag agggaaaaaa
      241 aaaaaagaga gagagttgtg tgggtgtatc tctttgtctt aggcggtggg gtcgggatgg
      301 acaaggaatt cagttcccga aataactacc gaaatgcgtt cgggttcttg ctgcgttttg
      361 ctgtgctatg ctgtgctttg ctgcgctgtg cggtcagtgg aaacttcctg ggtccgcgga
      421 tagttaaata acgggggagg cggggggaga gggttgtgtc acgtgatctt gtatcttgaa
      481 aacctagtag gagtggtact ctgcttttct tcagccttta ttggcccagc ccagtgggtt
      541 gggagccttg cggctcccct tctctggtat taacccttct acctgtgact atcaatgact
      601 acctctgggt gctgtttaga taatgaatct gataatgaaa aaaaaaaaaa aggaagcata
      661 caagcttgta taaaagatat atagaaataa agggaagaag aacccgattc ccttgggaat
      721 tataagctgt acggtgggtc agggtccatc agccgtatac aaaagtagaa attatgtgag
      781 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
      841 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
      901 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      961 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1021 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1081 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     1141 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1201 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1261 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1321 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1381 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1441 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1501 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1561 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     1621 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
     1681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2401 cccctgattc tgtggataac cgtag
//
