LOCUS       sequence-467242-        9290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  80X V5 tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9290)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-80XV5
FEATURES             Location/Qualifiers
     source          1..9290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1384..1403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             1637..1678
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1691..1732
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1745..1786
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1799..1840
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1853..1894
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1925..1966
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1979..2020
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2033..2074
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2087..2128
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2141..2182
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2213..2254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2267..2308
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2321..2362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2375..2416
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2429..2470
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2501..2542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2555..2596
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2609..2650
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2663..2704
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2717..2758
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2789..2830
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2843..2884
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2897..2938
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2951..2992
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3005..3046
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3077..3118
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3131..3172
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3185..3226
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3239..3280
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3293..3334
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3365..3406
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3419..3460
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3473..3514
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3527..3568
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3581..3622
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3653..3694
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3707..3748
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3761..3802
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3815..3856
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3869..3910
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3941..3982
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3995..4036
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4049..4090
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4103..4144
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4157..4198
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4229..4270
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4283..4324
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4337..4378
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4391..4432
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4445..4486
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4517..4558
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4571..4612
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4625..4666
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4679..4720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4733..4774
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4805..4846
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4859..4900
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4913..4954
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4967..5008
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5021..5062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5093..5134
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5147..5188
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5201..5242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5255..5296
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5309..5350
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5381..5422
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5435..5476
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5489..5530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5543..5584
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5597..5638
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5669..5710
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5723..5764
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5777..5818
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5831..5872
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5885..5926
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5957..5998
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6011..6052
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6065..6106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6119..6160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6173..6214
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7114..7130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7114..7130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7127..7149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7138..7154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7162..7168,7169..7186,7187..7192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7207..7228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7345..7362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7516..8104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7596..7615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8275..9090)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8275..9090)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8998..9017
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(9091..9195)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9263..9281
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaagtcat acatacacaa
      421 aaggacacgc acccacacag acccatcata gaaaccaatt tgaaattctg aatcctgctt
      481 gacgctcgtt aataaatgcc tggagcagtt tttaatgatt gctgagcttc tgtcactcat
      541 cccgtaaaat gtgtttccgg atttttgcag actgtgttca tgaactggac ggtcgccgtg
      601 gagagccatg tggactcgtc cttcttctgg ggctatctgg tgacgcagat tcccggcggc
      661 ttcatcgcct ccaagttccc ggccaacaag atattcggac tgtccatcgt gagctccgcc
      721 acgctgcatc tcttcgtgcc attcgccatg accctgatgc acggtcatgt ggtgatttgc
      781 gtgagggtcc tgcaaggact cttcgaggta tgtagcccaa ccaacctaac aacttgccca
      841 attcgatttt gccacagatc aaagccgggg attacggctg cgtgatgttg cgaaaatggg
      901 gagggttaga ttttgcttta tgataaagca aatatgtgaa atggaaacat gtcctaagcc
      961 ctcaaaggga tgggggtcct gggagggcag gaaaaaataa caaaagcatt tattttaatg
     1021 cgatttcttt gtgccaagga ctgggaagta tgtcattttt cttcgaatcg gaagggagag
     1081 aatgatactt tgtaaaaagg atgtttgtct attcgagttc cgaagtaact atattttaga
     1141 tatatatttt taatttcaca aagcacattt tcttaagata ctataactgc atgagactaa
     1201 gtgcagagtt tcattaagga ataactaatt ccctaatgaa actcgcgtcg cgccacttca
     1261 acgctcgatg ggagcgtcag cgtcgcgcca cttcaacgct cgatgggagc gtcattggtg
     1321 ggcggggtaa ccgtcgaaat cagtgtttag cccgggcagc atttttttca ctgcattcta
     1381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccca aactcatcaa
     1441 tgtatcttat catgtctgga tcgagtttca ttaaggaata actaattccc taatgaaact
     1501 cttggaatat aggacaatat cctaattgac ttatttaaat aatccattcc tgcaggtgcc
     1561 gtcctacgat ccgcagggat accagcagca gggctccggc ggctggcgcg cccttaagct
     1621 cgagggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     1681 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     1741 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     1801 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     1861 catccccaac cccttactgg gcctggattc caccggctcc ggcggcgtcg agggctccgg
