LOCUS       sequence-467246-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 plasmid for MoClo, encodes the GPD1 promoter for 
            K.marxianus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Thornbury M, Knoops A, Summerby-Murray I, Dhaliwal J, Johnson S, 
            Utomo JC, Joshi J, Narcross L, Remondetto G, Pouliot M, Whiteway M, 
            Martin VJJ
  TITLE     Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a 
            Host for Lactose Valorization.
  JOURNAL   ACS Synth Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096.
  PUBMED    40629255
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jul 8. doi: 10.1021/acssynbio.5c00096."
COMMENT     Sequence Label: pMTKM15
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1037..1071)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1142..1801)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1716..1735
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1983..2366,1..205)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
       61 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      301 ccgtagtcgg tctcaaacga aagggacaat cggagaatag aaatcggcat gcccactaag
      361 cacgcaacgt cctcaggttg tccctcaaaa ccgaaaggcc aaacagccca aaataaggcc
      421 aaaaaaggaa tgaattaaaa gaaaagagga caaaattccc gtttccttgg gtgtagcacc
      481 gcatgacggt gaacctaccc ccaaccaaaa cgaaagaagt aacagaaatt gatggtacaa
      541 caaggctgat attgaccaaa tttcccagaa acacccgtac cagatgttcc gctcggttct
      601 gggaaaaata ctagtccctc acccagtccc tcccctagtc cgattctctt ctccttacaa
      661 tttgcctcct gccctttgcc cctttgtata tacagaaaga aaaaaaataa taataatgag
      721 gtattactat gctaaaactg aattgtaatt aactatgtgc agtggccctt tttacgttat
      781 gtatcatcat cgtcagcatc atcattcgcg cgttacactg tttgttggtg ctgtttcagt
      841 tggctctctt ggtttgtcta tctgtttctt ctttttcttg tctatttctt tttctccgaa
      901 acgtagtata tgtacgtatt tggtgttgta ttacatagtt gagccttagc cagtaagagt
      961 ttcggaaggg tacggaaacc aaaagtatat aaagagagtg gaggaatcag ttttcagatt
     1021 atgtgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1081 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1141 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1201 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1261 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1321 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
     1381 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1441 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1501 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1561 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1621 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1681 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1741 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1801 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1861 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
     1921 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1981 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2041 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2101 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2161 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2221 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2281 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2341 acctacaccg aactgagata cctaca
//