     1921 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     1981 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     2041 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     2101 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     2161 cttactgggc ctggattcca ccggctccgg cggcgtcgag ggctccggcg gcggcaagcc
     2221 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     2281 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     2341 cttactgggc ctggattcca ccggctccgg cggcggcaag cccatcccca accccttact
     2401 gggcctggat tccaccggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     2461 ggattccacc ggctccggcg gcgtcgaggg ctccggcggc ggcaagccca tccccaaccc
     2521 cttactgggc ctggattcca ccggctccgg cggcggcaag cccatcccca accccttact
     2581 gggcctggat tccaccggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     2641 ggattccacc ggctccggcg gcggcaagcc catccccaac cccttactgg gcctggattc
     2701 caccggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     2761 ctccggcggc gtcgagggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     2821 ggattccacc ggctccggcg gcggcaagcc catccccaac cccttactgg gcctggattc
     2881 caccggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     2941 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     3001 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgt
     3061 cgagggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     3121 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     3181 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     3241 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     3301 catccccaac cccttactgg gcctggattc caccggctcc ggcggcgtcg agggctccgg
     3361 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     3421 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     3481 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     3541 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     3601 cttactgggc ctggattcca ccggctccgg cggcgtcgag ggctccggcg gcggcaagcc
     3661 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     3721 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     3781 cttactgggc ctggattcca ccggctccgg cggcggcaag cccatcccca accccttact
     3841 gggcctggat tccaccggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     3901 ggattccacc ggctccggcg gcgtcgaggg ctccggcggc ggcaagccca tccccaaccc
     3961 cttactgggc ctggattcca ccggctccgg cggcggcaag cccatcccca accccttact
     4021 gggcctggat tccaccggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     4081 ggattccacc ggctccggcg gcggcaagcc catccccaac cccttactgg gcctggattc
     4141 caccggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     4201 ctccggcggc gtcgagggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     4261 ggattccacc ggctccggcg gcggcaagcc catccccaac cccttactgg gcctggattc
     4321 caccggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     4381 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     4441 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgt
     4501 cgagggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     4561 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     4621 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     4681 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     4741 catccccaac cccttactgg gcctggattc caccggctcc ggcggcgtcg agggctccgg
     4801 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     4861 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     4921 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     4981 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     5041 cttactgggc ctggattcca ccggctccgg cggcgtcgag ggctccggcg gcggcaagcc
     5101 catccccaac cccttactgg gcctggattc caccggctcc ggcggcggca agcccatccc
     5161 caacccctta ctgggcctgg attccaccgg ctccggcggc ggcaagccca tccccaaccc
     5221 cttactgggc ctggattcca ccggctccgg cggcggcaag cccatcccca accccttact
     5281 gggcctggat tccaccggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     5341 ggattccacc ggctccggcg gcgtcgaggg ctccggcggc ggcaagccca tccccaaccc
     5401 cttactgggc ctggattcca ccggctccgg cggcggcaag cccatcccca accccttact
     5461 gggcctggat tccaccggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     5521 ggattccacc ggctccggcg gcggcaagcc catccccaac cccttactgg gcctggattc
     5581 caccggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     5641 ctccggcggc gtcgagggct ccggcggcgg caagcccatc cccaacccct tactgggcct
     5701 ggattccacc ggctccggcg gcggcaagcc catccccaac cccttactgg gcctggattc
     5761 caccggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     5821 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     5881 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgt
     5941 cgagggctcc ggcggcggca agcccatccc caacccctta ctgggcctgg attccaccgg
     6001 ctccggcggc ggcaagccca tccccaaccc cttactgggc ctggattcca ccggctccgg
     6061 cggcggcaag cccatcccca accccttact gggcctggat tccaccggct ccggcggcgg
     6121 caagcccatc cccaacccct tactgggcct ggattccacc ggctccggcg gcggcaagcc
     6181 catccccaac cccttactgg gcctggattc caccggctcc ggcggcgtcg acggatccgc
     6241 ggccgcataa tgggttcgcg ataggtcact gatatatatc atagctttta gttgtagtcg
     6301 gaaatcgttt gacgtttatt gtttgttctc ggtgtgccgt taaagttttc cccccttttt
     6361 ctctctattt gttgagttcc atcgatggac ggatccgtgg acctttcaga agtatataaa
     6421 tatataacta aagagtaacg aatttgtcag cgaataaggg gattccacat gccacactaa
     6481 cacaatgaaa ctatgtacct aacagagaaa ctacatatgc atacacagca ataccgataa
     6541 ccactaccaa taccattagc ccacatagac ccaaactggc ctaatcgatc tataaagcat
     6601 atatagtata tactattagg gtctcgctta cgcgaatttc tatttacaag ctccgaaatc
     6661 gaaactgcaa cacaagatag ggaaagaact ggaaatatat actcaacact tgtgtatatc
     6721 ataaatcaca taatccacac aaagcctatg ataattatat ttatatcggt ataatcgtat
     6781 atacatatat agccaaagat tgttgttgaa ctgcgaactg caagttttat taccgttgat
     6841 gatattatct tctgtgtaaa gagaacattt taatcaaacc caagcaattg ttgatgttcg
     6901 gcacgaatga taaccataaa aagaactagc taatcaaaag tcatatgaat actctgttaa
     6961 ctaattactg tttatgtttg ttgatattat ttaattcatt gatattaatg caccgtatgc
     7021 ttttgtgttt aactaacatg agcaaggcat gatgtaggta ctaaaaataa tacttaagta
     7081 gatagccgta gaagcttggc tcgatcgagc atggtcatag ctgtttcctg tgtgaaattg
     7141 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7201 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7261 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7321 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7381 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7441 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7501 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7561 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7621 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7681 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7741 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7801 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7861 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7921 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7981 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8041 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8101 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8161 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8221 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa
     8281 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     8341 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     8401 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     8461 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     8521 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     8581 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     8641 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     8701 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     8761 taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg catcatcagg
     8821 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     8881 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     8941 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     9001 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     9061 gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat
     9121 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9181 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9241 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
